Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.

Detalhes bibliográficos
Autor(a) principal: Silva, Tânia Regina Marques da
Data de Publicação: 2002
Outros Autores: Freitas, Juliana Ribeiro de, Silva, Queilan Chagas, Figueira, Cláudio Pereira, Roxo, Eliana, Leão, Sylvia Cardoso, Freitas, Luiz Antonio Rodrigues de, Veras, Patrícia Sampaio Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7752
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil
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spelling Silva, Tânia Regina Marques daFreitas, Juliana Ribeiro deSilva, Queilan ChagasFigueira, Cláudio PereiraRoxo, ElianaLeão, Sylvia CardosoFreitas, Luiz Antonio Rodrigues deVeras, Patrícia Sampaio Tavares2014-06-02T18:39:25Z2014-06-02T18:39:25Z2002SILVA, T. et al. Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion. Infection and Immunity, v. 70, n. 10, p. 5628-5634, 2002.0019-9567https://www.arca.fiocruz.br/handle/icict/775210.1128/IAI.70.10.5628–5634.2002engAmerican Society for MicrobiologyVirulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahiao. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, BrasilInstituto Biológico. São Paulo, SP, BrasilUniversidade Federal de São Paulo. UNIFESP/EPM. Escola Paulista de Medicina. Departamento de Microbiologia, Imunologia e Parasitologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisa Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil / Faculdade de Medicina. Universidade Federal da Bahia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil /Escola Bahiana de Medicina e Saúde Pública. Salvador, BA, BrasilThe virulence of different isolates of Mycobacterium has been associated with two morphologically distinguishable colonial variants: opaque (SmOp) and transparent (SmTr). In this report we used an in vitro assay to compare macrophage (M ) responses to SmOp and SmTr Mycobacterium fortuitum variants, taking advantage of the fact that these variants were derived from the same isolate. Cells preactivated or not with gamma interferon (IFN- ) were infected with SmOp or SmTr M. fortuitum. We showed that SmOp and SmTr induced different levels of nitric oxide (NO) production by IFN- -stimulatedM . Indeed, the amount of IFN- -induced NO production by J774 cells was 4.8 to 9.0 times higher by SmOp (23.1 to 37.7 M) compared to SmTr infection (3.9 to 4.8 M) (P 0.0332), indicating that virulent SmTr bacilli restricted NO production. In addition, IFN- -induced NO production by M was higher when correlated with reduction of only avirulent SmOp bacillus viability. SNAP (S-nitroso-N-acetyl-DL-penicillamine)-induced NO production did not modify SmTr viability, indicating its resistance to nitrogen radicals. Electron microscopy studies were performed to evaluate the capacity of phagosomes to fuse with lysosomes labeled with bovine serum albumin-colloidal gold particles. By 24 h postinfection, 69% more phagosome-containing SmOp variant had fused with lysosomes compared to the SmTr-induced phagosomes. In conclusion, these data indicate that virulent SmTr bacilli may escape host defense by restricting IFN- -induced NO production, resisting nitrogen toxic radicals, and limiting phagosome fusion with lysosomes.Interferon gama/farmacologiaLisossomos/fisiologiaMacrófagos/microbiologiaMacrófagos/fisiologiaMycobacterium fortuitum/patogenicidadeÓxido Nítrico/biossínteseFagossomos/fisiologiaAnimaisLinhagem CelularVariação GenéticaLisossomos/microbiologiaAtivação de Macrófagos/efeitos de drogasMacrófagos/ultraestruturaFusão de MembranaCamundongosMicroscopia EletrônicaMycobacterium fortuitum/isolamento & purificaçãoFagossomos/microbiologiaProteínas RecombinantesVirulência/genéticainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALSilva TR Virulent mycobacterium....pdfSilva TR Virulent mycobacterium....pdfapplication/pdf1089422https://www.arca.fiocruz.br/bitstream/icict/7752/1/Silva%20TR%20Virulent%20mycobacterium....pdf245e335fcfd9920cd886946e407f5736MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7752/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTSilva