r-Sm14 - pRSETA Efficacy in Experimental Animals

Detalhes bibliográficos
Autor(a) principal: Ramos, Celso Raul Romero
Data de Publicação: 2001
Outros Autores: Vilar, Mônica Magno, Nascimento, Ana Lúcia Tabet Oller, Ho, Paulo Lee, Thaumaturgo, Nilton, Edelenyi, Ricardo, Almeida, Marília, Dias, Waldely de Oliveira, Diogo, Catia Maria, Tendler, Miriam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44170
Resumo: Instituto Butantan, Centro de Biotecnologia. São Paulo, SP, Brasil.
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spelling Ramos, Celso Raul RomeroVilar, Mônica MagnoNascimento, Ana Lúcia Tabet OllerHo, Paulo LeeThaumaturgo, NiltonEdelenyi, RicardoAlmeida, MaríliaDias, Waldely de OliveiraDiogo, Catia MariaTendler, Miriam2020-10-24T18:11:23Z2020-10-24T18:11:23Z2001RAMOS, Celso Raul Romero et al. r-Sm14 - pRSETA Efficacy in Experimental Animals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, Suppl., p. 131-135, 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/4417010.1590/S0074-027620010009000191678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Schistosoma mansoniProteína de ligação de ácido graxoVacina anti-helmintoSchistosoma mansoniFatty acid binding proteinAnti-helminth vacciner-Sm14 - pRSETA Efficacy in Experimental Animalsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleInstituto Butantan, Centro de Biotecnologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil.Instituto Butantan, Centro de Biotecnologia. São Paulo, SP, Brasil.Instituto Butantan, Centro de Biotecnologia. São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil.Universidade Federal de Santa Catarina. MIP-CCB. Florianópolis, SC, Brasil.Universidade de São Paulo., Instituto de Química, São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Helmintologia. Rio de Janeiro, RJ, Brasil.Fasciola hepatica infections were performed with recombinant Sm14 (rSm14) produced in Escherichia coli by the pGEMEX system (Promega). The rSm14 was expressed as a 40 kDa fusion protein with the major bacteriophage T7 capsid protein. Vaccination experiments with this rSm14 in animal models resulted in consistent high protective activity against S. mansoni cercariae challenge and enabled rSm14 to be included among the vaccine antigens endorsed by the World Health Organization for phase I/II clinical trials. Since the preparation of pGEMEX based rSm14 is time consuming and results in low yield for large scale production, we have tested other E. coli expression systems which would be more suitable for scale up and downstream processing. We expressed two different 6XHis-tagged Sm14 fusion proteins in a T7 promoter based plasmids. The 6XHis-tag fusions allowed rapid purification of the recombinant proteins through a Ni+2-charged resin. The resulted recombinant 18 and 16 kDa proteins were recognized by anti-Sm14 antibodies and also by antiserum against adult S. mansoni soluble secreted/excreted proteins in Western-Blot. Both proteins were also protective against S. mansoni cercariae infection to the same extent as the rSm14 expressed by the pGEMEX system.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44170/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdfMonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdfapplication/pdf252604https://www.arca.fiocruz.br/bitstream/icict/44170/2/MonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdfe84e9d21595e32b75069e2ee4c59a216MD52TEXTMonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdf.txtMonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdf.txtExtracted texttext/plain15764https://www.arca.fiocruz.br/bitstream/icict/44170/3/MonicaVilar_RicardoEdelenyi_etal_IOC_2001.pdf.txt42f76daf55243177af228a0f839e8650MD53icict/441702020-10-25 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dc.title.pt_BR.fl_str_mv r-Sm14 - pRSETA Efficacy in Experimental Animals
title r-Sm14 - pRSETA Efficacy in Experimental Animals
spellingShingle r-Sm14 - pRSETA Efficacy in Experimental Animals
Ramos, Celso Raul Romero
Schistosoma mansoni
Proteína de ligação de ácido graxo
Vacina anti-helminto
Schistosoma mansoni
Fatty acid binding protein
Anti-helminth vaccine
title_short r-Sm14 - pRSETA Efficacy in Experimental Animals
title_full r-Sm14 - pRSETA Efficacy in Experimental Animals
title_fullStr r-Sm14 - pRSETA Efficacy in Experimental Animals
title_full_unstemmed r-Sm14 - pRSETA Efficacy in Experimental Animals
title_sort r-Sm14 - pRSETA Efficacy in Experimental Animals
author Ramos, Celso Raul Romero
author_facet Ramos, Celso Raul Romero
Vilar, Mônica Magno
Nascimento, Ana Lúcia Tabet Oller
Ho, Paulo Lee
Thaumaturgo, Nilton
Edelenyi, Ricardo
Almeida, Marília
Dias, Waldely de Oliveira
Diogo, Catia Maria
Tendler, Miriam
author_role author
author2 Vilar, Mônica Magno
Nascimento, Ana Lúcia Tabet Oller
Ho, Paulo Lee
Thaumaturgo, Nilton
Edelenyi, Ricardo
Almeida, Marília
Dias, Waldely de Oliveira
Diogo, Catia Maria
Tendler, Miriam
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Ramos, Celso Raul Romero
Vilar, Mônica Magno
Nascimento, Ana Lúcia Tabet Oller
Ho, Paulo Lee
Thaumaturgo, Nilton
Edelenyi, Ricardo
Almeida, Marília
Dias, Waldely de Oliveira
Diogo, Catia Maria
Tendler, Miriam
dc.subject.other.pt_BR.fl_str_mv Schistosoma mansoni
Proteína de ligação de ácido graxo
Vacina anti-helminto
topic Schistosoma mansoni
Proteína de ligação de ácido graxo
Vacina anti-helminto
Schistosoma mansoni
Fatty acid binding protein
Anti-helminth vaccine
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Fatty acid binding protein
Anti-helminth vaccine
description Instituto Butantan, Centro de Biotecnologia. São Paulo, SP, Brasil.
publishDate 2001
dc.date.issued.fl_str_mv 2001
dc.date.accessioned.fl_str_mv 2020-10-24T18:11:23Z
dc.date.available.fl_str_mv 2020-10-24T18:11:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RAMOS, Celso Raul Romero et al. r-Sm14 - pRSETA Efficacy in Experimental Animals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, Suppl., p. 131-135, 2001.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44170
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762001000900019
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv RAMOS, Celso Raul Romero et al. r-Sm14 - pRSETA Efficacy in Experimental Animals. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 86, Suppl., p. 131-135, 2001.
0074-0276
10.1590/S0074-02762001000900019
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44170
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/44170/1/license.txt
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