Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)

Detalhes bibliográficos
Autor(a) principal: Souza, Ligia Cristina Kalb
Data de Publicação: 2013
Outros Autores: Pinho, Rosana Elisa Gonçalves, Lima, Carla Vanessa de Paula, Fragoso, Stenio Perdigão, Soares, Maurilio José
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/15599
Resumo: CNPq, Fiocruz
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spelling Souza, Ligia Cristina KalbPinho, Rosana Elisa GonçalvesLima, Carla Vanessa de PaulaFragoso, Stenio PerdigãoSoares, Maurilio José2016-09-02T17:22:39Z2016-09-02T17:22:39Z2013SOUZA, Ligia Cristina Kalb et al. Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea). Mem. Inst. Oswaldo Cruz, v. 108, n. 5, p. 631-636, 2013..0074-0276https://www.arca.fiocruz.br/handle/icict/15599CNPq, FiocruzFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Heteroxenic and monoxenic trypanosomatids were screened for the presence of actin using a mouse polyclonal antibody produced against the entire sequence of the Trypanosoma cruzi actin gene, encoding a 41.9 kDa protein. Western blot analysis showed that this antibody reacted with a polypeptide of approximately 42 kDa in the whole-cell lysates of parasites targeting mammals (T. cruzi, Trypanosoma brucei and Leishmania major), insects (Angomonas deanei, Crithidia fasciculata, Herpetomonas samuelpessoai and Strigomonas culicis) and plants (Phytomonas serpens). A single polypeptide of approximately 42 kDa was detected in the whole-cell lysates of T. cruzi cultured epimastigotes, metacyclic trypomastigotes and amastigotes at similar protein expression levels. Confocal microscopy showed that actin was expressed throughout the cytoplasm of all the tested trypanosomatids. These data demonstrate that actin expression is widespread in trypanosomatids.porActin expression in trypanosomatids (Euglenozoa: Kinetoplastea)info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleactinconfocal microscopytrypanosomatidsTrypanosoma cruziMicroscopia ConfocalTrypanosomatinaActinasinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/15599/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALActin expression in trypanosomatids.pdfActin expression in trypanosomatids.pdfapplication/pdf804096https://www.arca.fiocruz.br/bitstream/icict/15599/2/Actin%20expression%20in%20trypanosomatids.pdf9119ee0e5ba51b45a7435c240f5831daMD52TEXTActin expression in trypanosomatids.pdf.txtActin expression in trypanosomatids.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
spellingShingle Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
Souza, Ligia Cristina Kalb
actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
Microscopia Confocal
Trypanosomatina
Actinas
title_short Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_full Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_fullStr Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_full_unstemmed Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
title_sort Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea)
author Souza, Ligia Cristina Kalb
author_facet Souza, Ligia Cristina Kalb
Pinho, Rosana Elisa Gonçalves
Lima, Carla Vanessa de Paula
Fragoso, Stenio Perdigão
Soares, Maurilio José
author_role author
author2 Pinho, Rosana Elisa Gonçalves
Lima, Carla Vanessa de Paula
Fragoso, Stenio Perdigão
Soares, Maurilio José
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Souza, Ligia Cristina Kalb
Pinho, Rosana Elisa Gonçalves
Lima, Carla Vanessa de Paula
Fragoso, Stenio Perdigão
Soares, Maurilio José
dc.subject.en.pt_BR.fl_str_mv actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
topic actin
confocal microscopy
trypanosomatids
Trypanosoma cruzi
Microscopia Confocal
Trypanosomatina
Actinas
dc.subject.decs.pt_BR.fl_str_mv Microscopia Confocal
Trypanosomatina
Actinas
description CNPq, Fiocruz
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2016-09-02T17:22:39Z
dc.date.available.fl_str_mv 2016-09-02T17:22:39Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Ligia Cristina Kalb et al. Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea). Mem. Inst. Oswaldo Cruz, v. 108, n. 5, p. 631-636, 2013..
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/15599
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
identifier_str_mv SOUZA, Ligia Cristina Kalb et al. Actin expression in trypanosomatids (Euglenozoa: Kinetoplastea). Mem. Inst. Oswaldo Cruz, v. 108, n. 5, p. 631-636, 2013..
0074-0276
url https://www.arca.fiocruz.br/handle/icict/15599
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/15599/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/15599/2/Actin%20expression%20in%20trypanosomatids.pdf
https://www.arca.fiocruz.br/bitstream/icict/15599/3/Actin%20expression%20in%20trypanosomatids.pdf.txt
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