Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa

Detalhes bibliográficos
Autor(a) principal: Oliveira, GH [UNESP]
Data de Publicação: 2006
Outros Autores: Berchieri Jr, A [UNESP], Montassier, Helio Jose [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1516-635X2006000100008
http://hdl.handle.net/11449/2927
Resumo: This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
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spelling Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by ElisaChickenELISAenzymeSalmonella spSerologyThis study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.UNESP FCAV Department of Animal PathologyUNESP FCAV Department of Animal PathologyFundação APINCO de Ciência e Tecnologia AvícolasUniversidade Estadual Paulista (Unesp)Oliveira, GH [UNESP]Berchieri Jr, A [UNESP]Montassier, Helio Jose [UNESP]2014-05-20T13:15:55Z2014-05-20T13:15:55Z2006-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article51-54application/pdfhttp://dx.doi.org/10.1590/S1516-635X2006000100008Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.1516-635Xhttp://hdl.handle.net/11449/292710.1590/S1516-635X2006000100008S1516-635X2006000100008S1516-635X2006000100008.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Brasileira de Ciência Avícola0.463info:eu-repo/semantics/openAccess2023-10-14T06:05:00Zoai:repositorio.unesp.br:11449/2927Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-14T06:05Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
spellingShingle Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
Oliveira, GH [UNESP]
Chicken
ELISA
enzyme
Salmonella sp
Serology
title_short Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_full Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_fullStr Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_full_unstemmed Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
title_sort Chicken serologic response to Salmonella enterica serotype Typhimurium assessed by Elisa
author Oliveira, GH [UNESP]
author_facet Oliveira, GH [UNESP]
Berchieri Jr, A [UNESP]
Montassier, Helio Jose [UNESP]
author_role author
author2 Berchieri Jr, A [UNESP]
Montassier, Helio Jose [UNESP]
author2_role author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv Oliveira, GH [UNESP]
Berchieri Jr, A [UNESP]
Montassier, Helio Jose [UNESP]
dc.subject.por.fl_str_mv Chicken
ELISA
enzyme
Salmonella sp
Serology
topic Chicken
ELISA
enzyme
Salmonella sp
Serology
description This study evaluated two enzyme-linked immunosorbent assays (ELISA) in the detection of chicken serologic response against Salmonella enterica sorotype Typhimurium. The assays have used as detecting antigen the soluble bacterial proteins of a non-flagellated strain of Salmonella Typhimurium (AgTM), and antibody conjugated to peroxidase or alkaline phosphatase. According to the results, optimal dilutions of antigen (concentration 5.49 mg/mL) and serum samples in both assays were 1:20,000 and 1:1,000, respectively. In such conditions, the ELISA/AgTM was able to detect serological response to Salmonella Typhimurium. Cross-reactions to Salmonella serotypes Gallinarum and Pullorum were seen, but not with other serotypes such as Enteritidis.
publishDate 2006
dc.date.none.fl_str_mv 2006-03-01
2014-05-20T13:15:55Z
2014-05-20T13:15:55Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1516-635X2006000100008
Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.
1516-635X
http://hdl.handle.net/11449/2927
10.1590/S1516-635X2006000100008
S1516-635X2006000100008
S1516-635X2006000100008.pdf
url http://dx.doi.org/10.1590/S1516-635X2006000100008
http://hdl.handle.net/11449/2927
identifier_str_mv Revista Brasileira de Ciência Avícola. Fundação APINCO de Ciência e Tecnologia Avícolas, v. 8, n. 1, p. 51-54, 2006.
1516-635X
10.1590/S1516-635X2006000100008
S1516-635X2006000100008
S1516-635X2006000100008.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Revista Brasileira de Ciência Avícola
0.463
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 51-54
application/pdf
dc.publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
publisher.none.fl_str_mv Fundação APINCO de Ciência e Tecnologia Avícolas
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1799964555953045504

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