TR Virulent mycobacterium....pdf.txtSilva TR Virulent mycobacterium....pdf.txtExtracted texttext/plain42150https://www.arca.fiocruz.br/bitstream/icict/7752/5/Silva%20TR%20Virulent%20mycobacterium....pdf.txt5943b381b73eb1ba3050a4615c554d1fMD55THUMBNAILSilva TR Virulent mycobacterium....pdf.jpgSilva TR Virulent mycobacterium....pdf.jpgGenerated Thumbnailimage/jpeg2355https://www.arca.fiocruz.br/bitstream/icict/7752/4/Silva%20TR%20Virulent%20mycobacterium....pdf.jpg72ba8d2106074f4b790e8d3b4adc9dd2MD54icict/77522023-03-15 14:33:17.309oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:17Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
title Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
spellingShingle Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
Silva, Tânia Regina Marques da
Interferon gama/farmacologia
Lisossomos/fisiologia
Macrófagos/microbiologia
Macrófagos/fisiologia
Mycobacterium fortuitum/patogenicidade
Óxido Nítrico/biossíntese
Fagossomos/fisiologia
Animais
Linhagem Celular
Variação Genética
Lisossomos/microbiologia
Ativação de Macrófagos/efeitos de drogas
Macrófagos/ultraestrutura
Fusão de Membrana
Camundongos
Microscopia Eletrônica
Mycobacterium fortuitum/isolamento & purificação
Fagossomos/microbiologia
Proteínas Recombinantes
Virulência/genética
title_short Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
title_full Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
title_fullStr Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
title_full_unstemmed Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
title_sort Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion.
author Silva, Tânia Regina Marques da
author_facet Silva, Tânia Regina Marques da
Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
author_role author
author2 Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Tânia Regina Marques da
Freitas, Juliana Ribeiro de
Silva, Queilan Chagas
Figueira, Cláudio Pereira
Roxo, Eliana
Leão, Sylvia Cardoso
Freitas, Luiz Antonio Rodrigues de
Veras, Patrícia Sampaio Tavares
dc.subject.decs.pt_BR.fl_str_mv Interferon gama/farmacologia
Lisossomos/fisiologia
Macrófagos/microbiologia
Macrófagos/fisiologia
Mycobacterium fortuitum/patogenicidade
Óxido Nítrico/biossíntese
Fagossomos/fisiologia
Animais
Linhagem Celular
Variação Genética
Lisossomos/microbiologia
Ativação de Macrófagos/efeitos de drogas
Macrófagos/ultraestrutura
Fusão de Membrana
Camundongos
Microscopia Eletrônica
Mycobacterium fortuitum/isolamento & purificação
Fagossomos/microbiologia
Proteínas Recombinantes
Virulência/genética
topic Interferon gama/farmacologia
Lisossomos/fisiologia
Macrófagos/microbiologia
Macrófagos/fisiologia
Mycobacterium fortuitum/patogenicidade
Óxido Nítrico/biossíntese
Fagossomos/fisiologia
Animais
Linhagem Celular
Variação Genética
Lisossomos/microbiologia
Ativação de Macrófagos/efeitos de drogas
Macrófagos/ultraestrutura
Fusão de Membrana
Camundongos
Microscopia Eletrônica
Mycobacterium fortuitum/isolamento & purificação
Fagossomos/microbiologia
Proteínas Recombinantes
Virulência/genética
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Patologia e Biologia Celular. Salvador, BA, Brasil
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2014-06-02T18:39:25Z
dc.date.available.fl_str_mv 2014-06-02T18:39:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILVA, T. et al. Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion. Infection and Immunity, v. 70, n. 10, p. 5628-5634, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7752
dc.identifier.issn.none.fl_str_mv 0019-9567
dc.identifier.doi.none.fl_str_mv 10.1128/IAI.70.10.5628–5634.2002
identifier_str_mv SILVA, T. et al. Virulent Mycobacterium fortuitum restricts NO production by a gamma interferon-activated J774 cell line and phagosome-lysosome fusion. Infection and Immunity, v. 70, n. 10, p. 5628-5634, 2002.
0019-9567
10.1128/IAI.70.10.5628–5634.2002
url https://www.arca.fiocruz.br/handle/icict/7752
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
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