A unique mRNA decapping complex in trypanosomes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2023 |
| Outros Autores: | , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/60752 |
Resumo: | Biocenter. University of Würzburg. Würzburg, Germany. |
| id |
CRUZ_a9e9d9ccd5f6d00a4d7055c1de0b007a |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/60752 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Kramer, SusanneKarolak, Natalia KatarzynaOdenwald, JohannaGabiatti, BernardoLondoño, Paula Andrea CastañedaZavrelová, AnnaFreire, Eden RibeiroAlmeida, Kayo SchemikoBraune, SilkeMoreira, ClaudiaEder, AmelieGoos, CarinaField, MarkCarrington, MarkHoletz, FabiolaGórna, Maria WiktoriaZoltner, Martin2023-10-10T13:30:51Z2023-10-10T13:30:51Z2023KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023.1362-4962https://www.arca.fiocruz.br/handle/icict/60752porOxford University PressmRNATrypanosomeRNA, MessengerARN MensajeroARN messagerTrypanosomaRNA MensageiroAgentes de DecapagemA unique mRNA decapping complex in trypanosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBiocenter. University of Würzburg. Würzburg, Germany.Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland. / Nencki Institute of Experimental Biology. Polish Academy of Sciences. Warsaw, Poland.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany. / Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biocenter. University of Würzburg. Würzburg, Germany.Biocenter. University of Würzburg. Würzburg, Germany.School of Life Sciences. University of Dundee. Dundee, UK. / Institute of Parasitology. Biology Center. Czech Academy of Sciences. České Budějovice, Czech Republic.Department of Biochemistry. University of Cambridge. Cambridge, United Kingdom.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Biological and Chemical Research Centre. Department of Chemistry. University of Warsaw. Warsaw, Poland.Department of Parasitology. Faculty of Science. Charles University in Prague. Biocev, Vestec, Czech Republic.Removal of the mRNA 5' cap primes transcripts for degradation and is central for regulating gene expression in eukaryotes. The canonical decapping enzyme Dcp2 is stringently controlled by assembly into a dynamic multi-protein complex together with the 5' -3'exoribonuclease Xrn1. Kinetoplastida lack Dcp2 orthologues but instead rely on the ApaH-like phosphatase ALPH1 for decapping. ALPH1 is composed of a catalytic domain flanked by C- and N-terminal extensions. We show that T. brucei ALPH1 is dimeric in vitro and functions within a complex composed of the trypanosome Xrn1 ortholog XRNA and four proteins unique to Kinetoplastida, including two RNA- binding proteins and a CMGC-family protein kinase. All ALPH1-associated proteins share a unique and dynamic localization to a structure at the posterior pole of the cell, anterior to the microtubule plus ends. XRNA affinity capture in T. cruzi recapitulates this interaction network. The ALPH1 N-terminus is not required for viability in culture, but essential for posterior pole localization. The C-terminus, in contrast, is required for localization to all RNA granule types, as well as for dimerization and interactions with XRNA and the CMGC kinase, suggesting possible regulatory mechanisms. Most significantly, the trypanosome decapping complex has a unique composition, differentiating the process from opisthokonts.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/60752/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALgkad497.pdfgkad497.pdfapplication/pdf2763926https://www.arca.fiocruz.br/bitstream/icict/60752/2/gkad497.pdf6d27fa026218325e4ba9eb8fa08ffe34MD52icict/607522023-10-10 10:30:52.375oai:www.arca.fiocruz.br:icict/60752Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-10-10T13:30:52Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
A unique mRNA decapping complex in trypanosomes |
| title |
A unique mRNA decapping complex in trypanosomes |
| spellingShingle |
A unique mRNA decapping complex in trypanosomes Kramer, Susanne mRNA Trypanosome RNA, Messenger ARN Mensajero ARN messager Trypanosoma RNA Mensageiro Agentes de Decapagem |
| title_short |
A unique mRNA decapping complex in trypanosomes |
| title_full |
A unique mRNA decapping complex in trypanosomes |
| title_fullStr |
A unique mRNA decapping complex in trypanosomes |
| title_full_unstemmed |
A unique mRNA decapping complex in trypanosomes |
| title_sort |
A unique mRNA decapping complex in trypanosomes |
| author |
Kramer, Susanne |
| author_facet |
Kramer, Susanne Karolak, Natalia Katarzyna Odenwald, Johanna Gabiatti, Bernardo Londoño, Paula Andrea Castañeda Zavrelová, Anna Freire, Eden Ribeiro Almeida, Kayo Schemiko Braune, Silke Moreira, Claudia Eder, Amelie Goos, Carina Field, Mark Carrington, Mark Holetz, Fabiola Górna, Maria Wiktoria Zoltner, Martin |
| author_role |
author |
| author2 |
Karolak, Natalia Katarzyna Odenwald, Johanna Gabiatti, Bernardo Londoño, Paula Andrea Castañeda Zavrelová, Anna Freire, Eden Ribeiro Almeida, Kayo Schemiko Braune, Silke Moreira, Claudia Eder, Amelie Goos, Carina Field, Mark Carrington, Mark Holetz, Fabiola Górna, Maria Wiktoria Zoltner, Martin |
| author2_role |
author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Kramer, Susanne Karolak, Natalia Katarzyna Odenwald, Johanna Gabiatti, Bernardo Londoño, Paula Andrea Castañeda Zavrelová, Anna Freire, Eden Ribeiro Almeida, Kayo Schemiko Braune, Silke Moreira, Claudia Eder, Amelie Goos, Carina Field, Mark Carrington, Mark Holetz, Fabiola Górna, Maria Wiktoria Zoltner, Martin |
| dc.subject.other.en_US.fl_str_mv |
mRNA |
| topic |
mRNA Trypanosome RNA, Messenger ARN Mensajero ARN messager Trypanosoma RNA Mensageiro Agentes de Decapagem |
| dc.subject.en.en_US.fl_str_mv |
Trypanosome RNA, Messenger |
| dc.subject.es.en_US.fl_str_mv |
ARN Mensajero |
| dc.subject.fr.en_US.fl_str_mv |
ARN messager |
| dc.subject.decs.en_US.fl_str_mv |
Trypanosoma RNA Mensageiro Agentes de Decapagem |
| description |
Biocenter. University of Würzburg. Würzburg, Germany. |
| publishDate |
2023 |
| dc.date.accessioned.fl_str_mv |
2023-10-10T13:30:51Z |
| dc.date.available.fl_str_mv |
2023-10-10T13:30:51Z |
| dc.date.issued.fl_str_mv |
2023 |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/60752 |
| dc.identifier.issn.en_US.fl_str_mv |
1362-4962 |
| identifier_str_mv |
KRAMER, Susanne et al. A unique mRNA decapping complex in trypanosomes. Nucleic Acids Research. v. 51, n. 14, p. 7520-7540, 2023. 1362-4962 |
| url |
https://www.arca.fiocruz.br/handle/icict/60752 |
| dc.language.iso.fl_str_mv |
por |
| language |
por |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Oxford University Press |
| publisher.none.fl_str_mv |
Oxford University Press |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/60752/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/60752/2/gkad497.pdf |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 6d27fa026218325e4ba9eb8fa08ffe34 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324911222030336 |