The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases

Detalhes bibliográficos
Autor(a) principal: Tuyama, Mary
Data de Publicação: 2008
Outros Autores: Boente, Renata F., Rebelo, Maria C., Igreja, Ricardo P., Barroso, David E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/28170
Resumo: Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.
id CRUZ_22cde635e2fe90d938e0964bfa202788
oai_identifier_str oai:www.arca.fiocruz.br:icict/28170
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Tuyama, MaryBoente, Renata F.Rebelo, Maria C.Igreja, Ricardo P.Barroso, David E.2018-08-16T12:23:27Z2018-08-16T12:23:27Z2008TUYAMA, Mary; et al. The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases. Mem Inst Oswaldo Cruz, vol.103, n.2, p.138-142, Mar. 2008.0074-0276https://www.arca.fiocruz.br/handle/icict/281701678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzLíquido CefalorraquidianoMeningiteStreptococcus pneumoniaeNeisseria meningitidisHaemophilus influenzaeReação em Cadeia da PolimerasemeningitisPCRStreptococcus pneumoniaeNeisseria meningitidisHaemophilus influenzaecerebrospinal fluidThe utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis casesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Grupo de Doenças Virais, Rickettsioses e Febres Hemorrágicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Enterovírus. Grupo de Doenças Virais, Rickettsioses e Febres Hemorrágicas. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ. Brasil.Most patients with acute suppurative meningitis are otherwise healthy individuals with regard to immune mechanisms against invasive bacterial disease. This medical emergency is among the most dramatic and potentially ravaging diseases that affect humans, particularly young children. The illness often strikes suddenly, and can either result in death or leave the survivors with significant neurological dysfunctions. The demonstration of a bacterial aetiology is necessary for decisions regarding treatment and prophylaxis. Conventional bacteriological methods frequently fail to identify an agent, as a result of administration of antibiotics or delayed lumbar punctures. We investigated the major aetiologic sources of unspecified bacterial meningitis cases (G00.9, ISCD-10) by polymerase chain reaction (PCR)-based identification of Neisseria meningitidis (crgA), Streptococcus pneumoniae (ply) and Haemophilus influenzae (bexA) in cerebrospinal fluid samples. The multiplex PCR detected N. meningitidis in 92%, S. pneumoniae in 4% and H. influenzae in 1% of the 192 clinical samples assayed; 3% were negative for all three DNA targets. Bacterial DNA detection was found to be a valuable adjunct to enhance bacterial meningitis surveillance when the yield of specimens by culture is reduced. The implementation of PCR assays as a diagnostic procedure in Public Health Laboratories is perceived to be a significant advance in the investigation of bacterial meningitis.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28170/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALmary_tuyama_etal_IOC_2008.pdfmary_tuyama_etal_IOC_2008.pdfapplication/pdf480890https://www.arca.fiocruz.br/bitstream/icict/28170/2/mary_tuyama_etal_IOC_2008.pdf97426db97e3a238d2e2b66be05b2e420MD52TEXTmary_tuyama_etal_IOC_2008.pdf.txtmary_tuyama_etal_IOC_2008.pdf.txtExtracted texttext/plain28100https://www.arca.fiocruz.br/bitstream/icict/28170/3/mary_tuyama_etal_IOC_2008.pdf.txt5d71c6f1b73e6c05e69119ebbf688456MD53icict/281702018-08-17 02:00:57.203oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-08-17T05:00:57Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
title The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
spellingShingle The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
Tuyama, Mary
Líquido Cefalorraquidiano
Meningite
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
Reação em Cadeia da Polimerase
meningitis
PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
cerebrospinal fluid
title_short The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
title_full The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
title_fullStr The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
title_full_unstemmed The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
title_sort The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases
author Tuyama, Mary
author_facet Tuyama, Mary
Boente, Renata F.
Rebelo, Maria C.
Igreja, Ricardo P.
Barroso, David E.
author_role author
author2 Boente, Renata F.
Rebelo, Maria C.
Igreja, Ricardo P.
Barroso, David E.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Tuyama, Mary
Boente, Renata F.
Rebelo, Maria C.
Igreja, Ricardo P.
Barroso, David E.
dc.subject.other.pt_BR.fl_str_mv Líquido Cefalorraquidiano
Meningite
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
Reação em Cadeia da Polimerase
topic Líquido Cefalorraquidiano
Meningite
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
Reação em Cadeia da Polimerase
meningitis
PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
cerebrospinal fluid
dc.subject.en.pt_BR.fl_str_mv meningitis
PCR
Streptococcus pneumoniae
Neisseria meningitidis
Haemophilus influenzae
cerebrospinal fluid
description Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Serviço de Doenças Infecciosas e Parasitárias. Rio de Janeiro, RJ, Brasil.
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2018-08-16T12:23:27Z
dc.date.available.fl_str_mv 2018-08-16T12:23:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv TUYAMA, Mary; et al. The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases. Mem Inst Oswaldo Cruz, vol.103, n.2, p.138-142, Mar. 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/28170
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv TUYAMA, Mary; et al. The utility of the polymerase chain reaction assay for aetiologic definition of unspecified bacterial meningitis cases. Mem Inst Oswaldo Cruz, vol.103, n.2, p.138-142, Mar. 2008.
0074-0276
1678-8060
url https://www.arca.fiocruz.br/handle/icict/28170
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/28170/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/28170/2/mary_tuyama_etal_IOC_2008.pdf
https://www.arca.fiocruz.br/bitstream/icict/28170/3/mary_tuyama_etal_IOC_2008.pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
97426db97e3a238d2e2b66be05b2e420
5d71c6f1b73e6c05e69119ebbf688456
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324638061690880

Registros relacionados