In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus

Detalhes bibliográficos
Autor(a) principal: Araújo, Sandra Alves
Data de Publicação: 2017
Outros Autores: Soares, Alexandra Martins dos Santos, Silva, Carolina Rocha, Almeida Júnior, Eduardo Bezerra, Rocha, Cláudia Quintino, Ferreira, André Teixeira da Silva, Perales, Jonas, Costa Júnior, Livio M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/24850
Resumo: Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
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spelling Araújo, Sandra AlvesSoares, Alexandra Martins dos SantosSilva, Carolina RochaAlmeida Júnior, Eduardo BezerraRocha, Cláudia QuintinoFerreira, André Teixeira da SilvaPerales, JonasCosta Júnior, Livio M.2018-02-15T17:05:52Z2018-02-15T17:05:52Z2017ARAÚJO, Sandra Alves; et al. In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus. PLos One, v.12, n.12, e0189803, Dec. 2017.1932-6203https://www.arca.fiocruz.br/handle/icict/2485010.1371/journal.pone.0189803engPublic Library of Scienceefeitos anti-helmínticosSpigelia anthelmiaHaemonchus contortusSpigelia anthelmia proteinHaemonchus contortusanthelmintic effectsIn vitroIn vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitos. São Luis, MA, BrasilUniversidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Departamento de Química. Laboratório de Estudos Avançados em Fitomedicina. São Luís, MA, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Toxinologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Maranhão. Centro de Ciências Biológicas e da Saúde. Departamento de Patologia. Laboratório de Controle de Parasitos. São Luis, MA, BrasilGastrointestinal nematodes are a significant concern for animal health and well-being, and anthelmintic treatment is mainly performed through the use of chemical products. However, bioactive compounds produced by plants have shown promise for development as novel anthelmintics. The aim of this study is to assess the anthelmintic activity of protein fractions from Spigelia anthelmia on the gastrointestinal nematode Haemonchus contortus. Plant parts were separated into leaves, stems and roots, washed with distilled water, freeze-dried and ground into a fine powder. Protein extraction was performed with sodium phosphate buffer (75 mM, pH 7.0). The extract was fractionated using ammonium sulfate (0-90%) and extensively dialyzed. The resulting fractions were named LPF (leaf protein fraction), SPF (stem protein fraction) and RPF (root protein fraction), and the protein contents and activities of the fractions were analyzed. H. contortus egg hatching (EHA), larval exsheathment inhibition (LEIA) and larval migration inhibition (LMIA) assays were performed. Proteomic analysis was conducted, and high-performance liquid chromatography (HPLC) chromatographic profiles of the fractions were established to identify proteins and possible secondary metabolites. S. anthelmia fractions inhibited H. contortus egg hatching, with LPF having the most potent effects (EC50 0.17 mg mL-1). During LEIA, SPF presented greater efficiency than the other fractions (EC50 0.25 mg mL-1). According to LMIA, the fractions from roots, stems and leaves also reduced the number of larvae, with EC50 values of 0.11, 0.14 and 0.21 mg mL-1, respectively. Protein analysis indicated the presence of plant defense proteins in the S. anthelmia fractions, including protease, protease inhibitor, chitinase and others. Conversely, secondary metabolites were absent in the S. anthemia fractions. These results suggest that S. anthelmia proteins are promising for the control of the gastrointestinal nematode H. contortus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/24850/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALjonas_perales_etal_IOC_2017.pdfjonas_perales_etal_IOC_2017.pdfapplication/pdf1369524https://www.arca.fiocruz.br/bitstream/icict/24850/2/jonas_perales_etal_IOC_2017.pdf3fbcbf59d8fec057933f94c614583de9MD52TEXTjonas_perales_etal_IOC_2017.pdf.txtjonas_perales_etal_IOC_2017.pdf.txtExtracted texttext/plain40930https://www.arca.fiocruz.br/bitstream/icict/24850/3/jonas_perales_etal_IOC_2017.pdf.txt7156659f4a81179e06232de2f6338cf2MD53icict/248502023-09-05 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dc.title.pt_BR.fl_str_mv In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
title In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
spellingShingle In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
Araújo, Sandra Alves
efeitos anti-helmínticos
Spigelia anthelmia
Haemonchus contortus
Spigelia anthelmia protein
Haemonchus contortus
anthelmintic effects
In vitro
title_short In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
title_full In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
title_fullStr In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
title_full_unstemmed In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
title_sort In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus
author Araújo, Sandra Alves
author_facet Araújo, Sandra Alves
Soares, Alexandra Martins dos Santos
Silva, Carolina Rocha
Almeida Júnior, Eduardo Bezerra
Rocha, Cláudia Quintino
Ferreira, André Teixeira da Silva
Perales, Jonas
Costa Júnior, Livio M.
author_role author
author2 Soares, Alexandra Martins dos Santos
Silva, Carolina Rocha
Almeida Júnior, Eduardo Bezerra
Rocha, Cláudia Quintino
Ferreira, André Teixeira da Silva
Perales, Jonas
Costa Júnior, Livio M.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Araújo, Sandra Alves
Soares, Alexandra Martins dos Santos
Silva, Carolina Rocha
Almeida Júnior, Eduardo Bezerra
Rocha, Cláudia Quintino
Ferreira, André Teixeira da Silva
Perales, Jonas
Costa Júnior, Livio M.
dc.subject.other.pt_BR.fl_str_mv efeitos anti-helmínticos
Spigelia anthelmia
Haemonchus contortus
topic efeitos anti-helmínticos
Spigelia anthelmia
Haemonchus contortus
Spigelia anthelmia protein
Haemonchus contortus
anthelmintic effects
In vitro
dc.subject.en.pt_BR.fl_str_mv Spigelia anthelmia protein
Haemonchus contortus
anthelmintic effects
In vitro
description Universidade Federal do Maranhão. Centro de Ciências Exatas e Tecnologia. Curso de Engenharia Química. Laboratório de Bioquímica Vegetal. São Luiz, MA, Brasil.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-02-15T17:05:52Z
dc.date.available.fl_str_mv 2018-02-15T17:05:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ARAÚJO, Sandra Alves; et al. In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus. PLos One, v.12, n.12, e0189803, Dec. 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/24850
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0189803
identifier_str_mv ARAÚJO, Sandra Alves; et al. In vitro anthelmintic effects of Spigelia anthelmia protein fractions against Haemonchus contortus. PLos One, v.12, n.12, e0189803, Dec. 2017.
1932-6203
10.1371/journal.pone.0189803
url https://www.arca.fiocruz.br/handle/icict/24850
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/24850/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/24850/2/jonas_perales_etal_IOC_2017.pdf
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