The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology

Detalhes bibliográficos
Autor(a) principal: Crainey, James L.
Data de Publicação: 2017
Outros Autores: Hurst, Jacob, Lamberton, Poppy H. L., Cheke, Robert A., Griffin, Claire E., Wilson, Michael D., Araújo, Cláudia P. Mendes de, Basáñez, María-Gloria, Post, Rory J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25266
Resumo: We are grateful for the support provided in Ghana by Daniel A. Boakye, Mike Y. Osei-Atweneboana, and Anthony Tetteh-Kumah for the collection of the simuliid larval samples. We are also grateful to the Natural History Museum's DNA sequencing facility, and especially to Julia Llewellyn-Hughes and Lisa Smith, for assisting with the Sanger sequencing used in this study.
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spelling Crainey, James L.Hurst, JacobLamberton, Poppy H. L.Cheke, Robert A.Griffin, Claire E.Wilson, Michael D.Araújo, Cláudia P. Mendes deBasáñez, María-GloriaPost, Rory J.2018-03-12T19:49:07Z2018-03-12T19:49:07Z2017CRAINEY, James Lee et al. The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology. Journal Frontiers in Microbiology, Lausanne, v. 8, p. 1-17, May 2017.https://www.arca.fiocruz.br/handle/icict/252661664-302XWe are grateful for the support provided in Ghana by Daniel A. Boakye, Mike Y. Osei-Atweneboana, and Anthony Tetteh-Kumah for the collection of the simuliid larval samples. We are also grateful to the Natural History Museum's DNA sequencing facility, and especially to Julia Llewellyn-Hughes and Lisa Smith, for assisting with the Sanger sequencing used in this study.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Laboratório de Ecologia de Doenças Transmissíveis na Amazônia. Manaus, AM, Brasil.University of Oxford. Oxford Martin School, Institute for Emerging Infections. Oxford, UK.University of Glasgow. Institute of Biodiversity, Animal Health and Comparative Medicine, Wellcome Centre for Molecular Parasitology. Gasglow, UK.University of Greenwich at Medway. Natural Resources Institute. Chartam, UK.Imperial College London. Department of Infectious Disease Epidemiology, Faculty of Medicine (St Mary's campus), London Centre for Neglected Tropical Disease Research, School of Public Health. London, UK.Natural History Museum. Core Research Laboratories Department, Molecular Biology Laboratories Division. London, UK.University of Ghana. Noguchi Memorial Institute for Medical Research. Accra, Ghana.Liverpool John Moores University. School of Natural Sciences and Psychology. Liverpool, UK.London School of Hygiene and Tropical Medicine. Department of Disease Control, Faculty of Infectious Tropical Diseases. London, UK.Research interest in Wolbachia is growing as new discoveries and technical advancements reveal the public health importance of both naturally occurring and artificial infections. Improved understanding of the Wolbachia bacteriophages (WOs) WOcauB2 and WOcauB3 [belonging to a sub-group of four WOs encoding serine recombinases group 1 (sr1WOs)], has enhanced the prospect of novel tools for the genetic manipulation of Wolbachia. The basic biology of sr1WOs, including host range and mode of genomic integration is, however, still poorly understood. Very few sr1WOs have been described, with two such elements putatively resulting from integrations at the same Wolbachia genome loci, about 2 kb downstream from the FtsZ cell-division gene. Here, we characterize the DNA sequence flanking the FtsZ gene of wDam, a genetically distinct line of Wolbachia isolated from the West African onchocerciasis vector Simulium squamosum E. Using Roche 454 shot-gun and Sanger sequencing, we have resolved >32 kb of WO prophage sequence into three contigs representing three distinct prophage elements. Spanning ≥36 distinct WO open reading frame gene sequences, these prophage elements correspond roughly to three different WO modules: a serine recombinase and replication module (sr1RRM), a head and base-plate module and a tail module. The sr1RRM module contains replication genes and a Holliday junction recombinase and is unique to the sr1 group WOs. In the extreme terminal of the tail module there is a SpvB protein homolog—believed to