Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/53045 |
Resumo: | The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Dept of Physiology, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto.Toronto, ON, Canada. |
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Santos, Claudia C. dosAmatullah, HajeraVaswani, Chirag M.Maron-Gutierrez, TatianaKIm, MichaelMei, Shirley H. J.Szaszi, KatalinMonteiro, Ana Paula T.Varkouhi, Amir KHerreroz, RaquelLorente, Jose AngelTsoporis, James N.Gupta, SahilEktesabi, AminKavantzas, NikolaosSalpeas, VasileiosMarshall, John C.Rocco, Patricia R.M.Marsden, Philip A.Weiss, Daniel J.Stewart, Ducan J.Hu, PingzhaoLiles, W. Conrad2022-06-01T20:36:27Z2022-06-01T20:36:27Z2022SANTOS, Claudia C. dos et al. Mesenchymal stromal (stem) cell therapy modulates miR-193b- 5p expression to attenuate sepsis-induced acute lung injury. European Respiratory Journal, v. 59, 2004216, p. 1 - 15, 2022.0903-1936https://www.arca.fiocruz.br/handle/icict/5304510.1183/13993003.04216-2020engERS PublicationsTerapiaCélulas mesenquimais estromaisModula expressão de miR-193b-5pAtenuar a lesão pulmonar agudaInduzida por sepseMesenchymal stromal (stem)Cell therapyModulates miR-193b- 5p expressionAttenuateAcute lung injurySepsis-inducedMesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injuryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleThe Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Dept of Physiology, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto.Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Dept of Physiology, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.Regenerative Medicine Program, Ottawa Hospital Research Institute. Ottawa, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Dept of Surgery. University of Toronto, Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.University Hospital of Getafe, Critical Care Dept. Madrid, Spain.University Hospital of Getafe, Critical Care Dept. Madrid, Spain / Centros de Investigación Biomédica en Red (CIBER) de Enfermedades Respiratorias. Madrid, Spain / Universidad Europea de Madrid. Madrid, Spain..The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada.1st Dept of Pathology, School of Medicine, National and Kapodistrian, University of Athens. Athens, Greece.1st Dept of Pathology, School of Medicine, National and Kapodistrian, University of Athens. Athens, Greece.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada / Dept of Surgery, University of Toronto. Toronto, ON, Canada.Universidade Federal do Rio de Janeiro. Instituto de Biofisica Carlos Chagas Filho. Laboratório de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada.Dept of Medicine, University of Vermont, Burlington, VT, USA.Regenerative Medicine Program, Ottawa Hospital Research Institute. Ottawa, ON, Canada.Dept of Biochemistry and Medical Genetics, University of Manitoba. Winnipeg, MB, Canada.Dept of Medicine, University of Washington, Seattle, WA, USA.Although mesenchymal stromal (stem) cell (MSC) administration attenuates sepsis-induced lung injury in pre-clinical models, the mechanism(s) of action and host immune system contributions to its therapeutic effects remain elusive. We show that treatment with MSCs decreased expression of host-derived microRNA (miR)-193b-5p and increased expression of its target gene, the tight junctional protein occludin (Ocln), in lungs from septic mice. Mutating the Ocln 3′ untranslated region miR-193b-5p binding sequence impaired binding to Ocln mRNA. Inhibition of miR-193b-5p in human primary pulmonary microvascular endothelial cells prevents tumour necrosis factor (TNF)-induced decrease in Ocln gene and protein expression and loss of barrier function. MSC-conditioned media mitigated TNF-induced miR- 193b-5p upregulation and Ocln downregulation in vitro. When administered in vivo, MSC-conditioned media recapitulated the effects of MSC administration on pulmonary miR-193b-5p and Ocln expression. MiR-193b-deficient mice were resistant to pulmonary inflammation and injury induced by lipopolysaccharide (LPS) instillation. Silencing of Ocln in miR-193b-deficient mice partially recovered the susceptibility to LPS-induced lung injury. In vivo inhibition of miR-193b-5p protected mice from endotoxin-induced lung injury. Finally, the clinical significance of these results was supported by the finding of increased miR-193b-5p expression levels in lung autopsy samples from acute respiratory distress syndrome patients who died with diffuse alveolar damage.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/53045/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTatianaMaron_Gutierrez_etal_IOC_2022.pdfTatianaMaron_Gutierrez_etal_IOC_2022.pdfapplication/pdf1468544https://www.arca.fiocruz.br/bitstream/icict/53045/2/TatianaMaron_Gutierrez_etal_IOC_2022.pdf0f65480409c2260bd9c67c617c679db7MD52icict/530452023-09-14 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| dc.title.pt_BR.fl_str_mv |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| title |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| spellingShingle |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury Santos, Claudia C. dos Terapia Células mesenquimais estromais Modula expressão de miR-193b-5p Atenuar a lesão pulmonar aguda Induzida por sepse Mesenchymal stromal (stem) Cell therapy Modulates miR-193b- 5p expression Attenuate Acute lung injury Sepsis-induced |
