Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells

Detalhes bibliográficos
Autor(a) principal: Moura, Andréa A. de
Data de Publicação: 2014
Outros Autores: Kayano, Anderson M., Oliveira, George A., Setúbal, Sulamita S., Ribeiro, João G., Barros, Neuza B., Nicolete, Roberto, Moura, Laura A., Fuly, Andre L., Nomizo, Auro, Silva, Saulo L. da, Fernandes, Carla F. C., Zuliani, Juliana P., Stábeli, Rodrigo G., Soares, Andreimar M., Calderon, Leonardo A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14311
http://dx.doi.org/10.1155/2014/195356
Resumo: Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.
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spelling Moura, Andréa A. deKayano, Anderson M.Oliveira, George A.Setúbal, Sulamita S.Ribeiro, João G.Barros, Neuza B.Nicolete, RobertoMoura, Laura A.Fuly, Andre L.Nomizo, AuroSilva, Saulo L. daFernandes, Carla F. C.Zuliani, Juliana P.Stábeli, Rodrigo G.Soares, Andreimar M.Calderon, Leonardo A.2016-05-17T13:55:08Z2016-05-17T13:55:08Z2014CALDERON, L.A., et al. Purification and biochemical characterization of three myotoxins from Bothrops mattogrossensis snake venom with toxicity against Leishmania and tumor cells. Journal of Biomedicine and Biotechnology (Print), v. 2014, p. 1-13, 2014.https://www.arca.fiocruz.br/handle/icict/14311http://dx.doi.org/10.1155/2014/195356engBioMed Research InternationalPurification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brazil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brazil.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas. Ribeirão Preto, SP, Brazil.Universidade Federal de São João del Rei. Ouro Branco, MG, Brazil.Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil. / Fundação Oswaldo Cruz. Porto Velho, RO, Brazil.Bothrops mattogrossensis snake is widely distributed throughout eastern South America and is responsible for snakebites in this region. This paper reports the purification and biochemical characterization of three new phospholipases A2 (PLA2s), one of which is presumably an enzymatically active Asp49 and two are very likely enzymatically inactive Lys49 PLA2 homologues. The purification was obtained after two chromatographic steps on ion exchange and reverse phase column. The 2D SDS-PAGE analysis revealed that the proteins have pI values around 10, are each made of a single chain, and have molecular masses near 13 kDa, which was confirmed by MALDI-TOF mass spectrometry. The N-terminal similarity analysis of the sequences showed that the proteins are highly homologous with other Lys49 and Asp49 PLA2s from Bothrops species. The PLA2s isolated were named BmatTX-I (Lys49 PLA2-like), BmatTX-II (Lys49 PLA2-like), and BmatTX-III (Asp49 PLA2). The PLA2s induced cytokine release frommouse neutrophils and showed cytotoxicity towards JURKAT (leukemia T) and SK-BR-3 (breast adenocarcinoma) cell lines and promastigote forms of Leishmania amazonensis. The structural and functional elucidation of snake venoms components may contribute to a better understanding of the mechanism of action of these proteins during envenomation and their potential pharmacological and therapeutic applications.Bothrops mattogrossensisSnake VenomLeishmaniaTumor Cellsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/14311/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPurification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells.pdfPurification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells.pdfapplication/pdf2330661https://www.arca.fiocruz.br/bitstream/icict/14311/2/Purification%20and%20Biochemical%20Characterization%20of%20Three%20Myotoxins%20from%20Bothrops%20mattogrossensis%20Snake%20Venom%20with%20Toxicity%20against%20Leishmania%20and%20Tumor%20Cells.pdfd78a494a905fcd4ee4e7b030ef32d219MD52TEXTPurification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells.pdf.txtPurification and Biochemical Characterization of Three Myotoxins from Bothrops mattogrossensis Snake Venom with Toxicity against Leishmania and Tumor Cells.pdf.txtExtracted texttext/plain66031https://www.arca.fiocruz.br/bitstream/icict/14311/3/Purification%20and%20Biochemical%20Characterization%20of%20Three%20Myotoxins%20from%20Bothrops%20mattogrossensis%20Snake%20Venom%20with%20Toxicity%20against%20Leishmania%20and%20Tumor%20Cells.pdf.txtf8b5a161a9436f3bbe478ac1a3b4cd0eMD53icict/143112018-04-02 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dc.title.pt_BR.fl_str_mv Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
title Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
spellingShingle Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
Moura, Andréa A. de
Bothrops mattogrossensis
Snake Venom
Leishmania
Tumor Cells
title_short Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
title_full Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
title_fullStr Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
title_full_unstemmed Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
title_sort Purification and biochemical characterization of three myotoxins from bothrops mattogrossensis snake venom with toxicity against leishmania and tumor cells
author Moura, Andréa A. de
author_facet Moura, Andréa A. de
Kayano, Anderson M.
Oliveira, George A.
Setúbal, Sulamita S.
Ribeiro, João G.
Barros, Neuza B.
Nicolete, Roberto
Moura, Laura A.
Fuly, Andre L.
Nomizo, Auro
Silva, Saulo L. da
Fernandes, Carla F. C.
Zuliani, Juliana P.
Stábeli, Rodrigo G.
Soares, Andreimar M.
Calderon, Leonardo A.
author_role author
author2 Kayano, Anderson M.
Oliveira, George A.
Setúbal, Sulamita S.
Ribeiro, João G.
Barros, Neuza B.
Nicolete, Roberto
Moura, Laura A.
Fuly, Andre L.
Nomizo, Auro
Silva, Saulo L. da
Fernandes, Carla F. C.
Zuliani, Juliana P.
Stábeli, Rodrigo G.
Soares, Andreimar M.
Calderon, Leonardo A.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Moura, Andréa A. de
Kayano, Anderson M.
Oliveira, George A.
Setúbal, Sulamita S.
Ribeiro, João G.
Barros, Neuza B.
Nicolete, Roberto
Moura, Laura A.
Fuly, Andre L.
Nomizo, Auro
Silva, Saulo L. da
Fernandes, Carla F. C.
Zuliani, Juliana P.
Stábeli, Rodrigo G.
Soares, Andreimar M.
Calderon, Leonardo A.
dc.subject.en.pt_BR.fl_str_mv Bothrops mattogrossensis
Snake Venom
Leishmania
Tumor Cells
topic Bothrops mattogrossensis
Snake Venom
Leishmania
Tumor Cells
description Fundação Oswaldo Cruz. Centro de Estudos de Biomoléculas Aplicadas à Saúde. Departamento de Medicina. Núcleo de Saúde. Universidade Federal de Rondônia. Porto Velho, RO, Brazil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-05-17T13:55:08Z
dc.date.available.fl_str_mv 2016-05-17T13:55:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv CALDERON, L.A., et al. Purification and biochemical characterization of three myotoxins from Bothrops mattogrossensis snake venom with toxicity against Leishmania and tumor cells. Journal of Biomedicine and Biotechnology (Print), v. 2014, p. 1-13, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14311
dc.identifier.doi.none.fl_str_mv http://dx.doi.org/10.1155/2014/195356
identifier_str_mv CALDERON, L.A., et al. Purification and biochemical characterization of three myotoxins from Bothrops mattogrossensis snake venom with toxicity against Leishmania and tumor cells. Journal of Biomedicine and Biotechnology (Print), v. 2014, p. 1-13, 2014.
url https://www.arca.fiocruz.br/handle/icict/14311
http://dx.doi.org/10.1155/2014/195356
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BioMed Research International
publisher.none.fl_str_mv BioMed Research International
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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