Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C

Detalhes bibliográficos
Autor(a) principal: Nakatani, Sueli M.
Data de Publicação: 2011
Outros Autores: Santos, Carlos A., Riediger, Irina Nastassja, Krieger, Marco Aurélio, Duarte, Cesar A. B., Debur, Maria do Carmo, Carrilho, Flair J., Ono, Suzane K.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/39884
Resumo: Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.
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spelling Nakatani, Sueli M.Santos, Carlos A.Riediger, Irina NastassjaKrieger, Marco AurélioDuarte, Cesar A. B.Debur, Maria do CarmoCarrilho, Flair J.Ono, Suzane K.2020-02-11T19:48:16Z2020-02-11T19:48:16Z2011NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/3988410.1186/1743-422X-8-4591743-422XporBMCHepacivirusHepatitis C virusGenotyping Techniques5' Untranslated RegionsTécnicas de GenotipajeRegiones no Traducidas 5'Técnicas de GenotipagemRegiões 5' não TraduzidasComparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis Cinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Centro de Genomas, São Paulo, São Paulo, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Genotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region. As methods: Plasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis. And as results: comparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B. It has as conclusions that: Sequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/39884/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1743-422X-8-459.pdf1743-422X-8-459.pdfapplication/pdf326298https://www.arca.fiocruz.br/bitstream/icict/39884/2/1743-422X-8-459.pdf45ba59a580d5e1c617925ee36a71f219MD52TEXT1743-422X-8-459.pdf.txt1743-422X-8-459.pdf.txtExtracted texttext/plain24318https://www.arca.fiocruz.br/bitstream/icict/39884/3/1743-422X-8-459.pdf.txtb8565121ae0f77c859fd682f7994ed19MD53icict/398842020-04-09 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dc.title.pt_BR.fl_str_mv Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
title Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
spellingShingle Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
Nakatani, Sueli M.
Hepacivirus
Hepatitis C virus
Genotyping Techniques
5' Untranslated Regions
Técnicas de Genotipaje
Regiones no Traducidas 5'
Técnicas de Genotipagem
Regiões 5' não Traduzidas
title_short Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
title_full Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
title_fullStr Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
title_full_unstemmed Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
title_sort Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
author Nakatani, Sueli M.
author_facet Nakatani, Sueli M.
Santos, Carlos A.
Riediger, Irina Nastassja
Krieger, Marco Aurélio
Duarte, Cesar A. B.
Debur, Maria do Carmo
Carrilho, Flair J.
Ono, Suzane K.
author_role author
author2 Santos, Carlos A.
Riediger, Irina Nastassja
Krieger, Marco Aurélio
Duarte, Cesar A. B.
Debur, Maria do Carmo
Carrilho, Flair J.
Ono, Suzane K.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Nakatani, Sueli M.
Santos, Carlos A.
Riediger, Irina Nastassja
Krieger, Marco Aurélio
Duarte, Cesar A. B.
Debur, Maria do Carmo
Carrilho, Flair J.
Ono, Suzane K.
dc.subject.other.pt_BR.fl_str_mv Hepacivirus
topic Hepacivirus
Hepatitis C virus
Genotyping Techniques
5' Untranslated Regions
Técnicas de Genotipaje
Regiones no Traducidas 5'
Técnicas de Genotipagem
Regiões 5' não Traduzidas
dc.subject.en.pt_BR.fl_str_mv Hepatitis C virus
Genotyping Techniques
5' Untranslated Regions
dc.subject.es.pt_BR.fl_str_mv Técnicas de Genotipaje
Regiones no Traducidas 5'
dc.subject.decs.pt_BR.fl_str_mv Técnicas de Genotipagem
Regiões 5' não Traduzidas
description Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2020-02-11T19:48:16Z
dc.date.available.fl_str_mv 2020-02-11T19:48:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/39884
dc.identifier.issn.pt_BR.fl_str_mv 1743-422X
dc.identifier.doi.none.fl_str_mv 10.1186/1743-422X-8-459
dc.identifier.eissn.none.fl_str_mv 1743-422X
identifier_str_mv NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011.
1743-422X
10.1186/1743-422X-8-459
url https://www.arca.fiocruz.br/handle/icict/39884
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language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC
publisher.none.fl_str_mv BMC
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
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institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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