Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2018 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27643 |
Resumo: | 2020-05-01 |
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Silva, J. N.Miranda, L. H. M.Menezes, R. C.Gremião, I. D. F.Oliveira, R. V. C.Vieira, S. M. M.Conceição-Silva, F.Ferreiro, L.Pereira, S. A.2018-07-25T13:02:47Z2018-07-25T13:02:47Z2018SILVA, J. N. et al. Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats. Journal of Comparative Pathology, v. 160, p. 72-78, 2018.0021-9975https://www.arca.fiocruz.br/handle/icict/2764310.1016/j.jcpa.2018.03.002engComparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Catsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-05-01Universidade Federal do Rio Grande do Sul. Faculdade de Veterinária. Agronomia, Porto Alegre, Rio Grande do Sul / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio Grande do Sul. Faculdade de Veterinaria. Agronomia, Porto Alegre, Rio Grande do Sul.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Rio de Janeiro, RJ, Brasil.Sporotrichosis is caused by species of fungi within the Sporothrix schenckii complex that infect man and animals. In Rio de Janeiro, Brazil, an epidemic has been observed since 1998, with most of the cases being related to transmission from infected cats. Although the definitive diagnosis of feline sporotrichosis is made by fungal culture, cytopathological and histopathological examinations are used routinely, because the long culture period may delay treatment onset. However, alternative methods are desirable in cases of low fungal burden. Immunohistochemistry (IHC) has been described as a sensitive method for diagnosing human and canine sporotrichosis, but there are no reports of its application to cats. The aim of this study was to analyse the sensitivity of cytopathological examination (Quick Panoptic method), histopathology (Grocott silver stain) and anti-Sporothrix IHC by blinded comparisons, using fungal culture as the reference standard. Samples were collected from 184 cats with sporotrichosis that exhibited skin ulcers. The sensitivities of Grocott silver stain, cytopathological examination and IHC were 91.3%, 87.0% and 88.6%, respectively. Grocott silver stain showed the best performance. IHC showed high sensitivity, as did cytopathological examination and these may be considered as alternative methodologies. When the three methods were combined, the diagnosis was established in 180 (97.8%) out of 184 cases. Taken together, these findings indicate the need to implement these methods as routine tools for the early diagnosis of sporotrichosis in cats, notably when fungal culture is not available.HistopathologyImmunohistochemistryFeline sporotrichosisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83100https://www.arca.fiocruz.br/bitstream/icict/27643/1/license.txte0c0e718db411cbd1e3f91252b389ec2MD51ORIGINALJessica Silva et al. 2018-s2.0-S0021997517301895-main.pdfJessica Silva et al. 2018-s2.0-S0021997517301895-main.pdfapplication/pdf1753081https://www.arca.fiocruz.br/bitstream/icict/27643/2/Jessica%20Silva%20et%20al.%202018-s2.0-S0021997517301895-main.pdfcb67c7ef1e851e14c3d5ca4a77370f9cMD52TEXTJessica Silva et al. 2018-s2.0-S0021997517301895-main.pdf.txtJessica Silva et al. 2018-s2.0-S0021997517301895-main.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| title |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| spellingShingle |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats Silva, J. N. Histopathology Immunohistochemistry Feline sporotrichosis |
| title_short |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| title_full |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| title_fullStr |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| title_full_unstemmed |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| title_sort |
Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats |
| author |
Silva, J. N. |
| author_facet |
Silva, J. N. Miranda, L. H. M. Menezes, R. C. Gremião, I. D. F. Oliveira, R. V. C. Vieira, S. M. M. Conceição-Silva, F. Ferreiro, L. Pereira, S. A. |
| author_role |
author |
| author2 |
Miranda, L. H. M. Menezes, R. C. Gremião, I. D. F. Oliveira, R. V. C. Vieira, S. M. M. Conceição-Silva, F. Ferreiro, L. Pereira, S. A. |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, J. N. Miranda, L. H. M. Menezes, R. C. Gremião, I. D. F. Oliveira, R. V. C. Vieira, S. M. M. Conceição-Silva, F. Ferreiro, L. Pereira, S. A. |
| dc.subject.en.pt_BR.fl_str_mv |
Histopathology Immunohistochemistry Feline sporotrichosis |
| topic |
Histopathology Immunohistochemistry Feline sporotrichosis |
| description |
2020-05-01 |
| publishDate |
2018 |
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2018-07-25T13:02:47Z |
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2018-07-25T13:02:47Z |
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2018 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, J. N. et al. Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats. Journal of Comparative Pathology, v. 160, p. 72-78, 2018. |
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https://www.arca.fiocruz.br/handle/icict/27643 |
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0021-9975 |
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10.1016/j.jcpa.2018.03.002 |
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SILVA, J. N. et al. Comparison of the Sensitivity of Three Methods for the Early Diagnosis of Sporotrichosis in Cats. Journal of Comparative Pathology, v. 160, p. 72-78, 2018. 0021-9975 10.1016/j.jcpa.2018.03.002 |
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