Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review

Detalhes bibliográficos
Autor(a) principal: Degrave, Wim M.
Data de Publicação: 1994
Outros Autores: Fernandes, Octavio, Campbell, David, Bozza, Marcelo, Lopes, Ulisses
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36819
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Degrave, Wim M.Fernandes, OctavioCampbell, DavidBozza, MarceloLopes, Ulisses2019-11-03T15:00:25Z2019-11-03T15:00:25Z1994DEGRAVE, Wim et al. Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 3, p. 463-469, July/Sept. 1994.0074-0276https://www.arca.fiocruz.br/handle/icict/3681910.1590/S0074-027619940003000321678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.DNA de CinetoplastoLeishmaniaReação em Cadeia da PolimeraseHibridizaçãoSondas MolecularesDNA de CinetoplastoLeishmaniaPolymerase chain reactionHybridizationMolecular probesMini-exon geneMinicirclekDNAUse of molecular probes and PCR for detection and typing of Leishmania - a mini-reviewinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Departamento de Patologia. Rio de Janeiro, RJ, Brasil.University of California. School of Medicine. Department of Microbiology and Immunology. Los Angeles, USA.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Parasitologia Molecular. Rio de Janeiro, RJ, Brasil.The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36819/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWinDegrave_MarceloBozza_etal_IOC_1994.pdfWinDegrave_MarceloBozza_etal_IOC_1994.pdfapplication/pdf486987https://www.arca.fiocruz.br/bitstream/icict/36819/2/WinDegrave_MarceloBozza_etal_IOC_1994.pdf17c053d834378c70c31bc2437fa25be2MD52TEXTWinDegrave_MarceloBozza_etal_IOC_1994.pdf.txtWinDegrave_MarceloBozza_etal_IOC_1994.pdf.txtExtracted texttext/plain7https://www.arca.fiocruz.br/bitstream/icict/36819/3/WinDegrave_MarceloBozza_etal_IOC_1994.pdf.txt212b0306580d4f0044d18f9a3edcc832MD53icict/368192019-12-13 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dc.title.pt_BR.fl_str_mv Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
spellingShingle Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
Degrave, Wim M.
DNA de Cinetoplasto
Leishmania
Reação em Cadeia da Polimerase
Hibridização
Sondas Moleculares
DNA de Cinetoplasto
Leishmania
Polymerase chain reaction
Hybridization
Molecular probes
Mini-exon gene
Minicircle
kDNA
title_short Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_full Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_fullStr Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_full_unstemmed Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_sort Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
author Degrave, Wim M.
author_facet Degrave, Wim M.
Fernandes, Octavio
Campbell, David
Bozza, Marcelo
Lopes, Ulisses
author_role author
author2 Fernandes, Octavio
Campbell, David
Bozza, Marcelo
Lopes, Ulisses
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Degrave, Wim M.
Fernandes, Octavio
Campbell, David
Bozza, Marcelo
Lopes, Ulisses
dc.subject.mesh.pt_BR.fl_str_mv DNA de Cinetoplasto
topic DNA de Cinetoplasto
Leishmania
Reação em Cadeia da Polimerase
Hibridização
Sondas Moleculares
DNA de Cinetoplasto
Leishmania
Polymerase chain reaction
Hybridization
Molecular probes
Mini-exon gene
Minicircle
kDNA
dc.subject.other.pt_BR.fl_str_mv Leishmania
Reação em Cadeia da Polimerase
Hibridização
Sondas Moleculares
DNA de Cinetoplasto
dc.subject.en.pt_BR.fl_str_mv Leishmania
Polymerase chain reaction
Hybridization
Molecular probes
Mini-exon gene
Minicircle
kDNA
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1994
dc.date.issued.fl_str_mv 1994
dc.date.accessioned.fl_str_mv 2019-11-03T15:00:25Z
dc.date.available.fl_str_mv 2019-11-03T15:00:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv DEGRAVE, Wim et al. Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 3, p. 463-469, July/Sept. 1994.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36819
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761994000300032
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv DEGRAVE, Wim et al. Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 89, n. 3, p. 463-469, July/Sept. 1994.
0074-0276
10.1590/S0074-02761994000300032
1678-8060
url https://www.arca.fiocruz.br/handle/icict/36819
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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