Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus

Detalhes bibliográficos
Autor(a) principal: Soares, Alexandra M. S.
Data de Publicação: 2018
Outros Autores: Oliveira, Jose T. A., Rocha, Cláudia Q., Ferreira, André T. S., Perales, Jonas, Zanatta, Ana Caroline, Vilegas, Wagner, Silva, Carolina R., Costa Junior, Livio M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31563
Resumo: Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
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spelling Soares, Alexandra M. S.Oliveira, Jose T. A.Rocha, Cláudia Q.Ferreira, André T. S.Perales, JonasZanatta, Ana CarolineVilegas, WagnerSilva, Carolina R.Costa Junior, Livio M.2019-02-12T12:18:15Z2019-02-12T12:18:15Z2018SOARES, Alexandra M. S. et al. Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus. Plos One, v.13, n.7, e0200848, 12p, July 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3156310.1371/journal.pone.0200848engPublic Library of ScienceMyracrodruon urundeuvaProteomaAtividade anti-helmínticaHaemonchus contortusSementesMyracrodruon urundeuvaProteomeAnthelmintic activityHaemonchus contortusSeedsMyracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.Universidade Federal do Ceará. Laboratório de Proteínas de Defesa Vegetal. Campus do Pici. Fortaleza, CE, Brasil.Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Departamento de Química. Laboratório de Estudos Avançados em Fitomedicamentos. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Instituto de Biociências. Bioprospecção. Laboratório de Produtos Naturais. Campus Costeiro de São Vicente. São Vicente, SP, Brasil.Instituto de Biociências. Bioprospecção. Laboratório de Produtos Naturais. Campus Costeiro de São Vicente. São Vicente, SP, Brasil.Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitas. São Luís, MA, Brasil.Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitas. São Luís, MA, Brasil.Seed exudates are plant-derived natural bioactive compounds consisting of a complex mixture of organic and inorganic molecules. Plant seed exudates have been poorly studied against parasite nematodes. This study was undertaken to identify proteins in the Myracrodruon urundeuva seed exudates and to assess the anthelmintic activity against Haemonchus contortus, an important parasite of small ruminants. M. urundeuva seed exudates (SEX) was obtained after immersion of seeds in sodium acetate buffer. SEX was fractionated with ammonium sulfate at 0-90% concentration to generate the ressuspended pellet (SEXF1) and the supernatant (SEXF2). SEX, SEXF1, and SEXF2 were exhaustively dialyzed against distilled water (cut-off: 12 kDa) and the protein contents determined. Mass spectrometry analyses of SEX, SEXF1, and SEXF2 were done to identify proteins and secondary metabolites. The seed exudates contained protease, protease inhibitor, peptidase, chitinase, and lipases as well as the low molecular weight secondary compounds ellagic acid and quercetin rhamnoside. SEX inhibited H. contortus larval development (LDA) (IC50 = 0.29 mg mL-1), but did not affect larval exsheathment (LEIA). On the other hand, although SEXF1 and SEXF2 inhibited H. contortus LEIA (IC50 = 1.04 and 0.93 mg mL-1, respectively), they showed even greater inhibition efficiency of H. contortus larval development (IC50 = 0.29 and 0.42 mg mL-1, respectively). To the best of our knowledge, this study is the first to show the anthelmintic activity of plant exudates against a gastrointestinal nematode. Moreover, it suggests the potential of exuded proteins as candidates to negatively interfere with H. contortus life cycle.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31563/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALandreteixeira_jonasperales_etal_IOC_2018.pdfandreteixeira_jonasperales_etal_IOC_2018.pdfapplication/pdf2205193https://www.arca.fiocruz.br/bitstream/icict/31563/2/andreteixeira_jonasperales_etal_IOC_2018.pdf5afbc86b61d4f64cce1747344eba2377MD52TEXTandreteixeira_jonasperales_etal_IOC_2018.pdf.txtandreteixeira_jonasperales_etal_IOC_2018.pdf.txtExtracted texttext/plain41494https://www.arca.fiocruz.br/bitstream/icict/31563/3/andreteixeira_jonasperales_etal_IOC_2018.pdf.txt4dd982e80f1548855832dd8fe6ae5c0bMD53icict/315632019-02-13 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dc.title.pt_BR.fl_str_mv Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
title Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
spellingShingle Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
Soares, Alexandra M. S.
Myracrodruon urundeuva
Proteoma
Atividade anti-helmíntica
Haemonchus contortus
Sementes
Myracrodruon urundeuva
Proteome
Anthelmintic activity
Haemonchus contortus
Seeds
title_short Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
title_full Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
title_fullStr Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
title_full_unstemmed Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
title_sort Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus
author Soares, Alexandra M. S.
author_facet Soares, Alexandra M. S.
Oliveira, Jose T. A.
Rocha, Cláudia Q.
Ferreira, André T. S.
Perales, Jonas
Zanatta, Ana Caroline
Vilegas, Wagner
Silva, Carolina R.
Costa Junior, Livio M.
author_role author
author2 Oliveira, Jose T. A.
Rocha, Cláudia Q.
Ferreira, André T. S.
Perales, Jonas
Zanatta, Ana Caroline
Vilegas, Wagner
Silva, Carolina R.
Costa Junior, Livio M.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Soares, Alexandra M. S.
Oliveira, Jose T. A.
Rocha, Cláudia Q.
Ferreira, André T. S.
Perales, Jonas
Zanatta, Ana Caroline
Vilegas, Wagner
Silva, Carolina R.
Costa Junior, Livio M.
dc.subject.other.pt_BR.fl_str_mv Myracrodruon urundeuva
Proteoma
Atividade anti-helmíntica
Haemonchus contortus
Sementes
topic Myracrodruon urundeuva
Proteoma
Atividade anti-helmíntica
Haemonchus contortus
Sementes
Myracrodruon urundeuva
Proteome
Anthelmintic activity
Haemonchus contortus
Seeds
dc.subject.en.pt_BR.fl_str_mv Myracrodruon urundeuva
Proteome
Anthelmintic activity
Haemonchus contortus
Seeds
description Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-12T12:18:15Z
dc.date.available.fl_str_mv 2019-02-12T12:18:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SOARES, Alexandra M. S. et al. Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus. Plos One, v.13, n.7, e0200848, 12p, July 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31563
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0200848
identifier_str_mv SOARES, Alexandra M. S. et al. Myracrodruon urundeuva seed exudates proteome and anthelmintic activity against Haemonchus contortus. Plos One, v.13, n.7, e0200848, 12p, July 2018.
1932-6203
10.1371/journal.pone.0200848
url https://www.arca.fiocruz.br/handle/icict/31563
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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