Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice

Detalhes bibliográficos
Autor(a) principal: Roffê, Ester
Data de Publicação: 2007
Outros Autores: Souza, Adriano Luís Soares de, Machado, P. P., Barcelos, L. S., Romanha, Álvaro José, Mariano, F.S., Silva, J. S., Machado, C. R., Tanowitz, H. B., Teixeira, Mauro Martins
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/6635
Resumo: CAPES, FAPEMIG, and NIH/FIRCA (1 R03-TW006857-01A1, USA).
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spelling Roffê, EsterSouza, Adriano Luís Soares deMachado, P. P.Barcelos, L. S.Romanha, Álvaro JoséMariano, F.S.Silva, J. S.Machado, C. R.Tanowitz, H. B.Teixeira, Mauro Martins2013-07-02T12:53:40Z2013-07-02T12:53:40Z2007Roffê, E et al. Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice. Braz J Med Biol Res. 2007 Mar;40(3):391-9.0100-879Xhttps://www.arca.fiocruz.br/handle/icict/6635CAPES, FAPEMIG, and NIH/FIRCA (1 R03-TW006857-01A1, USA).Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, BrasilUniversidade de São Paulo. Faculdade de Medicina de Ribeirão Preto. Departamento de Bioquímica e Imunologia. Ribeirão Preto, SP, BrasilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Morfologia. Belo Horizonte, MG, BrasilAlbert Einstein College of Medicine. Bronx, NY, USA/Montefiore Medical Center. Bronx, NY, USAUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrasilChagas’ disease, caused by the protozoan Trypanosoma cruzi, is a major cause of cardiovascular disability in countries where it is endemic. Damage to the heart microvasculature has been proposed to be an important factor in the pathogenesis of heart dysfunction. Endothelin-1 (ET-1) is a potent vasoconstrictor and exerts its effects via specific ETAand ETBreceptors. A few studies have suggested a role for ET-1 and its receptors in the pathogenesis of Chagas’ disease. We investigated the effects of treatment with bosentan, an ETA/ETB receptor antagonist, on the course of T. cruzi infection (Y strain) in C57Bl/6 mice. Treatment with bosentan (100 mg kg-1 day -1 ) was given per osstarting day 0 after infection until sacrifice. Bosentan signifi-cantly increased myocardial inflammation, with no effects on parasi-temia. Although the total number of nests was similar, a lower number of intact amastigote nests was found in the heart of bosentan-treated animals. Bosentan failed to affect the infection-associated increase in the cardiac levels of the cytokines IFN-γand TNF-αand the chemo-kines CCL2/MCP-1, CCL3/MIP-1αand CCL5/RANTES. In vitro, pre-incubation with ET-1 (0.1 µM) 4 h before infection enhanced the uptake of the parasites by peritoneal macrophages, and this effect was abrogated when macrophages were pre-treated with bosentan (1 µM) 15 min before incubation with ET-1. However, ET-1 did not alter killing of intracellular parasites after 48 h of in vitroinfection. Our data suggest that bosentan-treated mice have a delay in controlling parasitism which is compensated for exacerbated inflammation. In-fection is eventually controlled in these animals and lethality is unchanged, demonstrating that ET-1 plays a minor role in the protec-tion against acute murine T. cruziinfection.engEndothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrypanosoma cruziEndothelinMyocarditis Chemokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL95 ROFFÊ E.pdf95 ROFFÊ E.pdfapplication/pdf922671https://www.arca.fiocruz.br/bitstream/icict/6635/1/95%20ROFF%c3%8a%20E.pdfb3e076ae46ce1630ee26f48ce4c5a562MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/6635/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXT95 ROFFÊ E.pdf.txt95 ROFFÊ E.pdf.txtExtracted texttext/plain31229https://www.arca.fiocruz.br/bitstream/icict/6635/5/95%20ROFF%c3%8a%20E.pdf.txtb1aaaa4341a01d6da296cb1be102cd2bMD55THUMBNAIL95 ROFFÊ E.pdf.jpg95 ROFFÊ E.pdf.jpgGenerated Thumbnailimage/jpeg1678https://www.arca.fiocruz.br/bitstream/icict/6635/4/95%20ROFF%c3%8a%20E.pdf.jpg66337e0e24a44a7c640374364fdb2e69MD54icict/66352018-04-25 13:15:05.27oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-25T16:15:05Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
title Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
spellingShingle Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
Roffê, Ester
Trypanosoma cruzi
Endothelin
Myocarditis Chemokines
title_short Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
title_full Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
title_fullStr Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
title_full_unstemmed Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
title_sort Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice
author Roffê, Ester
author_facet Roffê, Ester
Souza, Adriano Luís Soares de
Machado, P. P.
Barcelos, L. S.
Romanha, Álvaro José
Mariano, F.S.
Silva, J. S.
Machado, C. R.
Tanowitz, H. B.
Teixeira, Mauro Martins
author_role author
author2 Souza, Adriano Luís Soares de
Machado, P. P.
Barcelos, L. S.
Romanha, Álvaro José
Mariano, F.S.
Silva, J. S.
Machado, C. R.
Tanowitz, H. B.
Teixeira, Mauro Martins
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Roffê, Ester
Souza, Adriano Luís Soares de
Machado, P. P.
Barcelos, L. S.
Romanha, Álvaro José
Mariano, F.S.
Silva, J. S.
Machado, C. R.
Tanowitz, H. B.
Teixeira, Mauro Martins
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
Endothelin
Myocarditis Chemokines
topic Trypanosoma cruzi
Endothelin
Myocarditis Chemokines
description CAPES, FAPEMIG, and NIH/FIRCA (1 R03-TW006857-01A1, USA).
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2013-07-02T12:53:40Z
dc.date.available.fl_str_mv 2013-07-02T12:53:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv Roffê, E et al. Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice. Braz J Med Biol Res. 2007 Mar;40(3):391-9.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/6635
dc.identifier.issn.none.fl_str_mv 0100-879X
identifier_str_mv Roffê, E et al. Endothelin-1 receptors play a minor role in the protection against acute Trypanosoma cruzi infection in mice. Braz J Med Biol Res. 2007 Mar;40(3):391-9.
0100-879X
url https://www.arca.fiocruz.br/handle/icict/6635
dc.language.iso.fl_str_mv eng
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