| title_short |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| title_full |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| title_fullStr |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| title_full_unstemmed |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| title_sort |
Mesenchymal stromal (stem) cell therapy modulates miR-193b5p expression to attenuate sepsis-induced acute lung injury |
| author |
Santos, Claudia C. dos |
| author_facet |
Santos, Claudia C. dos Amatullah, Hajera Vaswani, Chirag M. Maron-Gutierrez, Tatiana KIm, Michael Mei, Shirley H. J. Szaszi, Katalin Monteiro, Ana Paula T. Varkouhi, Amir K Herreroz, Raquel Lorente, Jose Angel Tsoporis, James N. Gupta, Sahil Ektesabi, Amin Kavantzas, Nikolaos Salpeas, Vasileios Marshall, John C. Rocco, Patricia R.M. Marsden, Philip A. Weiss, Daniel J. Stewart, Ducan J. Hu, Pingzhao Liles, W. Conrad |
| author_role |
author |
| author2 |
Amatullah, Hajera Vaswani, Chirag M. Maron-Gutierrez, Tatiana KIm, Michael Mei, Shirley H. J. Szaszi, Katalin Monteiro, Ana Paula T. Varkouhi, Amir K Herreroz, Raquel Lorente, Jose Angel Tsoporis, James N. Gupta, Sahil Ektesabi, Amin Kavantzas, Nikolaos Salpeas, Vasileios Marshall, John C. Rocco, Patricia R.M. Marsden, Philip A. Weiss, Daniel J. Stewart, Ducan J. Hu, Pingzhao Liles, W. Conrad |
| author2_role |
author author author author author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Santos, Claudia C. dos Amatullah, Hajera Vaswani, Chirag M. Maron-Gutierrez, Tatiana KIm, Michael Mei, Shirley H. J. Szaszi, Katalin Monteiro, Ana Paula T. Varkouhi, Amir K Herreroz, Raquel Lorente, Jose Angel Tsoporis, James N. Gupta, Sahil Ektesabi, Amin Kavantzas, Nikolaos Salpeas, Vasileios Marshall, John C. Rocco, Patricia R.M. Marsden, Philip A. Weiss, Daniel J. Stewart, Ducan J. Hu, Pingzhao Liles, W. Conrad |
| dc.subject.other.pt_BR.fl_str_mv |
Terapia Células mesenquimais estromais Modula expressão de miR-193b-5p Atenuar a lesão pulmonar aguda Induzida por sepse |
| topic |
Terapia Células mesenquimais estromais Modula expressão de miR-193b-5p Atenuar a lesão pulmonar aguda Induzida por sepse Mesenchymal stromal (stem) Cell therapy Modulates miR-193b- 5p expression Attenuate Acute lung injury Sepsis-induced |
| dc.subject.en.pt_BR.fl_str_mv |
Mesenchymal stromal (stem) Cell therapy Modulates miR-193b- 5p expression Attenuate Acute lung injury Sepsis-induced |
| description |
The Keenan Research Centre for Biomedical Science of St Michael’s Hospital. Toronto, ON, Canada / Dept of Physiology, Temerty Faculty of Medicine, University of Toronto. Toronto, ON, Canada / Institute of Medical Sciences and Interdepartmental Division of Critical Care, Temerty Faculty of Medicine, University of Toronto.Toronto, ON, Canada. |
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2022 |
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2022-06-01T20:36:27Z |
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2022-06-01T20:36:27Z |
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2022 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SANTOS, Claudia C. dos et al. Mesenchymal stromal (stem) cell therapy modulates miR-193b- 5p expression to attenuate sepsis-induced acute lung injury. European Respiratory Journal, v. 59, 2004216, p. 1 - 15, 2022. |
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https://www.arca.fiocruz.br/handle/icict/53045 |
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0903-1936 |
| dc.identifier.doi.none.fl_str_mv |
10.1183/13993003.04216-2020 |
| identifier_str_mv |
SANTOS, Claudia C. dos et al. Mesenchymal stromal (stem) cell therapy modulates miR-193b- 5p expression to attenuate sepsis-induced acute lung injury. European Respiratory Journal, v. 59, 2004216, p. 1 - 15, 2022. 0903-1936 10.1183/13993003.04216-2020 |
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ERS Publications |
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ERS Publications |
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