Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence

Detalhes bibliográficos
Autor(a) principal: Fischer, Carlo
Data de Publicação: 2020
Outros Autores: Bozza, Fernando, Merino, Xiomara Jeanleny, Pedroso, Celia, de Oliveira Filho, Edmilson F, Moreira-Soto, Andrés, Schwalb, Alvaro, de Lamballerie, Xavier, Martins Netto, Eduardo, Bozza, Patrícia T, Sarno, Manoel, Brites, Carlos, Gotuzzo, Eduardo, Talledo, Michael, Drexler, Jan Felix
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/40395
Resumo: Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany.
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spelling Fischer, CarloBozza, FernandoMerino, Xiomara JeanlenyPedroso, Celiade Oliveira Filho, Edmilson FMoreira-Soto, AndrésSchwalb, Alvarode Lamballerie, XavierMartins Netto, EduardoBozza, Patrícia TSarno, ManoelBrites, CarlosGotuzzo, EduardoTalledo, MichaelDrexler, Jan Felix2020-03-18T20:14:23Z2020-03-18T20:14:23Z2020FISHER, Carlo et al. Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence. mSphere, v. 5, n. 1. p. e00915-19, 2020.2379-5042https://www.arca.fiocruz.br/handle/icict/4039510.1128/mSphere.00915-19engAmerican Society for MicrobiologyRobustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergenceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleCharité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Medicina Intensiva. Rio de Janeiro, RJ, Brasil.Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru./ Departamento de Medicina, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, PeruComplexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal de Bahia, Salvador, Brazil.Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany.Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany.Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru./ Departamento de Medicina, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, PeruUnité des Virus Émergents, Aix-Marseille University, IRD 190, INSERM 1207, IHU Méditerranée Infection, Marseille, France.Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal de Bahia, Salvador, Brazil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal de Bahia, Salvador, Brazil.Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal de Bahia, Salvador, Brazil.Instituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru./ Departamento de Medicina, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, PeruInstituto de Medicina Tropical Alexander von Humboldt, Universidad Peruana Cayetano Heredia, Lima, Peru./ Departamento de Medicina, Facultad de Medicina, Universidad Peruana Cayetano Heredia, Lima, PeruCharité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany./ German Centre for Infection Research./ Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, Sechenov University, Moscow, Russia.Since 2013, the arthropod-borne Chikungunya virus (CHIKV) has cocirculated with the autochthonous Mayaro virus (MAYV) in Latin America. Both belong to the same alphavirus serocomplex, termed the Semliki Forest serocomplex. The extent of antibody cross-reactivity due to the antigenic relatedness of CHIKV and MAYV in commonly used serologic tests remains unclear. By testing 64 CHIKV- and 37 MAYV-specific sera from cohort studies conducted in Peru and Brazil, we demonstrate about 50% false-positive test results using commercially available enzyme-linked immunosorbent assays (ELISAs) based on structural antigens. In contrast, combining ELISAs for CHIKV and MAYV significantly increased positive predictive values (PPV) among all cohorts from 35.3% to 88.2% for IgM and from 61.3% to 96.8% for IgG (P < 0.0001). Testing of longitudinally collected CHIKV-specific patient sera indicated that ELISA specificity is highest for IgM testing at 5 to 9 days post-onset of symptoms (dpo) and for IgG testing at 10 to 14 dpo. IgG cross-reactivity in ELISA was asymmetric, occurring in 57.9% of MAYV-specific sera compared to 29.5% of CHIKV-specific sera. Parallel plaque reduction neutralization testing (PRNT) for CHIKV and MAYV increased the PPV from 80.0% to 100% (P = 0.0053). However, labor-intense procedures and delayed seroconversion limit PRNT for patient diagnostics. In sum, individual testing for CHIKV or MAYV only is prone to misclassifications that dramatically impact patient diagnostics and sero-epidemiologic investigation. Parallel ELISAs for both CHIKV and MAYV provide an easy and efficient solution to differentiate CHIKV from MAYV infections. This approach may provide a template globally for settings in which alphavirus coemergence imposes