Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Pereira, Valéria Rêgo Alves
Data de Publicação: 2012
Outros Autores: Reis, Luiza de Campos, Souza, Marina de Assis, Oliveira, Andresa Pereira de, Brito, Maria Edileuza Felinto de, Lage, Paula Souza, Andrade, Mariléia Chaves de, Rocha, Roberta Dias Rodrigues, Martins Filho, Olindo Assis
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7826
Resumo: State of Pernambuco Research Foundation (FACEPE), by the Brazilian National Research Council (CNPq), and by the Oswaldo Cruz Foundation (FIOCRUZ).
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spelling Pereira, Valéria Rêgo AlvesReis, Luiza de CamposSouza, Marina de AssisOliveira, Andresa Pereira deBrito, Maria Edileuza Felinto deLage, Paula SouzaAndrade, Mariléia Chaves deRocha, Roberta Dias RodriguesMartins Filho, Olindo Assis2014-06-17T14:43:21Z2014-06-17T14:43:21Z2012PEREIRA, Valéria Rêgo Alves et al. Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis. Diagnostic Microbiology and Infectious Disease. 2012, vol.74, pp. 292-298https://www.arca.fiocruz.br/handle/icict/782610.1016/j.diagmicrobio.2012.06.025State of Pernambuco Research Foundation (FACEPE), by the Brazilian National Research Council (CNPq), and by the Oswaldo Cruz Foundation (FIOCRUZ).Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Recife, PE, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Belo Horizonte, MG, Brazil/Universidade Estadual de Montes Claros. Montes Claros, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou.Belo Horizonte, MG, Brazil/Centro Universitário Newton Paiva. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou.Belo Horizonte, MG, BrazilThis study aims to investigate a flow cytometry performance–based methodology to detect anti-live (FC-ALPA-IgG) and anti-fixed (FC-AFPA-IgG) Leishmania (Viannia) braziliensis promastigote IgG as a means to monitor post-therapeutic cure of patients with localized cutaneous leishmaniasis (LCL). Serum samples from 30 LCL patients infected with L. (V.) braziliensis were assayed, comparing the IgG reactivity before and after specific treatment with pentavalent antimonial. Reactivities were reported as the percentage of positive fluorescent parasites (PPFP), using a PPFP of 60% as a cut-off value. In the serum dilution of 1:1024, the positive percentage of LCL serum sample for FC-ALPA-IgG and FC-AFPA-IgG was 86% and 90%, respectively, before treatment. Analysis of ∆PPFP that represents the difference between PPFP after and before treatment appeared as a new approach to monitor post-therapeutic IgG reactivity in LCL. Our data support the perspective of using FC-ALPA and FC-AFPA as a useful serologic tool for diagnosis and for post-therapeutic follow-up of LCL patients.engElsevier B.V.Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleLocalized cutaneous leishmaniasisFlow cytometryFC-ALPAFC-AFPAIgGL. (V.) braziliensisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdfEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdfapplication/pdf1302398https://www.arca.fiocruz.br/bitstream/icict/7826/1/Evaluation%20of%20anti-lived%20and%20anti-fixed-%20Leishmania%20%28Viannia%29%20braziliensis%20promastigotes%20IgG%20.pdf22a497d8d77df6cf087a9b6f0a0c93a6MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7826/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdf.txtEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdf.txtExtracted texttext/plain7https://www.arca.fiocruz.br/bitstream/icict/7826/5/Evaluation%20of%20anti-lived%20and%20anti-fixed-%20Leishmania%20%28Viannia%29%20braziliensis%20promastigotes%20IgG%20.pdf.txt212b0306580d4f0044d18f9a3edcc832MD55THUMBNAILEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdf.jpgEvaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG .pdf.jpgGenerated Thumbnailimage/jpeg1940https://www.arca.fiocruz.br/bitstream/icict/7826/4/Evaluation%20of%20anti-lived%20and%20anti-fixed-%20Leishmania%20%28Viannia%29%20braziliensis%20promastigotes%20IgG%20.pdf.jpgf0667d448de29266359b19b7120403caMD54icict/78262022-08-17 09:07:53.423oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-17T12:07:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
title Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
spellingShingle Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
Pereira, Valéria Rêgo Alves
Localized cutaneous leishmaniasis
Flow cytometry
FC-ALPA
FC-AFPA
IgG
L. (V.) braziliensis
title_short Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
title_full Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
title_fullStr Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
title_full_unstemmed Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
title_sort Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis
author Pereira, Valéria Rêgo Alves
author_facet Pereira, Valéria Rêgo Alves
Reis, Luiza de Campos
Souza, Marina de Assis
Oliveira, Andresa Pereira de
Brito, Maria Edileuza Felinto de
Lage, Paula Souza
Andrade, Mariléia Chaves de
Rocha, Roberta Dias Rodrigues
Martins Filho, Olindo Assis
author_role author
author2 Reis, Luiza de Campos
Souza, Marina de Assis
Oliveira, Andresa Pereira de
Brito, Maria Edileuza Felinto de
Lage, Paula Souza
Andrade, Mariléia Chaves de
Rocha, Roberta Dias Rodrigues
Martins Filho, Olindo Assis
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pereira, Valéria Rêgo Alves
Reis, Luiza de Campos
Souza, Marina de Assis
Oliveira, Andresa Pereira de
Brito, Maria Edileuza Felinto de
Lage, Paula Souza
Andrade, Mariléia Chaves de
Rocha, Roberta Dias Rodrigues
Martins Filho, Olindo Assis
dc.subject.en.pt_BR.fl_str_mv Localized cutaneous leishmaniasis
Flow cytometry
FC-ALPA
FC-AFPA
IgG
L. (V.) braziliensis
topic Localized cutaneous leishmaniasis
Flow cytometry
FC-ALPA
FC-AFPA
IgG
L. (V.) braziliensis
description State of Pernambuco Research Foundation (FACEPE), by the Brazilian National Research Council (CNPq), and by the Oswaldo Cruz Foundation (FIOCRUZ).
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-06-17T14:43:21Z
dc.date.available.fl_str_mv 2014-06-17T14:43:21Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv PEREIRA, Valéria Rêgo Alves et al. Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis. Diagnostic Microbiology and Infectious Disease. 2012, vol.74, pp. 292-298
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7826
dc.identifier.doi.none.fl_str_mv 10.1016/j.diagmicrobio.2012.06.025
identifier_str_mv PEREIRA, Valéria Rêgo Alves et al. Evaluation of anti-lived and anti-fixed- Leishmania (Viannia) braziliensis promastigotes IgG antibodies detected by flow cytometry for diagnosis and post-therapeutic cure assessment in localized cutaneous leishmaniasis. Diagnostic Microbiology and Infectious Disease. 2012, vol.74, pp. 292-298
10.1016/j.diagmicrobio.2012.06.025
url https://www.arca.fiocruz.br/handle/icict/7826
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
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