Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus

Detalhes bibliográficos
Autor(a) principal: Evangelista,Cláudia Carolina Silva
Data de Publicação: 2017
Outros Autores: Guidelli,Giovanna Vieira, Borges,Gustavo, Araujo,Thais Fenz, Souza,Tiago Alves Jorge de, Neves,Ubiraci Pereira da Costa, Tunnacliffe,Alan, Pereira,Tiago Campos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790
Resumo: Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.
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spelling Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbusperoxiredoxinkinaseRNAiproteostasisAbstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.Sociedade Brasileira de Genética2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790Genetics and Molecular Biology v.40 n.4 2017reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/1678-4685-gmb-2017-0030info:eu-repo/semantics/openAccessEvangelista,Cláudia Carolina SilvaGuidelli,Giovanna VieiraBorges,GustavoAraujo,Thais FenzSouza,Tiago Alves Jorge deNeves,Ubiraci Pereira da CostaTunnacliffe,AlanPereira,Tiago Camposeng2017-11-21T00:00:00Zoai:scielo:S1415-47572017000500790Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2017-11-21T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
title Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
spellingShingle Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
Evangelista,Cláudia Carolina Silva
peroxiredoxin
kinase
RNAi
proteostasis
title_short Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
title_full Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
title_fullStr Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
title_full_unstemmed Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
title_sort Multiple genes contribute to anhydrobiosis (tolerance to extreme desiccation) in the nematode Panagrolaimus superbus
author Evangelista,Cláudia Carolina Silva
author_facet Evangelista,Cláudia Carolina Silva
Guidelli,Giovanna Vieira
Borges,Gustavo
Araujo,Thais Fenz
Souza,Tiago Alves Jorge de
Neves,Ubiraci Pereira da Costa
Tunnacliffe,Alan
Pereira,Tiago Campos
author_role author
author2 Guidelli,Giovanna Vieira
Borges,Gustavo
Araujo,Thais Fenz
Souza,Tiago Alves Jorge de
Neves,Ubiraci Pereira da Costa
Tunnacliffe,Alan
Pereira,Tiago Campos
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Evangelista,Cláudia Carolina Silva
Guidelli,Giovanna Vieira
Borges,Gustavo
Araujo,Thais Fenz
Souza,Tiago Alves Jorge de
Neves,Ubiraci Pereira da Costa
Tunnacliffe,Alan
Pereira,Tiago Campos
dc.subject.por.fl_str_mv peroxiredoxin
kinase
RNAi
proteostasis
topic peroxiredoxin
kinase
RNAi
proteostasis
description Abstract The molecular basis of anhydrobiosis, the state of suspended animation entered by some species during extreme desiccation, is still poorly understood despite a number of transcriptome and proteome studies. We therefore conducted functional screening by RNA interference (RNAi) for genes involved in anhydrobiosis in the holo-anhydrobiotic nematode Panagrolaimus superbus. A new method of survival analysis, based on staining, and proof-of-principle RNAi experiments confirmed a role for genes involved in oxidative stress tolerance, while a novel medium-scale RNAi workflow identified a further 40 anhydrobiosis-associated genes, including several involved in proteostasis, DNA repair and signal transduction pathways. This suggests that multiple genes contribute to anhydrobiosis in P. superbus.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500790
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1678-4685-gmb-2017-0030
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.40 n.4 2017
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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