similar problems.IMPORTANCE Geographically overlapping transmission of Chikungunya virus (CHIKV) and Mayaro virus (MAYV) in Latin America challenges serologic diagnostics and epidemiologic surveillance, as antibodies against the antigenically related viruses can be cross-reactive, potentially causing false-positive test results. We examined whether widely used ELISAs and plaque reduction neutralization testing allow specific antibody detection in the scenario of CHIKV and MAYV coemergence. For this purpose, we used 37 patient-derived MAYV-specific sera from Peru and 64 patient-derived CHIKV-specific sera from Brazil, including longitudinally collected samples. Extensive testing of those samples revealed strong antibody cross-reactivity in ELISAs, particularly for IgM, which is commonly used for patient diagnostics. Cross-neutralization was also observed, albeit at lower frequencies. Parallel testing for both viruses and comparison of ELISA reactivities and neutralizing antibody titers significantly increased diagnostic specificity. Our data provide a convenient and practicable solution to ensure robust differentiation of CHIKV- and MAYV-specific antibodies.BrazilELISAPeruArbovirus diagnosticsCross-reactivityMosquito-borne diseaseOutbreakSerologyinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83099https://www.arca.fiocruz.br/bitstream/icict/40395/1/license.txt586c046dcfeef936e32f0323bb9a47c0MD51ORIGINALRobustness_Fernando_Bozza_etal_INI_2020.pdfRobustness_Fernando_Bozza_etal_INI_2020.pdfapplication/pdf1216116https://www.arca.fiocruz.br/bitstream/icict/40395/2/Robustness_Fernando_Bozza_etal_INI_2020.pdffdd920d4c1e56626febd2fbc50a8904dMD52TEXTRobustness_Fernando_Bozza_etal_INI_2020.pdf.txtRobustness_Fernando_Bozza_etal_INI_2020.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
title Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
spellingShingle Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
Fischer, Carlo
Brazil
ELISA
Peru
Arbovirus diagnostics
Cross-reactivity
Mosquito-borne disease
Outbreak
Serology
title_short Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
title_full Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
title_fullStr Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
title_full_unstemmed Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
title_sort Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence
author Fischer, Carlo
author_facet Fischer, Carlo
Bozza, Fernando
Merino, Xiomara Jeanleny
Pedroso, Celia
de Oliveira Filho, Edmilson F
Moreira-Soto, Andrés
Schwalb, Alvaro
de Lamballerie, Xavier
Martins Netto, Eduardo
Bozza, Patrícia T
Sarno, Manoel
Brites, Carlos
Gotuzzo, Eduardo
Talledo, Michael
Drexler, Jan Felix
author_role author
author2 Bozza, Fernando
Merino, Xiomara Jeanleny
Pedroso, Celia
de Oliveira Filho, Edmilson F
Moreira-Soto, Andrés
Schwalb, Alvaro
de Lamballerie, Xavier
Martins Netto, Eduardo
Bozza, Patrícia T
Sarno, Manoel
Brites, Carlos
Gotuzzo, Eduardo
Talledo, Michael
Drexler, Jan Felix
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fischer, Carlo
Bozza, Fernando
Merino, Xiomara Jeanleny
Pedroso, Celia
de Oliveira Filho, Edmilson F
Moreira-Soto, Andrés
Schwalb, Alvaro
de Lamballerie, Xavier
Martins Netto, Eduardo
Bozza, Patrícia T
Sarno, Manoel
Brites, Carlos
Gotuzzo, Eduardo
Talledo, Michael
Drexler, Jan Felix
dc.subject.en.pt_BR.fl_str_mv Brazil
ELISA
Peru
Arbovirus diagnostics
Cross-reactivity
Mosquito-borne disease
Outbreak
Serology
topic Brazil
ELISA
Peru
Arbovirus diagnostics
Cross-reactivity
Mosquito-borne disease
Outbreak
Serology
description Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humbolt-Universität zu Berlin and Berlin Institute of Health, Institute of Virology, Berlin, Germany.
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-03-18T20:14:23Z
dc.date.available.fl_str_mv 2020-03-18T20:14:23Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FISHER, Carlo et al. Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence. mSphere, v. 5, n. 1. p. e00915-19, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/40395
dc.identifier.issn.pt_BR.fl_str_mv 2379-5042
dc.identifier.doi.none.fl_str_mv 10.1128/mSphere.00915-19
identifier_str_mv FISHER, Carlo et al. Robustness of Serologic Investigations for Chikungunya and Mayaro Viruses following Coemergence. mSphere, v. 5, n. 1. p. e00915-19, 2020.
2379-5042
10.1128/mSphere.00915-19
url https://www.arca.fiocruz.br/handle/icict/40395
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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