Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/30426 |
Resumo: | 2020-01-01 |
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Ker, Henrique GamaSoares, Rodrigo Dian de Oliveira AguiarRoatt, Bruno MendesMoreira, Nádia das DoresVital, Wendel CouraCarneiro, Cláudia MartinsCarvalho, Andréa Teixeira deMartins Filho, Olindo AssisGiunchetti, Rodolfo CordeiroLemos, Denise da SilveiraReis, Alexandre Barbosa2018-12-07T17:51:48Z2018-12-07T17:51:48Z2013KER, Henrique Gama et al. Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis. Diagn Microbiol Infect Dis., v. 76, n. 4, p. 470-476, 20130732-8893https://www.arca.fiocruz.br/handle/icict/3042610.1016/j.diagmicrobio.2013.04.007engElsevier Biomedicalcitometria de fluxoFC-AFPA-IgGIFATELISALeishmaniose visceral caninadiagnosticoFlow cytometryFC-AFPA-IgGIFATELISACanine visceral leishmaniasisDiagnosisEffect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Renè Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Renè Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Biologia das Interações Celulares. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Laboratório de Imunologia e Genômica de Parasitos. Belo Horizonte, MG, BrazilUniversidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Laboratório de Pesquisas Clínicas. Ouro Preto, MG, Brazil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrazilThe control of canine visceral leishmaniasis (CVL) is imperative, but euthanasia of seropositive dogs has been highly criticized. Commonly used, immunodiagnostic tests, including Dual-Path Platform®, enzyme-linked immunosorbent assay, and immunofluorescent antibody test, have failed at detecting asymptomatic dogs in endemic areas. In this context, new serological methods are needed. Flow cytometry serology has demonstrated potential as a test with excellent performance for CVL. In this study, we proposed to establish the best conditions for preserving Leishmania infantum promastigote antigens employed in this serology test. During 12 months of follow-up, promastigotes were maintained in different preservatives (phosphate-buffered saline with 3% fetal bovine serum, phenol 0.35%, thimerosal 0.01%, and formaldehyde 0.5%) and stored at 3 distinct temperatures (25 °C, 4 °C, and −20 °C). During the study period, the morphological characteristics of the promastigotes were assessed by flow cytometry according to the forward and side scatter parameters and also under optical microscopic analysis. Reactivity performance was evaluated as the percentage of positive fluorescent parasites in the sera of naturally infected and noninfected dogs. Microbiological analysis was performed at 2 time points, the first and sixth months, to rule out contamination of stored promastigotes. Taken together, our results indicated that the best conditions to preserve fixed L. infantum antigens were storage in formaldehyde at 4 °C. Promastigotes presented the best morphological profile, with appropriate antigenic stability even at 4 °C, in an inexpensive preservative for a long period of conservation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/30426/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALEffect of the preservative and temperature conditions.pdfEffect of the preservative and temperature conditions.pdfapplication/pdf6569260https://www.arca.fiocruz.br/bitstream/icict/30426/2/Effect%20of%20the%20preservative%20and%20temperature%20conditions.pdf6a5a0e1092da17607b8d5a760f79e29eMD52TEXTEffect of the preservative and temperature conditions.pdf.txtEffect of the preservative and temperature conditions.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| title |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| spellingShingle |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis Ker, Henrique Gama citometria de fluxo FC-AFPA-IgG IFAT ELISA Leishmaniose visceral canina diagnostico Flow cytometry FC-AFPA-IgG IFAT ELISA Canine visceral leishmaniasis Diagnosis |
| title_short |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| title_full |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| title_fullStr |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| title_full_unstemmed |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| title_sort |
Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis |
| author |
Ker, Henrique Gama |
| author_facet |
Ker, Henrique Gama Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Vital, Wendel Coura Carneiro, Cláudia Martins Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| author_role |
author |
| author2 |
Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Vital, Wendel Coura Carneiro, Cláudia Martins Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ker, Henrique Gama Soares, Rodrigo Dian de Oliveira Aguiar Roatt, Bruno Mendes Moreira, Nádia das Dores Vital, Wendel Coura Carneiro, Cláudia Martins Carvalho, Andréa Teixeira de Martins Filho, Olindo Assis Giunchetti, Rodolfo Cordeiro Lemos, Denise da Silveira Reis, Alexandre Barbosa |
| dc.subject.other.pt_BR.fl_str_mv |
citometria de fluxo FC-AFPA-IgG IFAT ELISA Leishmaniose visceral canina diagnostico |
| topic |
citometria de fluxo FC-AFPA-IgG IFAT ELISA Leishmaniose visceral canina diagnostico Flow cytometry FC-AFPA-IgG IFAT ELISA Canine visceral leishmaniasis Diagnosis |
| dc.subject.en.pt_BR.fl_str_mv |
Flow cytometry FC-AFPA-IgG IFAT ELISA Canine visceral leishmaniasis Diagnosis |
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2020-01-01 |
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2013 |
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2013 |
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2018-12-07T17:51:48Z |
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2018-12-07T17:51:48Z |
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KER, Henrique Gama et al. Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis. Diagn Microbiol Infect Dis., v. 76, n. 4, p. 470-476, 2013 |
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https://www.arca.fiocruz.br/handle/icict/30426 |
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0732-8893 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.diagmicrobio.2013.04.007 |
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KER, Henrique Gama et al. Effect of the preservative and temperature conditions on the stability of Leishmania infantum promastigotes antigens applied in a flow cytometry diagnostic method for canine visceral leishmaniasis. Diagn Microbiol Infect Dis., v. 76, n. 4, p. 470-476, 2013 0732-8893 10.1016/j.diagmicrobio.2013.04.007 |
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Elsevier Biomedical |
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Elsevier Biomedical |
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