Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi

Detalhes bibliográficos
Autor(a) principal: Goldenberg, S.
Data de Publicação: 1985
Outros Autores: Salles, J. M., Contreras, V. T., Franco, M. P. Lima, Katzin, A. M., Colli, W., Morel, Carlos Medicis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14685
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Goldenberg, S.Salles, J. M.Contreras, V. T.Franco, M. P. LimaKatzin, A. M.Colli, W.Morel, Carlos Medicis2016-06-28T16:27:41Z2016-07-07T12:16:53Z2016-06-28T16:27:41Z2016-07-07T12:16:53Z1985GOLDENBERG, Samuel; et al. Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi. FEBS Letters, v.180, n.2, p.265-270, Jan. 1985.0014-5793https://www.arca.fiocruz.br/handle/icict/146851873-3468engWileyCharacterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruziinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Niteroi, RJ, Brasil.Universidad de Carabobo. Valencia, Venezuela.Universidade Federal Fluminense. Niteroi, RJ, Brasil.Universidade de São Paulo, Instituto de Química.São Paulo, SP, Brasil.Universidade de São Paulo, Instituto de Química.São Paulo, SP, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.The cell-free translation products of polyribosomal and post-polyribosomal mRNAs from the non-infective epimastigotes and the infective metacyclic trypomastigotes of the parasitic protozoan Trypanosoma cruzi were compared by two-dimensional polyacrylamide gel electrophoresis. The result show that although many polypeptides are conserved, quantitative and qualitative differences are observed between both differentiation stages. The results also indicate the existence of post-polyribosomal mRNAs in equilibrium with polyribosomal counterparts. The immunoprecipitation of the in vitro synthesized polypeptides with chagasic human serum and the serum raised against an 85kDa glycoprotein (PZ-WGA), potentially involved in the process of T. cruzi penetration into mammalian cells, shows that while the chagasic serum recognizes the same 72-kDa, 6%kDa and 46-kDa polypeptides in both differentiation stages, the anti-PZWGA serum immunoprecipitates a single 48-kDa polypeptide from in vitro translation products of metacyclic trypomastigotes.T. cruzimRNACell-free translationSurface antigenChagas diseaseDoença de ChagasTrypanosoma cruziRNA Mensageiroinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTcarlos6_morel_etal_IOC_1985.pdf.txtcarlos6_morel_etal_IOC_1985.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
title Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
spellingShingle Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
Goldenberg, S.
T. cruzi
mRNA
Cell-free translation
Surface antigen
Chagas disease
Doença de Chagas
Trypanosoma cruzi
RNA Mensageiro
title_short Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
title_full Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
title_fullStr Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
title_full_unstemmed Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
title_sort Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi
author Goldenberg, S.
author_facet Goldenberg, S.
Salles, J. M.
Contreras, V. T.
Franco, M. P. Lima
Katzin, A. M.
Colli, W.
Morel, Carlos Medicis
author_role author
author2 Salles, J. M.
Contreras, V. T.
Franco, M. P. Lima
Katzin, A. M.
Colli, W.
Morel, Carlos Medicis
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Goldenberg, S.
Salles, J. M.
Contreras, V. T.
Franco, M. P. Lima
Katzin, A. M.
Colli, W.
Morel, Carlos Medicis
dc.subject.en.pt_BR.fl_str_mv T. cruzi
mRNA
Cell-free translation
Surface antigen
Chagas disease
topic T. cruzi
mRNA
Cell-free translation
Surface antigen
Chagas disease
Doença de Chagas
Trypanosoma cruzi
RNA Mensageiro
dc.subject.decs.pt_BR.fl_str_mv Doença de Chagas
Trypanosoma cruzi
RNA Mensageiro
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1985
dc.date.issued.fl_str_mv 1985
dc.date.accessioned.fl_str_mv 2016-06-28T16:27:41Z
2016-07-07T12:16:53Z
dc.date.available.fl_str_mv 2016-06-28T16:27:41Z
2016-07-07T12:16:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOLDENBERG, Samuel; et al. Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi. FEBS Letters, v.180, n.2, p.265-270, Jan. 1985.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14685
dc.identifier.issn.pt_BR.fl_str_mv 0014-5793
dc.identifier.eissn.pt_BR.fl_str_mv 1873-3468
identifier_str_mv GOLDENBERG, Samuel; et al. Characterization of messenger RNA from epimastigotes and metacyclic trypomastigotes of Trypanosoma cruzi. FEBS Letters, v.180, n.2, p.265-270, Jan. 1985.
0014-5793
1873-3468
url https://www.arca.fiocruz.br/handle/icict/14685
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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