have insecticidal properties and proposed to have a role in how Wolbachia parasitize their insect hosts. We propose that these wDam prophage modules all derive from a single WO genome, which we have named here sr1WOdamA1. The best-match database sequence for all of our sr1WOdamA1-predicted gene sequences was annotated as of Wolbachia or Wolbachia phage sourced from an arthropod. Clear evidence of exchange between sr1WOdamA1 and other Wolbachia WO phage sequences was also detected. These findings provide insights into how Wolbachia could affect a medically important vector of onchocerciasis, with potential implications for future control methods, as well as supporting the hypothesis that Wolbachia phages do not follow the standard model of phage evolution.engFrontiers Research FoundationWolbachiaFagos de WolbachiaOnchocerciasisOncocercoseWolbachiaWolbachia phagesOnchocerciasisonchocerciasisThe Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83134https://www.arca.fiocruz.br/bitstream/icict/25266/1/license.txt0ab46789d568c4ba27c7b5d29a9fe9c4MD51ORIGINALCrainey_James_etal_ILMD_2017.pdfCrainey_James_etal_ILMD_2017.pdfArticle 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dc.title.pt_BR.fl_str_mv The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
title The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
spellingShingle The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
Crainey, James L.
Wolbachia
Fagos de Wolbachia
Onchocerciasis
Oncocercose
Wolbachia
Wolbachia phages
Onchocerciasis
onchocerciasis
title_short The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
title_full The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
title_fullStr The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
title_full_unstemmed The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
title_sort The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology
author Crainey, James L.
author_facet Crainey, James L.
Hurst, Jacob
Lamberton, Poppy H. L.
Cheke, Robert A.
Griffin, Claire E.
Wilson, Michael D.
Araújo, Cláudia P. Mendes de
Basáñez, María-Gloria
Post, Rory J.
author_role author
author2 Hurst, Jacob
Lamberton, Poppy H. L.
Cheke, Robert A.
Griffin, Claire E.
Wilson, Michael D.
Araújo, Cláudia P. Mendes de
Basáñez, María-Gloria
Post, Rory J.
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Crainey, James L.
Hurst, Jacob
Lamberton, Poppy H. L.
Cheke, Robert A.
Griffin, Claire E.
Wilson, Michael D.
Araújo, Cláudia P. Mendes de
Basáñez, María-Gloria
Post, Rory J.
dc.subject.other.pt_BR.fl_str_mv Wolbachia
Fagos de Wolbachia
Onchocerciasis
Oncocercose
topic Wolbachia
Fagos de Wolbachia
Onchocerciasis
Oncocercose
Wolbachia
Wolbachia phages
Onchocerciasis
onchocerciasis
dc.subject.en.pt_BR.fl_str_mv Wolbachia
Wolbachia phages
Onchocerciasis
onchocerciasis
description We are grateful for the support provided in Ghana by Daniel A. Boakye, Mike Y. Osei-Atweneboana, and Anthony Tetteh-Kumah for the collection of the simuliid larval samples. We are also grateful to the Natural History Museum's DNA sequencing facility, and especially to Julia Llewellyn-Hughes and Lisa Smith, for assisting with the Sanger sequencing used in this study.
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-12T19:49:07Z
dc.date.available.fl_str_mv 2018-03-12T19:49:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CRAINEY, James Lee et al. The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology. Journal Frontiers in Microbiology, Lausanne, v. 8, p. 1-17, May 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25266
dc.identifier.eissn.none.fl_str_mv 1664-302X
identifier_str_mv CRAINEY, James Lee et al. The Genomic Architecture of Novel Simulium damnosum Wolbachia Prophage Sequence Elements and Implications for Onchocerciasis Epidemiology. Journal Frontiers in Microbiology, Lausanne, v. 8, p. 1-17, May 2017.
1664-302X
url https://www.arca.fiocruz.br/handle/icict/25266
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Research Foundation
publisher.none.fl_str_mv Frontiers Research Foundation
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/25266/2/Crainey_James_etal_ILMD_2017.pdf
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