First detection of dengue virus in the saliva of immunocompetent murine model
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26225 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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Rasinhas, Arthur da CostaSilva, Marcos Alexandre Nunes daCaldas, Gabriela CardosoJácome, Fernanda CunhaLeonardo, RaphaelSantos, Flávia Barreto dosNunes, Priscila Conrado GuerraBarth, Ortrud MonikaVieira, Debora Ferreira Barreto2018-05-03T12:03:04Z2018-05-03T12:03:04Z2018RASINHAS, Arthur da Costa; et al. First detection of dengue virus in the saliva of immunocompetent murine model. Mem Inst Oswaldo Cruz, v.113, n.4,: e170208, 2018.0074-0276https://www.arca.fiocruz.br/handle/icict/2622510.1590/0074-027601702081678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzVírus da Dengue tipo 4modelo murino imunocompetentesalivadengue 4salivaBALB/c miceimmunocompetent murine modelFirst detection of dengue virus in the saliva of immunocompetent murine modelinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil.The lack of an experimental animal model for the study of dengue pathogenesis is a limiting factor for the development of vaccines and drugs. In previous studies, our group demonstrated the susceptibility of BALB/c mice to infection by dengue virus (DENV) 1 and 2, and the virus was successfully isolated in several organs. In this study, BALB/c mice were experimentally infected intravenously with DENV-4, and samples of their saliva were collected. Viral RNA extracted from the saliva samples was subjected to qRT-PCR, with a detection limit of 0.002 PFU/mL. The presence of DENV-4 viral RNA was detected in the saliva of two mice, presenting viral titers of 109 RNA/mL. The detection of DENV RNA via saliva sampling is not a common practice in dengue diagnosis, due to the lower detection rates in human patients. However, the results observed in this study seem to indicate that, as in humans, detection rates of DENV RNA in mouse saliva are also low, correlating the infection in both cases. This study reports the first DENV detection in the saliva of BALB/c immunocompetent mice experimentally infected with non-neuroadapted DENV-4.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26225/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALarthur_rasinhas_etal_IOC_2018.pdfarthur_rasinhas_etal_IOC_2018.pdfapplication/pdf485256https://www.arca.fiocruz.br/bitstream/icict/26225/2/arthur_rasinhas_etal_IOC_2018.pdf838d46f9bd7e13f6810194a96cd4719aMD52TEXTarthur_rasinhas_etal_IOC_2018.pdf.txtarthur_rasinhas_etal_IOC_2018.pdf.txtExtracted texttext/plain18028https://www.arca.fiocruz.br/bitstream/icict/26225/3/arthur_rasinhas_etal_IOC_2018.pdf.txt2a07aeb4c82e47e5bb6182be75533741MD53icict/262252023-09-05 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| dc.title.pt_BR.fl_str_mv |
First detection of dengue virus in the saliva of immunocompetent murine model |
| title |
First detection of dengue virus in the saliva of immunocompetent murine model |
| spellingShingle |
First detection of dengue virus in the saliva of immunocompetent murine model Rasinhas, Arthur da Costa Vírus da Dengue tipo 4 modelo murino imunocompetente saliva dengue 4 saliva BALB/c mice immunocompetent murine model |
| title_short |
First detection of dengue virus in the saliva of immunocompetent murine model |
| title_full |
First detection of dengue virus in the saliva of immunocompetent murine model |
| title_fullStr |
First detection of dengue virus in the saliva of immunocompetent murine model |
| title_full_unstemmed |
First detection of dengue virus in the saliva of immunocompetent murine model |
| title_sort |
First detection of dengue virus in the saliva of immunocompetent murine model |
| author |
Rasinhas, Arthur da Costa |
| author_facet |
Rasinhas, Arthur da Costa Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Leonardo, Raphael Santos, Flávia Barreto dos Nunes, Priscila Conrado Guerra Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| author_role |
author |
| author2 |
Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Leonardo, Raphael Santos, Flávia Barreto dos Nunes, Priscila Conrado Guerra Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rasinhas, Arthur da Costa Silva, Marcos Alexandre Nunes da Caldas, Gabriela Cardoso Jácome, Fernanda Cunha Leonardo, Raphael Santos, Flávia Barreto dos Nunes, Priscila Conrado Guerra Barth, Ortrud Monika Vieira, Debora Ferreira Barreto |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus da Dengue tipo 4 modelo murino imunocompetente saliva |
| topic |
Vírus da Dengue tipo 4 modelo murino imunocompetente saliva dengue 4 saliva BALB/c mice immunocompetent murine model |
| dc.subject.en.pt_BR.fl_str_mv |
dengue 4 saliva BALB/c mice immunocompetent murine model |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Morfologia e Morfogênese Viral. Rio de Janeiro, RJ, Brasil. |
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2018 |
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2018-05-03T12:03:04Z |
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2018-05-03T12:03:04Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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RASINHAS, Arthur da Costa; et al. First detection of dengue virus in the saliva of immunocompetent murine model. Mem Inst Oswaldo Cruz, v.113, n.4,: e170208, 2018. |
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https://www.arca.fiocruz.br/handle/icict/26225 |
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0074-0276 |
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10.1590/0074-02760170208 |
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1678-8060 |
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RASINHAS, Arthur da Costa; et al. First detection of dengue virus in the saliva of immunocompetent murine model. Mem Inst Oswaldo Cruz, v.113, n.4,: e170208, 2018. 0074-0276 10.1590/0074-02760170208 1678-8060 |
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