Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis

Detalhes bibliográficos
Autor(a) principal: Wu, Henry M.
Data de Publicação: 2013
Outros Autores: Cordeiro, Soraia Machado, Harcourt, Brian H., Carvalho, Maria da Gloria S., Azevedo, Jailton, Oliveira, Tainara Q., Leite, Mariela C., Salgado, Katia, Reis, Mitermayer Galvão dos, Plikaytis, Brian D., Clark, Thomas A., Mayer, Leonard W., Ko, Albert I, Martin, Stacey W., Reis, Joice N.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13403
Resumo: p. 1-10
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spelling Wu, Henry M.Cordeiro, Soraia MachadoHarcourt, Brian H.Carvalho, Maria da Gloria S.Azevedo, JailtonOliveira, Tainara Q.Leite, Mariela C.Salgado, KatiaReis, Mitermayer Galvão dosPlikaytis, Brian D.Clark, Thomas A.Mayer, Leonard W.Ko, Albert IMartin, Stacey W.Reis, Joice N.Wu, Henry M.Cordeiro, Soraia MachadoHarcourt, Brian H.Carvalho, Maria da Gloria S.Azevedo, JailtonOliveira, Tainara Q.Leite, Mariela C.Salgado, KatiaReis, Mitermayer Galvão dosPlikaytis, Brian D.Clark, Thomas A.Mayer, Leonard W.Ko, Albert IMartin, Stacey W.Reis, Joice N.2013-10-31T17:43:19Z2013-10-31T17:43:19Z20131471-2334http://repositorio.ufba.br/ri/handle/ri/13403v. 13, n. 26p. 1-10Background: Although cerebrospinal fluid (CSF) culture is the diagnostic reference standard for bacterial meningitis, its sensitivity is limited, particularly when antibiotics were previously administered. CSF Gram staining and real-time PCR are theoretically less affected by antibiotics; however, it is difficult to evaluate these tests with an imperfect reference standard. Methods and findings: CSF from patients with suspected meningitis from Salvador, Brazil were tested with culture, Gram stain, and real-time PCR using S. pneumoniae, N. meningitidis, and H. influenzae specific primers and probes. An antibiotic detection disk bioassay was used to test for the presence of antibiotic activity in CSF. The diagnostic accuracy of tests were evaluated using multiple methods, including direct evaluation of Gram stain and real-time PCR against CSF culture, evaluation of real-time PCR against a composite reference standard, and latent class analysis modeling to evaluate all three tests simultaneously. Results: Among 451 CSF specimens, 80 (17.7%) had culture isolation of one of the three pathogens (40 S. pneumoniae, 36 N. meningitidis, and 4 H. influenzae), and 113 (25.1%) were real-time PCR positive (51 S. pneumoniae, 57 N. meningitidis, and 5 H. influenzae). Compared to culture, real-time PCR sensitivity and specificity were 95.0% and 90.0%, respectively. In a latent class analysis model, the sensitivity and specificity estimates were: culture, 81.3% and 99.7%; Gram stain, 98.2% and 98.7%; and real-time PCR, 95.7% and 94.3%, respectively. Gram stain and real-time PCR sensitivity did not change significantly when there was antibiotic activity in the CSF. Conclusion: Real-time PCR and Gram stain were highly accurate in diagnosing meningitis caused by S. pneumoniae, N. meningitidis, and H. influenzae, though there were few cases of H. influenzae. Furthermore, real-time PCR and Gram staining were less affected by antibiotic presence and might be useful when antibiotics were previously administered. Gram staining, which is inexpensive and commonly available, should be encouraged in all clinical settings.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-07-08T13:05:43Z No. of bitstreams: 1 444444444444.pdf: 281795 bytes, checksum: 594f3384ea132f663567fb63c1ab1d4e (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-31T17:43:19Z (GMT) No. of bitstreams: 1 444444444444.pdf: 281795 bytes, checksum: 594f3384ea132f663567fb63c1ab1d4e (MD5)Made available in DSpace on 2013-10-31T17:43:19Z (GMT). No. of bitstreams: 1 444444444444.pdf: 281795 bytes, checksum: 594f3384ea132f663567fb63c1ab1d4e (MD5) Previous issue date: 2013SalvadorBMC Infectious Diseases10.1186/1471-2334-13-26reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBABacterial meningitisDiagnostic test evaluationReal-time PCRStreptococcus pneumoniae Neisseria meningitidisHaemophilus influenzaeAccuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosisBMC Infectious Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL444444444444.pdf444444444444.pdfapplication/pdf281795https://repositorio.ufba.br/bitstream/ri/13403/1/444444444444.pdf594f3384ea132f663567fb63c1ab1d4eMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13403/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT444444444444.pdf.txt444444444444.pdf.txtExtracted texttext/plain48068https://repositorio.ufba.br/bitstream/ri/13403/3/444444444444.pdf.txta0357824d4ef0efc9149ddda46d36fd6MD53ri/134032022-07-05 14:03:14.975oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
dc.title.alternative.pt_BR.fl_str_mv BMC Infectious Diseases
title Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
spellingShingle Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
Wu, Henry M.
Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae Neisseria meningitidis
Haemophilus influenzae
title_short Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_full Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_fullStr Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_full_unstemmed Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
title_sort Accuracy of real-time PCR, Gram stain and culture for Streptococcus pneumoniae, Neisseria meningitidis and Haemophilus influenzae meningitis diagnosis
author Wu, Henry M.
author_facet Wu, Henry M.
Cordeiro, Soraia Machado
Harcourt, Brian H.
Carvalho, Maria da Gloria S.
Azevedo, Jailton
Oliveira, Tainara Q.
Leite, Mariela C.
Salgado, Katia
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A.
Mayer, Leonard W.
Ko, Albert I
Martin, Stacey W.
Reis, Joice N.
author_role author
author2 Cordeiro, Soraia Machado
Harcourt, Brian H.
Carvalho, Maria da Gloria S.
Azevedo, Jailton
Oliveira, Tainara Q.
Leite, Mariela C.
Salgado, Katia
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A.
Mayer, Leonard W.
Ko, Albert I
Martin, Stacey W.
Reis, Joice N.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wu, Henry M.
Cordeiro, Soraia Machado
Harcourt, Brian H.
Carvalho, Maria da Gloria S.
Azevedo, Jailton
Oliveira, Tainara Q.
Leite, Mariela C.
Salgado, Katia
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A.
Mayer, Leonard W.
Ko, Albert I
Martin, Stacey W.
Reis, Joice N.
Wu, Henry M.
Cordeiro, Soraia Machado
Harcourt, Brian H.
Carvalho, Maria da Gloria S.
Azevedo, Jailton
Oliveira, Tainara Q.
Leite, Mariela C.
Salgado, Katia
Reis, Mitermayer Galvão dos
Plikaytis, Brian D.
Clark, Thomas A.
Mayer, Leonard W.
Ko, Albert I
Martin, Stacey W.
Reis, Joice N.
dc.subject.por.fl_str_mv Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae Neisseria meningitidis
Haemophilus influenzae
topic Bacterial meningitis
Diagnostic test evaluation
Real-time PCR
Streptococcus pneumoniae Neisseria meningitidis
Haemophilus influenzae
description p. 1-10
publishDate 2013
dc.date.accessioned.fl_str_mv 2013-10-31T17:43:19Z
dc.date.available.fl_str_mv 2013-10-31T17:43:19Z
dc.date.issued.fl_str_mv 2013
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13403
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.number.pt_BR.fl_str_mv v. 13, n. 26
identifier_str_mv 1471-2334
v. 13, n. 26
url http://repositorio.ufba.br/ri/handle/ri/13403
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv BMC Infectious Diseases
publisher.none.fl_str_mv BMC Infectious Diseases
dc.source.pt_BR.fl_str_mv 10.1186/1471-2334-13-26
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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