A unique mRNA decapping complex in trypanosomes

Detalhes bibliográficos
Autor(a) principal: Kramer, Susanne
Data de Publicação: 2023
Outros Autores: Karolak, Natalia Katarzyna, Odenwald, Johanna, Gabiatti, Bernardo, Londoño, Paula Andrea Castañeda, Zavrelová, Anna, Freire, Eden Ribeiro, Almeida, Kayo Schemiko, Braune, Silke, Moreira, Claudia, Eder, Amelie, Goos, Carina, Field, Mark, Carrington, Mark, Holetz, Fabiola, Górna, Maria Wiktoria, Zoltner, Martin
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/60752
Resumo: Biocenter. University of Würzburg. Würzburg, Germany.
id CRUZ_a9e9d9ccd5f6d00a4d7055c1de0b007a
oai_identifier_str oai:www.arca.fiocruz.br:icict/60752
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Kramer, SusanneKarolak, Natalia KatarzynaOdenwald, JohannaGabiatti, BernardoLondoño, Paula Andrea CastañedaZavrelová, AnnaFreire, Eden RibeiroAlmeida, Kayo SchemikoBraune, SilkeMoreira, ClaudiaEder, AmelieGoos, CarinaField, MarkCarrington, MarkHoletz, FabiolaGórna, Maria WiktoriaZoltner, Martin2023-10-10T13:30:51Z2023-10-10T13:30:51Z2023KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023.1362-4962https://www.arca.fiocruz.br/handle/icict/60752porOxford University PressmRNATrypanosomeRNA, MessengerARN MensajeroARN messagerTrypanosomaRNA MensageiroAgentes de DecapagemA unique mRNA decapping complex in trypanosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiocenter. University of Würzburg. Würzburg, Germany.Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland. / Nencki Institute of Experimental Biology. Polish Academy of Sciences. Warsaw, Poland.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany.School of Life Sciences. University of Dundee. Dundee, UK. / Institute of Parasitology. Biology Center. Czech Academy of Sciences. České Budějovice, Czech Republic.Department of Biochemistry. University of Cambridge. Cambridge, United Kingdom.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland.Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic.Removal of the mRNA 5' cap primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme Dcp2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5' -3'exoribonuclease Xrn1. Kinetoplastida lack Dcp2 orthologues but instead rely on the ApaH-like phosphatase ALPH1 for decapping. ALPH1 is composed of a catalytic domain flanked by C- and N-terminal extensions. We show that T. brucei ALPH1 is dimeric in vitro and functions within a complex composed of the trypanosome Xrn1 ortholog XRNA and four proteins unique to Kinetoplastida, including two RNA- binding proteins and a CMGC-family protein kinase. All ALPH1-associated proteins share a unique and dynamic localization to a structure at the posterior pole of the cell, anterior to the microtubule plus ends. XRNA affinity capture in T. cruzi recapitulates this interaction network. The ALPH1 N-terminus is not required for viability in culture, but essential for posterior pole localization. The C-terminus, in contrast, is required for localization to all RNA granule types, as well as for dimerization and interactions with XRNA and the CMGC kinase, suggesting possible regulatory mechanisms. Most significantly, the trypanosome decapping complex has a unique composition, differentiating the process from opisthokonts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60752/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgkad497.pdfgkad497.pdfapplication/pdf2763926https://www.arca.fiocruz.br/bitstream/icict/60752/2/gkad497.pdf6d27fa026218325e4ba9eb8fa08ffe34MD52icict/607522023-10-10 10:30:52.375oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-10T13:30:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.en_US.fl_str_mv A unique mRNA decapping complex in trypanosomes
title A unique mRNA decapping complex in trypanosomes
spellingShingle A unique mRNA decapping complex in trypanosomes
Kramer, Susanne
mRNA
Trypanosome
RNA, Messenger
ARN Mensajero
ARN messager
Trypanosoma
RNA Mensageiro
Agentes de Decapagem
title_short A unique mRNA decapping complex in trypanosomes
title_full A unique mRNA decapping complex in trypanosomes
title_fullStr A unique mRNA decapping complex in trypanosomes
title_full_unstemmed A unique mRNA decapping complex in trypanosomes
title_sort A unique mRNA decapping complex in trypanosomes
author Kramer, Susanne
author_facet Kramer, Susanne
Karolak, Natalia Katarzyna
Odenwald, Johanna
Gabiatti, Bernardo
Londoño, Paula Andrea Castañeda
Zavrelová, Anna
Freire, Eden Ribeiro
Almeida, Kayo Schemiko
Braune, Silke
Moreira, Claudia
Eder, Amelie
Goos, Carina
Field, Mark
Carrington, Mark
Holetz, Fabiola
Górna, Maria Wiktoria
Zoltner, Martin
author_role author
author2 Karolak, Natalia Katarzyna
Odenwald, Johanna
Gabiatti, Bernardo
Londoño, Paula Andrea Castañeda
Zavrelová, Anna
Freire, Eden Ribeiro
Almeida, Kayo Schemiko
Braune, Silke
Moreira, Claudia
Eder, Amelie
Goos, Carina
Field, Mark
Carrington, Mark
Holetz, Fabiola
Górna, Maria Wiktoria
Zoltner, Martin
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Kramer, Susanne
Karolak, Natalia Katarzyna
Odenwald, Johanna
Gabiatti, Bernardo
Londoño, Paula Andrea Castañeda
Zavrelová, Anna
Freire, Eden Ribeiro
Almeida, Kayo Schemiko
Braune, Silke
Moreira, Claudia
Eder, Amelie
Goos, Carina
Field, Mark
Carrington, Mark
Holetz, Fabiola
Górna, Maria Wiktoria
Zoltner, Martin
dc.subject.other.en_US.fl_str_mv mRNA
topic mRNA
Trypanosome
RNA, Messenger
ARN Mensajero
ARN messager
Trypanosoma
RNA Mensageiro
Agentes de Decapagem
dc.subject.en.en_US.fl_str_mv Trypanosome
RNA, Messenger
dc.subject.es.en_US.fl_str_mv ARN Mensajero
dc.subject.fr.en_US.fl_str_mv ARN messager
dc.subject.decs.en_US.fl_str_mv Trypanosoma
RNA Mensageiro
Agentes de Decapagem
description Biocenter. University of Würzburg. Würzburg, Germany.
publishDate 2023
dc.date.accessioned.fl_str_mv 2023-10-10T13:30:51Z
dc.date.available.fl_str_mv 2023-10-10T13:30:51Z
dc.date.issued.fl_str_mv 2023
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/60752
dc.identifier.issn.en_US.fl_str_mv 1362-4962
identifier_str_mv KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023.
1362-4962
url https://www.arca.fiocruz.br/handle/icict/60752
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/60752/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/60752/2/gkad497.pdf
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
6d27fa026218325e4ba9eb8fa08ffe34
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009128659877888