Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon

Detalhes bibliográficos
Autor(a) principal: Chagas, Andrezza Campos
Data de Publicação: 2010
Outros Autores: Medeiros, Jansen Fernandes, Astolfi Filho, Spartaco, Py-Daniel, Victor
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/27684
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.
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spelling Chagas, Andrezza CamposMedeiros, Jansen FernandesAstolfi Filho, SpartacoPy-Daniel, Victor2018-07-27T12:57:01Z2018-07-27T12:57:01Z2010CHAGAS, Andrezza Campos et al. Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 2, p.174-178, Feb. 2010.0074-0276https://www.arca.fiocruz.br/handle/icict/2768410.1590/S0074-02762010000200011engFundação Oswaldo Cruz.Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Coordenação de Pesquisas em Ciências da Saúde. Porto Velho, RO, Brasil.Universidade Federal do Amazonas. Centro de Apoio Multidisciplinar. Laboratório de Tecnologias de DNA. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Núcleo de Ciências Humanas e Sociais. Manaus, AM, Brasil.In this study, anticoagulant activity was detected in salivary gland homogenates (SGHs) of Thyrsopelma guianense (Diptera: Simuliidae). The SGH yielded 1.07 μg ± 0.03 (n = 15) of total soluble protein per pair of glands. In addition, following SDS-PAGE (12.5% gel) and silver nitrate staining, 12 polypeptides with molecular weights ranging from 14-69 kDa were detected in all physiological ages analyzed (12 h, 24 h, 48 h and 72 h following emergence). Coagulation bioassays showed that the SGHs had activities that interacted at all levels of coagulation (the intrinsic, extrinsic and common pathways), by extending the plasma recalcification time, prothrombin time, thrombin time. This is the first report on the activity of salivary gland proteins from the main vector of onchocerciasis in Brazil. We also suggest detailed studies on the morphology and function of the salivary glands in order to understand the role of these proteins in host/vector interactions.Moscas negrasGlândulas salivaresAnticoagulanteOncocercoseBlack fliesSalivary glandsAnticoagulantOnchocerciasisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/27684/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAnticoagulant activity in salivary.pdfAnticoagulant activity in salivary.pdfapplication/pdf359336https://www.arca.fiocruz.br/bitstream/icict/27684/2/Anticoagulant%20activity%20in%20salivary.pdf20f9c27327a2aa8a0475419f48972f21MD52TEXTAnticoagulant activity in salivary.pdf.txtAnticoagulant activity in salivary.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
title Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
spellingShingle Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
Chagas, Andrezza Campos
Moscas negras
Glândulas salivares
Anticoagulante
Oncocercose
Black flies
Salivary glands
Anticoagulant
Onchocerciasis
title_short Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
title_full Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
title_fullStr Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
title_full_unstemmed Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
title_sort Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon
author Chagas, Andrezza Campos
author_facet Chagas, Andrezza Campos
Medeiros, Jansen Fernandes
Astolfi Filho, Spartaco
Py-Daniel, Victor
author_role author
author2 Medeiros, Jansen Fernandes
Astolfi Filho, Spartaco
Py-Daniel, Victor
author2_role author
author
author
dc.contributor.author.fl_str_mv Chagas, Andrezza Campos
Medeiros, Jansen Fernandes
Astolfi Filho, Spartaco
Py-Daniel, Victor
dc.subject.en.pt_BR.fl_str_mv Moscas negras
Glândulas salivares
Anticoagulante
Oncocercose
topic Moscas negras
Glândulas salivares
Anticoagulante
Oncocercose
Black flies
Salivary glands
Anticoagulant
Onchocerciasis
dc.subject.es.pt_BR.fl_str_mv Black flies
Salivary glands
Anticoagulant
Onchocerciasis
description Fundação Oswaldo Cruz. Centro de Pesquisa René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, Brasil.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2018-07-27T12:57:01Z
dc.date.available.fl_str_mv 2018-07-27T12:57:01Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAGAS, Andrezza Campos et al. Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 2, p.174-178, Feb. 2010.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/27684
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762010000200011
identifier_str_mv CHAGAS, Andrezza Campos et al. Anticoagulant activity in salivary gland homogenates of Thyrsopelma guianense (Diptera: Simuliidae), the primary vector of onchocerciasis in the Brazilian Amazon. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 105, n. 2, p.174-178, Feb. 2010.
0074-0276
10.1590/S0074-02762010000200011
url https://www.arca.fiocruz.br/handle/icict/27684
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/27684/1/license.txt
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https://www.arca.fiocruz.br/bitstream/icict/27684/3/Anticoagulant%20activity%20in%20salivary.pdf.txt
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