How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily

Detalhes bibliográficos
Autor(a) principal: Morais, Mariana Abrahão Bueno de
Data de Publicação: 2015
Outros Autores: Giuseppe, Priscila Oliveira de, Souza, Tatiana de Arruda Campos Brasil de, Alegria, Thiago G. P., Oliveira, Marcos A., Soares Netto, Luis Eduardo, Murakami, Mario Tyago
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15508
Resumo: Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grants 2010/51730-0, 2011/10248-4, and 2012/24134-3
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spelling Morais, Mariana Abrahão Bueno deGiuseppe, Priscila Oliveira deSouza, Tatiana de Arruda Campos Brasil deAlegria, Thiago G. P.Oliveira, Marcos A.Soares Netto, Luis EduardoMurakami, Mario Tyago2016-08-29T19:07:50Z2016-08-29T19:07:50Z2015MORAIS, Mariana Abrahão Bueno de et al. How pH modulates the dimer-decamer interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily. Journal of Biological Chemistry. v. 290, n. 13, p. 8582-8590, 27 Mar. 2015.0021-9258https://www.arca.fiocruz.br/handle/icict/1550810.1074/jbc.M114.6192051083-351XFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grants 2010/51730-0, 2011/10248-4, and 2012/24134-3Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Proteômica e Engenharia de Proteínas. Curitiba, PR, Brasil.Universidade de São Paulo. Instituto de Biociências. Departamento de Genética e Biologia Evolutiva. São Paulo, SP, Brasil.Universidade Estadual Paulista Júlio de Mesquita Filho. Departamento de Biologia. São Vicente, SP, Brasil.Universidade de São Paulo. Instituto de Biociências. Departamento de Genética e Biologia Evolutiva. São Paulo, SP, Brasil.Centro Nacional de Pesquisa em Energia e Materiais. Laboratório Nacional de Biociências. Campinas, SP, Brasil.2-Cys peroxiredoxins belonging to the Prx1 subfamily are Cys-based peroxidases that control the intracellular levels of H₂O₂ and seem to assume a chaperone function under oxidative stress conditions. The regulation of their peroxidase activity as well as the observed functional switch from peroxidase to chaperone involves changes in their quaternary structure. Multiple factors can modulate the oligomeric transitions of 2-Cys peroxiredoxins such as redox state, post-translational modifications, and pH. However, the molecular basis for the pH influence on the oligomeric state of these enzymes is still elusive. Herein, we solved the crystal structure of a typical 2-Cys peroxiredoxin from Leishmania in the dimeric (pH 8.5) and decameric (pH 4.4) forms, showing that conformational changes in the catalytic loop are associated with the pH-induced decamerization. Mutagenesis and biophysical studies revealed that a highly conserved histidine (His¹¹³) functions as a pH sensor that, at acidic conditions, becomes protonated and forms an electrostatic pair with Asp⁷⁶ from the catalytic loop, triggering the decamerization. In these 2-Cys peroxiredoxins, decamer formation is important for the catalytic efficiency and has been associated with an enhanced sensitivity to oxidative inactivation by overoxidation of the peroxidatic cysteine. In eukaryotic cells, exposure to high levels of H₂O₂ can trigger intracellular pH variations, suggesting that pH changes might act cooperatively with H₂O₂ and other oligomerization-modulator factors to regulate the structure and function of typical 2-Cys peroxiredoxins in response to oxidative stress.engHow pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamilyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleConformational changeCrystal structureLeishmaniaPeroxiredoxinpH regulationinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15508/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALHow pH Modulates the Dimer-Decamer Interconversion.pdfHow pH Modulates the Dimer-Decamer Interconversion.pdfapplication/pdf2302516https://www.arca.fiocruz.br/bitstream/icict/15508/2/How%20pH%20Modulates%20the%20Dimer-Decamer%20Interconversion.pdfdf9d3fa1849af6068d929ecea2c0b8c4MD52TEXTHow pH Modulates the Dimer-Decamer Interconversion.pdf.txtHow pH Modulates the Dimer-Decamer Interconversion.pdf.txtExtracted texttext/plain48684https://www.arca.fiocruz.br/bitstream/icict/15508/3/How%20pH%20Modulates%20the%20Dimer-Decamer%20Interconversion.pdf.txt86efa40214049a284b5c015a00d7b0e8MD53icict/155082021-10-08 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dc.title.en.fl_str_mv How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
title How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
spellingShingle How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
Morais, Mariana Abrahão Bueno de
Conformational change
Crystal structure
Leishmania
Peroxiredoxin
pH regulation
title_short How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
title_full How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
title_fullStr How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
title_full_unstemmed How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
title_sort How pH modulates the dimer-decamer interconversion of 2-Cys peroxiredoxins from the Prx1 subfamily
author Morais, Mariana Abrahão Bueno de
author_facet Morais, Mariana Abrahão Bueno de
Giuseppe, Priscila Oliveira de
Souza, Tatiana de Arruda Campos Brasil de
Alegria, Thiago G. P.
Oliveira, Marcos A.
Soares Netto, Luis Eduardo
Murakami, Mario Tyago
author_role author
author2 Giuseppe, Priscila Oliveira de
Souza, Tatiana de Arruda Campos Brasil de
Alegria, Thiago G. P.
Oliveira, Marcos A.
Soares Netto, Luis Eduardo
Murakami, Mario Tyago
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Morais, Mariana Abrahão Bueno de
Giuseppe, Priscila Oliveira de
Souza, Tatiana de Arruda Campos Brasil de
Alegria, Thiago G. P.
Oliveira, Marcos A.
Soares Netto, Luis Eduardo
Murakami, Mario Tyago
dc.subject.en.en.fl_str_mv Conformational change
Crystal structure
Leishmania
Peroxiredoxin
pH regulation
topic Conformational change
Crystal structure
Leishmania
Peroxiredoxin
pH regulation
description Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Grants 2010/51730-0, 2011/10248-4, and 2012/24134-3
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-08-29T19:07:50Z
dc.date.available.fl_str_mv 2016-08-29T19:07:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MORAIS, Mariana Abrahão Bueno de et al. How pH modulates the dimer-decamer interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily. Journal of Biological Chemistry. v. 290, n. 13, p. 8582-8590, 27 Mar. 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15508
dc.identifier.issn.pt_BR.fl_str_mv 0021-9258
dc.identifier.doi.pt_BR.fl_str_mv 10.1074/jbc.M114.619205
dc.identifier.eissn.pt_BR.fl_str_mv 1083-351X
identifier_str_mv MORAIS, Mariana Abrahão Bueno de et al. How pH modulates the dimer-decamer interconversion of 2-Cys Peroxiredoxins from the Prx1 Subfamily. Journal of Biological Chemistry. v. 290, n. 13, p. 8582-8590, 27 Mar. 2015.
0021-9258
10.1074/jbc.M114.619205
1083-351X
url https://www.arca.fiocruz.br/handle/icict/15508
dc.language.iso.fl_str_mv eng
language eng
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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