The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.

Detalhes bibliográficos
Autor(a) principal: Wilson, R. Alan
Data de Publicação: 2015
Outros Autores: Li, Xiao Hong, MacDonald, Sandy, Neves, Leandro Xavier, Souza, Juliana Vitoriano, Leite, Luciana C. C, Farias, Leonardo Paiva, James, Sally, Ashton, Peter D, DeMarco, Ricardo, Borges, William de Castro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/13947
Resumo: University of York. Centre for Immunology and Infection. Department of Biology. Heslington, York, United Kingdom
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spelling Wilson, R. AlanLi, Xiao HongMacDonald, SandyNeves, Leandro XavierSouza, Juliana VitorianoLeite, Luciana C. CFarias, Leonardo PaivaJames, SallyAshton, Peter DDeMarco, RicardoBorges, William de Castro2016-04-20T16:50:30Z2016-04-20T16:50:30Z2015WILSON, R. A. et al. The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing. PLoS Neglected Tropical Diseases, v. 9, n. 12, p. e0004272, 2015.1935-2735https://www.arca.fiocruz.br/handle/icict/1394710.1371/journal.pntd.0004272engPublic Library of ScienceThe Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of York. Centre for Immunology and Infection. Department of Biology. Heslington, York, United KingdomUniversity of York. Centre for Immunology and Infection. Department of Biology. Heslington, York, United Kingdom / National Institute of Parasitic Diseases. Chinese Center for Disease Control and Prevention. Shanghai, People’s Republic of ChinaUniversity of York. Genomics and Bioinformatics Laboratory. Department of Biology. Heslington, York, United KingdomUniversidade Federal de Ouro Preto. Campus Morro do Cruzeiro. Departamento de Ciências Biológicas. Ouro Preto, MG, BrasilInstituto Butantan. Centro Biotecnologia. São Paulo, SP, BrasilInstituto Butantan. Centro Biotecnologia. São Paulo, SP, BrasilInstituto Butantan. Centro Biotecnologia. São Paulo, SP, Brasil / Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of York. Genomics and Bioinformatics Laboratory. Department of Biology. Heslington, York, United KingdomUniversity of York. Genomics and Bioinformatics Laboratory. Department of Biology. Heslington, York, United KingdomUniversidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, BrasilUniversidade Federal de Ouro Preto. Campus Morro do Cruzeiro. Departamento de Ciências Biológicas. Ouro Preto, MG, BrasilBACKGROUND: The schistosome esophagus is divided into anterior and posterior compartments, each surrounded by a dense cluster of gland cell bodies, the source of distinct secretory vesicles discharged into the lumen to initiate the processing of ingested blood. Erythrocytes are lysed in the lumen, leucocytes are tethered and killed and platelets are eliminated. We know little about the proteins secreted from the two glands that mediate these biological processes. METHODOLOGY/PRINCIPAL FINDINGS: We have used subtractive RNA-Seq to characterise the complement of genes that are differentially expressed in a head preparation, compared to matched tissues from worm tails. The expression site of representative highlighted genes was then validated using whole munt in situ hybridisation (WISH). Mapping of transcript reads to the S. mansoni genome assembly using Cufflinks identified ~90 genes that were differentially expressed >fourfold in the head preparation; ~50 novel transcripts were also identified by de novo assembly using Trinity. The largest subset (27) of secreted proteins was encoded by microexon genes (MEGs), the most intense focus identified to date. Expression of three (MEGs 12, 16, 17) was confirmed in the anterior gland and five (MEGs 8.1, 9, 11, 15 and 22) in the posterior gland. The other major subset comprised nine lysosomal hydrolases (aspartyl proteases, phospholipases and palmitoyl thioesterase), again localised to the glands. CONCLUSIONS: A proportion of the MEG-encoded secretory proteins can be classified by their primary structure. We have suggested testable hypotheses about how they might function, in conjunction with the lysosomal hydrolases, to mediate