A novel Hepatitis C Virus genotyping method based on liquid microarray

Detalhes bibliográficos
Autor(a) principal: Duarte, Cesar A. B.
Data de Publicação: 2010
Outros Autores: Foti, Leonardo, Nakatani, Sueli M., Riediger, Irina Nastassja, Poersch, Celina O., Pavoni, Daniela Parada, Krieger, Marco Aurélio
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/30031
Resumo: Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
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spelling Duarte, Cesar A. B.Foti, LeonardoNakatani, Sueli M.Riediger, Irina NastassjaPoersch, Celina O.Pavoni, Daniela ParadaKrieger, Marco Aurélio2018-11-14T14:54:49Z2018-11-14T14:54:49Z2010DUARTE, César A. B. et al. A novel Hepatitis C Virus genotyping method based on liquid microarray. PLoS ONE, v. 5, n. 9, p. 1-7, 2010.1932-6203https://www.arca.fiocruz.br/handle/icict/3003110.1371/journal.pone.00128221932-6203porPublic Library of ScienceA novel Hepatitis C Virus genotyping method based on liquid microarrayinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Escola de Medicina. Departamento de Gastroenterologia.São Paulo, SP, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.The strategy used to treat HCV infection depends on the genotype involved. An accurate and reliable genotyping method is therefore of paramount importance. We describe here, for the first time, the use of a liquid microarray for HCV genotyping. This liquid microarray is based on the 5'UTR - the most highly conserved region of HCV - and the variable region NS5B sequence. The simultaneous genotyping of two regions can be used to confirm findings and should detect inter-genotypic recombination. Plasma samples from 78 patients infected with viruses with genotypes and subtypes determined in the Versant™ HCV Genotype Assay LiPA (version I; Siemens Medical Solutions, Diagnostics Division, Fernwald, Germany) were tested with our new liquid microarray method. This method successfully determined the genotypes of 74 of the 78 samples previously genotyped in the Versant™ HCV Genotype Assay LiPA (74/78, 95%). The concordance between the two methods was 100% for genotype determination (74/74). At the subtype level, all 3a and 2b samples gave identical results with both methods (17/17 and 7/7, respectively). Two 2c samples were correctly identified by microarray, but could only be determined to the genotype level with the Versant™ HCV assay. Genotype "1" subtypes (1a and 1b) were correctly identified by the Versant™ HCV assay and the microarray in 68% and 40% of cases, respectively. No genotype discordance was found for any sample. HCV was successfully genotyped with both methods, and this is of prime importance for treatment planning. Liquid microarray assays may therefore be added to the list of methods suitable for HCV genotyping. It provides comparable results and may readily be adapted for the detection of other viruses frequently co-infecting HCV patients. Liquid array technology is thus a reliable and promising platform for HCV genotyping.Hepatitis CMicroarray AnalysisGenotyping TechniquesAnálisis por MicromatricesTécnicas de GenotipajeHepacivirusHepatite CAnálise em MicrossériesTécnicas de Genotipageminfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/30031/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALPlosone.00128.pdfPlosone.00128.pdfapplication/pdf173737https://www.arca.fiocruz.br/bitstream/icict/30031/2/Plosone.00128.pdf193d7421c3a561bad6c443feb5da3e5dMD52TEXTPlosone.00128.pdf.txtPlosone.00128.pdf.txtExtracted texttext/plain37475https://www.arca.fiocruz.br/bitstream/icict/30031/3/Plosone.00128.pdf.txt46b57b6f6a3f97131054c25d62c51823MD53icict/300312019-11-23 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dc.title.pt_BR.fl_str_mv A novel Hepatitis C Virus genotyping method based on liquid microarray
title A novel Hepatitis C Virus genotyping method based on liquid microarray
spellingShingle A novel Hepatitis C Virus genotyping method based on liquid microarray
Duarte, Cesar A. B.
Hepatitis C
Microarray Analysis
Genotyping Techniques
Análisis por Micromatrices
Técnicas de Genotipaje
Hepacivirus
Hepatite C
Análise em Microsséries
Técnicas de Genotipagem
title_short A novel Hepatitis C Virus genotyping method based on liquid microarray
title_full A novel Hepatitis C Virus genotyping method based on liquid microarray
title_fullStr A novel Hepatitis C Virus genotyping method based on liquid microarray
title_full_unstemmed A novel Hepatitis C Virus genotyping method based on liquid microarray
title_sort A novel Hepatitis C Virus genotyping method based on liquid microarray
author Duarte, Cesar A. B.
author_facet Duarte, Cesar A. B.
Foti, Leonardo
Nakatani, Sueli M.
Riediger, Irina Nastassja
Poersch, Celina O.
Pavoni, Daniela Parada
Krieger, Marco Aurélio
author_role author
author2 Foti, Leonardo
Nakatani, Sueli M.
Riediger, Irina Nastassja
Poersch, Celina O.
Pavoni, Daniela Parada
Krieger, Marco Aurélio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Duarte, Cesar A. B.
Foti, Leonardo
Nakatani, Sueli M.
Riediger, Irina Nastassja
Poersch, Celina O.
Pavoni, Daniela Parada
Krieger, Marco Aurélio
dc.subject.en.pt_BR.fl_str_mv Hepatitis C
Microarray Analysis
Genotyping Techniques
topic Hepatitis C
Microarray Analysis
Genotyping Techniques
Análisis por Micromatrices
Técnicas de Genotipaje
Hepacivirus
Hepatite C
Análise em Microsséries
Técnicas de Genotipagem
dc.subject.es.pt_BR.fl_str_mv Análisis por Micromatrices
Técnicas de Genotipaje
dc.subject.decs.pt_BR.fl_str_mv Hepacivirus
Hepatite C
Análise em Microsséries
Técnicas de Genotipagem
description Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Instituto de Biologia Molecular do Paraná. Curitiba, PR, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-11-14T14:54:49Z
dc.date.available.fl_str_mv 2018-11-14T14:54:49Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DUARTE, César A. B. et al. A novel Hepatitis C Virus genotyping method based on liquid microarray. PLoS ONE, v. 5, n. 9, p. 1-7, 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/30031
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0012822
dc.identifier.eissn.none.fl_str_mv 1932-6203
identifier_str_mv DUARTE, César A. B. et al. A novel Hepatitis C Virus genotyping method based on liquid microarray. PLoS ONE, v. 5, n. 9, p. 1-7, 2010.
1932-6203
10.1371/journal.pone.0012822
url https://www.arca.fiocruz.br/handle/icict/30031
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/30031/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/30031/2/Plosone.00128.pdf
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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