pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation

Detalhes bibliográficos
Autor(a) principal: Kugeratski, Fernanda G.
Data de Publicação: 2015
Outros Autores: Batista, Michel, Inoue, Alexandre Haruo, Ramos, Bruno Dias, Krieger, Marco Aurélio, Marchini, Fabrício Klerynton
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15562
Resumo: Financial support: CNPq, IBMP
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spelling Kugeratski, Fernanda G.Batista, MichelInoue, Alexandre HaruoRamos, Bruno DiasKrieger, Marco AurélioMarchini, Fabrício Klerynton2016-08-30T18:57:34Z2016-08-30T18:57:34Z2015KUGERATSKI, Fernanda. G. et al. pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation. Memórias do Instituto Oswaldo Cruz. V.110, n.5, p. 687-90, 2015.0074-0276https://www.arca.fiocruz.br/handle/icict/1556210.1590/0074-02760150074Financial support: CNPq, IBMPFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Genômica Funcional. Curitiba, PR, Brasil.The functional characterisation of thousands of Trypanosoma cruzi genes remains a challenge. Reverse genetics approaches compatible with high-throughput cloning strategies can provide the tool needed to tackle this challenge. We previously published the pTcGW platform, composed by plasmid vectors carrying different options of N-terminal fusion tags based on Gateway® technology. Here, we present an improved 1.1 version of pTcGW vectors, which is characterised by a fully flexible structure allowing an easy customisation of each element of the vectors in a single cloning step. Additionally, both N and C-terminal fusions are available with new tag options for protein complexes purification. Three of the newly created vectors were successfully used to determine the cellular localisation of four T. cruzi proteins. The 1.1 version of pTcGW platform can be used in a variety of assays, such as protein overexpression, identification of protein-protein interaction and protein localisation. This powerful and versatile tool allows adding valuable functional information to T. cruzi genes and is freely available for scientific community.porpTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleTrypanosoma cruziplasmid vectorspTcGW platformreverse geneticscloninggene characterisationVetores GenéticosClonagem de OrganismosExpressão GênicaGenética Reversainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/15562/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALpTcGW plasmid vectors 1.1 version.pdfpTcGW plasmid vectors 1.1 version.pdfapplication/pdf1164502https://www.arca.fiocruz.br/bitstream/icict/15562/2/pTcGW%20plasmid%20vectors%201.1%20version.pdf81a9deb7fad0f58df75e85f0f7a9d2a6MD52TEXTpTcGW plasmid vectors 1.1 version.pdf.txtpTcGW plasmid vectors 1.1 version.pdf.txtExtracted texttext/plain15625https://www.arca.fiocruz.br/bitstream/icict/15562/3/pTcGW%20plasmid%20vectors%201.1%20version.pdf.txt8d2a99a34f7c28431d759c2042959735MD53icict/155622019-10-31 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dc.title.pt_BR.fl_str_mv pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
title pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
spellingShingle pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
Kugeratski, Fernanda G.
Trypanosoma cruzi
plasmid vectors
pTcGW platform
reverse genetics
cloning
gene characterisation
Vetores Genéticos
Clonagem de Organismos
Expressão Gênica
Genética Reversa
title_short pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
title_full pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
title_fullStr pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
title_full_unstemmed pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
title_sort pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation
author Kugeratski, Fernanda G.
author_facet Kugeratski, Fernanda G.
Batista, Michel
Inoue, Alexandre Haruo
Ramos, Bruno Dias
Krieger, Marco Aurélio
Marchini, Fabrício Klerynton
author_role author
author2 Batista, Michel
Inoue, Alexandre Haruo
Ramos, Bruno Dias
Krieger, Marco Aurélio
Marchini, Fabrício Klerynton
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Kugeratski, Fernanda G.
Batista, Michel
Inoue, Alexandre Haruo
Ramos, Bruno Dias
Krieger, Marco Aurélio
Marchini, Fabrício Klerynton
dc.subject.en.pt_BR.fl_str_mv Trypanosoma cruzi
plasmid vectors
pTcGW platform
reverse genetics
cloning
gene characterisation
topic Trypanosoma cruzi
plasmid vectors
pTcGW platform
reverse genetics
cloning
gene characterisation
Vetores Genéticos
Clonagem de Organismos
Expressão Gênica
Genética Reversa
dc.subject.decs.pt_BR.fl_str_mv Vetores Genéticos
Clonagem de Organismos
Expressão Gênica
Genética Reversa
description Financial support: CNPq, IBMP
publishDate 2015
dc.date.issued.fl_str_mv 2015
dc.date.accessioned.fl_str_mv 2016-08-30T18:57:34Z
dc.date.available.fl_str_mv 2016-08-30T18:57:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv KUGERATSKI, Fernanda. G. et al. pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation. Memórias do Instituto Oswaldo Cruz. V.110, n.5, p. 687-90, 2015.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15562
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-02760150074
identifier_str_mv KUGERATSKI, Fernanda. G. et al. pTcGW plasmid vectors 1.1 version: a versatile tool for Trypanosoma cruzi gene characterisation. Memórias do Instituto Oswaldo Cruz. V.110, n.5, p. 687-90, 2015.
0074-0276
10.1590/0074-02760150074
url https://www.arca.fiocruz.br/handle/icict/15562
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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