Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39884 |
Resumo: | Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil. |
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Nakatani, Sueli M.Santos, Carlos A.Riediger, Irina NastassjaKrieger, Marco AurélioDuarte, Cesar A. B.Debur, Maria do CarmoCarrilho, Flair J.Ono, Suzane K.2020-02-11T19:48:16Z2020-02-11T19:48:16Z2011NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/3988410.1186/1743-422X-8-4591743-422XporBMCHepacivirusHepatitis C virusGenotyping Techniques5' Untranslated RegionsTécnicas de GenotipajeRegiones no Traducidas 5'Técnicas de GenotipagemRegiões 5' não TraduzidasComparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis Cinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLaboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Centro de Genomas, São Paulo, São Paulo, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil.Genotyping of hepatitis C virus (HCV) has become an essential tool for prognosis and prediction of treatment duration. The aim of this study was to compare two HCV genotyping methods: reverse hybridization line probe assay (LiPA v.1) and partial sequencing of the NS5B region. As methods: Plasma of 171 patients with chronic hepatitis C were screened using both a commercial method (LiPA HCV Versant, Siemens, Tarrytown, NY, USA) and different primers targeting the NS5B region for PCR amplification and sequencing analysis. And as results: comparison of the HCV genotyping methods showed no difference in the classification at the genotype level. However, a total of 82/171 samples (47.9%) including misclassification, non-subtypable, discrepant and inconclusive results were not classified by LiPA at the subtype level but could be discriminated by NS5B sequencing. Of these samples, 34 samples of genotype 1a and 6 samples of genotype 1b were classified at the subtype level using sequencing of NS5B. It has as conclusions that: Sequence analysis of NS5B for genotyping HCV provides precise genotype and subtype identification and an accurate epidemiological representation of circulating viral strains.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/39884/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINAL1743-422X-8-459.pdf1743-422X-8-459.pdfapplication/pdf326298https://www.arca.fiocruz.br/bitstream/icict/39884/2/1743-422X-8-459.pdf45ba59a580d5e1c617925ee36a71f219MD52TEXT1743-422X-8-459.pdf.txt1743-422X-8-459.pdf.txtExtracted texttext/plain24318https://www.arca.fiocruz.br/bitstream/icict/39884/3/1743-422X-8-459.pdf.txtb8565121ae0f77c859fd682f7994ed19MD53icict/398842020-04-09 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dc.title.pt_BR.fl_str_mv |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
title |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
spellingShingle |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C Nakatani, Sueli M. Hepacivirus Hepatitis C virus Genotyping Techniques 5' Untranslated Regions Técnicas de Genotipaje Regiones no Traducidas 5' Técnicas de Genotipagem Regiões 5' não Traduzidas |
title_short |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
title_full |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
title_fullStr |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
title_full_unstemmed |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
title_sort |
Comparative performance evaluation of hepatitis C virus genotyping based on the 5' untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C |
author |
Nakatani, Sueli M. |
author_facet |
Nakatani, Sueli M. Santos, Carlos A. Riediger, Irina Nastassja Krieger, Marco Aurélio Duarte, Cesar A. B. Debur, Maria do Carmo Carrilho, Flair J. Ono, Suzane K. |
author_role |
author |
author2 |
Santos, Carlos A. Riediger, Irina Nastassja Krieger, Marco Aurélio Duarte, Cesar A. B. Debur, Maria do Carmo Carrilho, Flair J. Ono, Suzane K. |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Nakatani, Sueli M. Santos, Carlos A. Riediger, Irina Nastassja Krieger, Marco Aurélio Duarte, Cesar A. B. Debur, Maria do Carmo Carrilho, Flair J. Ono, Suzane K. |
dc.subject.other.pt_BR.fl_str_mv |
Hepacivirus |
topic |
Hepacivirus Hepatitis C virus Genotyping Techniques 5' Untranslated Regions Técnicas de Genotipaje Regiones no Traducidas 5' Técnicas de Genotipagem Regiões 5' não Traduzidas |
dc.subject.en.pt_BR.fl_str_mv |
Hepatitis C virus Genotyping Techniques 5' Untranslated Regions |
dc.subject.es.pt_BR.fl_str_mv |
Técnicas de Genotipaje Regiones no Traducidas 5' |
dc.subject.decs.pt_BR.fl_str_mv |
Técnicas de Genotipagem Regiões 5' não Traduzidas |
description |
Laboratório Central do Estado do Paraná. São José dos Pinhais, PR, Brasil / Universidade de São Paulo. Faculdade de Medicina. Departamento de Gastroenterologia. São Paulo, SP, Brasil. |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2020-02-11T19:48:16Z |
dc.date.available.fl_str_mv |
2020-02-11T19:48:16Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/39884 |
dc.identifier.issn.pt_BR.fl_str_mv |
1743-422X |
dc.identifier.doi.none.fl_str_mv |
10.1186/1743-422X-8-459 |
dc.identifier.eissn.none.fl_str_mv |
1743-422X |
identifier_str_mv |
NAKATANI, Sueli M. et al. Comparative performance evaluation of hepatitis C virus genotyping based on the 5’ untranslated region versus partial sequencing of the NS5B region of brazilian patients with chronic hepatitis C. Virology Journal, v. 8, n. 459, p. 1-5, 2011. 1743-422X 10.1186/1743-422X-8-459 |
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https://www.arca.fiocruz.br/handle/icict/39884 |
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