Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review

Detalhes bibliográficos
Autor(a) principal: Degrave,Win
Data de Publicação: 1994
Outros Autores: Fernandes,Octavio, Campbell,David, Bozza,Marcelo, Lopes,Ulisses
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032
Resumo: The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
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spelling Use of molecular probes and PCR for detection and typing of Leishmania - a mini-reviewLeishmaniapolymerase chain reactionhybridizationmolecular probesmini-exon geneminicirclekDNAThe use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.Instituto Oswaldo Cruz, Ministério da Saúde1994-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032Memórias do Instituto Oswaldo Cruz v.89 n.3 1994reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761994000300032info:eu-repo/semantics/openAccessDegrave,WinFernandes,OctavioCampbell,DavidBozza,MarceloLopes,Ulisseseng2020-04-25T17:47:18Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:06:10.322Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
spellingShingle Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
Degrave,Win
Leishmania
polymerase chain reaction
hybridization
molecular probes
mini-exon gene
minicircle
kDNA
title_short Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_full Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_fullStr Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_full_unstemmed Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
title_sort Use of molecular probes and PCR for detection and typing of Leishmania - a mini-review
author Degrave,Win
author_facet Degrave,Win
Fernandes,Octavio
Campbell,David
Bozza,Marcelo
Lopes,Ulisses
author_role author
author2 Fernandes,Octavio
Campbell,David
Bozza,Marcelo
Lopes,Ulisses
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Degrave,Win
Fernandes,Octavio
Campbell,David
Bozza,Marcelo
Lopes,Ulisses
dc.subject.por.fl_str_mv Leishmania
polymerase chain reaction
hybridization
molecular probes
mini-exon gene
minicircle
kDNA
topic Leishmania
polymerase chain reaction
hybridization
molecular probes
mini-exon gene
minicircle
kDNA
dc.description.none.fl_txt_mv The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
description The use of molecular tools to detect and type Leishmania species in humans, reservoirs or sandflies has been pursued using different approaches. The polymerase chain reaction provided sensitivity to case this task, since the use of hybridization procedures alone employing specifics probes is hampered due to the low detection limit. In this report, we describe the different molecular targets used in our laboratory, aiming at the detection and specific typing of these protozoa. Different kits based on hybridization assays and PCR amplification using kinetoplast and nuclear targets are described and the results obtained from their use are reported.
publishDate 1994
dc.date.none.fl_str_mv 1994-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761994000300032
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761994000300032
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.89 n.3 1994
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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