the biological processes that occur in the esophagus lumen. Antibodies bind to the esophageal secretions in both permissive and self-curing hosts, suggesting that the proteins represent a novel panel of untested vaccine candidates. A second major task is to identify which of them can serve as immune targets.Perfilação da Expressão GênicaProteínas de Helminto/biossínteseHidrolases/biossínteseSchistosoma/enzimologiaAnimaisEsôfago/enzimologiaFemininoProteínas de Helminto/genéticaHidrolases/genéticaHibridização In SituMasculinoCamundongos Endogâmicos BALB Cinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/13947/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWilson RA The schistosome ....pdfWilson RA The schistosome ....pdfapplication/pdf7435659https://www.arca.fiocruz.br/bitstream/icict/13947/2/Wilson%20RA%20The%20schistosome%20....pdf5180aa5dfd347e7bae9d10411eff81f9MD52TEXTWilson RA The schistosome ....pdf.txtWilson RA The schistosome ....pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
title The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
spellingShingle The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
Wilson, R. Alan
Perfilação da Expressão Gênica
Proteínas de Helminto/biossíntese
Hidrolases/biossíntese
Schistosoma/enzimologia
Animais
Esôfago/enzimologia
Feminino
Proteínas de Helminto/genética
Hidrolases/genética
Hibridização In Situ
Masculino
Camundongos Endogâmicos BALB C
title_short The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
title_full The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
title_fullStr The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
title_full_unstemmed The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
title_sort The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing.
author Wilson, R. Alan
author_facet Wilson, R. Alan
Li, Xiao Hong
MacDonald, Sandy
Neves, Leandro Xavier
Souza, Juliana Vitoriano
Leite, Luciana C. C
Farias, Leonardo Paiva
James, Sally
Ashton, Peter D
DeMarco, Ricardo
Borges, William de Castro
author_role author
author2 Li, Xiao Hong
MacDonald, Sandy
Neves, Leandro Xavier
Souza, Juliana Vitoriano
Leite, Luciana C. C
Farias, Leonardo Paiva
James, Sally
Ashton, Peter D
DeMarco, Ricardo
Borges, William de Castro
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Wilson, R. Alan
Li, Xiao Hong
MacDonald, Sandy
Neves, Leandro Xavier
Souza, Juliana Vitoriano
Leite, Luciana C. C
Farias, Leonardo Paiva
James, Sally
Ashton, Peter D
DeMarco, Ricardo
Borges, William de Castro
dc.subject.decs.pt_BR.fl_str_mv Perfilação da Expressão Gênica
Proteínas de Helminto/biossíntese
Hidrolases/biossíntese
Schistosoma/enzimologia
Animais
Esôfago/enzimologia
Feminino
Proteínas de Helminto/genética
Hidrolases/genética
Hibridização In Situ
Masculino
Camundongos Endogâmicos BALB C
topic Perfilação da Expressão Gênica
Proteínas de Helminto/biossíntese
Hidrolases/biossíntese
Schistosoma/enzimologia
Animais
Esôfago/enzimologia
Feminino
Proteínas de Helminto/genética
Hidrolases/genética
Hibridização In Situ
Masculino
Camundongos Endogâmicos BALB C
description University of York. Centre for Immunology and Infection. Department of Biology. Heslington, York, United Kingdom
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-04-20T16:50:30Z
dc.date.available.fl_str_mv 2016-04-20T16:50:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv WILSON, R. A. et al. The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing. PLoS Neglected Tropical Diseases, v. 9, n. 12, p. e0004272, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/13947
dc.identifier.issn.none.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0004272
identifier_str_mv WILSON, R. A. et al. The Schistosome Esophagus Is a 'Hotspot' for Microexon and Lysosomal Hydrolase Gene Expression: Implications for Blood Processing. PLoS Neglected Tropical Diseases, v. 9, n. 12, p. e0004272, 2015.
1935-2735
10.1371/journal.pntd.0004272
url https://www.arca.fiocruz.br/handle/icict/13947
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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