Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses

Detalhes bibliográficos
Autor(a) principal: Naveca, Felipe Gomes
Data de Publicação: 2017
Outros Autores: Nascimento, Valdinete Alves do, Souza, Victor Costa de, Nunes, Bruno Tardelli Diniz, Rodrigues, Daniela Sueli Guerreiro, Vasconcelos, Pedro Fernando da Costa
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/19371
Resumo: Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
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spelling Naveca, Felipe GomesNascimento, Valdinete Alves doSouza, Victor Costa deNunes, Bruno Tardelli DinizRodrigues, Daniela Sueli GuerreiroVasconcelos, Pedro Fernando da Costa2017-06-13T14:42:56Z2017-06-13T14:42:56Z2017NAVECA, Felipe Gomes; et al. Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 112, n. 7, p. 510-513, July 2017.0074-0276https://www.arca.fiocruz.br/handle/icict/1937110.1590/0074-027601600621678-8060porInstituto Oswaldo Cruz, Ministério da SaúdeMultiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like virusesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Instituto Evandro Chagas. Ananindeua, PA, Brasil / Unversidade do Estado do Pará. Belém, PA, Brasil.We describe a sensitive method for simultaneous detection of Oropouche and Oropouche-like viruses carrying the Oropouche S segment, as well as the Mayaro virus, using a multiplexed one-step reverse transcription real-time polymerase chain reaction (RT-qPCR). A chimeric plasmid containing both Mayaro and Oropouche targets was designed and evaluated for the in vitro production of transcribed RNA, which could be easily used as a non-infectious external control. To track false-negative results due to PCR inhibition or equipment malfunction, the MS2 bacteriophage was also included in the multiplex assay as an internal positive control. The specificity of the multiplex assay was evaluated by Primer-Blast analysis against the entire GenBank database, and further against a panel of 17 RNA arboviruses. The results indicated an accurate and highly sensitive assay with amplification efficiency greater than 98% for both targets, and a limit of detection between two and 20 copies per reaction. We believe that the assay described here will provide a tool for Mayaro and Oropouche virus detection, especially in areas where differential diagnosis of Dengue, Zika and Chikungunya viruses should be performed.Real-time PCRMayaro VirusOropouche VirusAmazoninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83133https://www.arca.fiocruz.br/bitstream/icict/19371/1/license.txtf74758116b4c371a58ab8be5ab029452MD51ORIGINALve_Felipe_Gomes_Naveca_etal_ILMD_2017.pdfve_Felipe_Gomes_Naveca_etal_ILMD_2017.pdfapplication/pdf611276https://www.arca.fiocruz.br/bitstream/icict/19371/2/ve_Felipe_Gomes_Naveca_etal_ILMD_2017.pdf7e501740aec08f9832c2d20d41b9f4a9MD52TEXTve_Felipe_Gomes_Naveca_etal_ILMD_2017.pdf.txtve_Felipe_Gomes_Naveca_etal_ILMD_2017.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
title Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
spellingShingle Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
Naveca, Felipe Gomes
Real-time PCR
Mayaro Virus
Oropouche Virus
Amazon
title_short Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
title_full Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
title_fullStr Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
title_full_unstemmed Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
title_sort Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses
author Naveca, Felipe Gomes
author_facet Naveca, Felipe Gomes
Nascimento, Valdinete Alves do
Souza, Victor Costa de
Nunes, Bruno Tardelli Diniz
Rodrigues, Daniela Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
author_role author
author2 Nascimento, Valdinete Alves do
Souza, Victor Costa de
Nunes, Bruno Tardelli Diniz
Rodrigues, Daniela Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Naveca, Felipe Gomes
Nascimento, Valdinete Alves do
Souza, Victor Costa de
Nunes, Bruno Tardelli Diniz
Rodrigues, Daniela Sueli Guerreiro
Vasconcelos, Pedro Fernando da Costa
dc.subject.en.pt_BR.fl_str_mv Real-time PCR
Mayaro Virus
Oropouche Virus
Amazon
topic Real-time PCR
Mayaro Virus
Oropouche Virus
Amazon
description Fundação Oswaldo Cruz. Instituto Leônidas e Maria Deane. Manaus, AM, Brasil.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-06-13T14:42:56Z
dc.date.available.fl_str_mv 2017-06-13T14:42:56Z
dc.date.issued.fl_str_mv 2017
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv NAVECA, Felipe Gomes; et al. Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 112, n. 7, p. 510-513, July 2017.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/19371
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760160062
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv NAVECA, Felipe Gomes; et al. Multiplexed reverse transcription real-time polymerase chain reaction for simultaneous detection of Mayaro, Oropouche, and Oropouche-like viruses. Mem. Inst. Oswaldo Cruz, Rio de Janeiro , v. 112, n. 7, p. 510-513, July 2017.
0074-0276
10.1590/0074-02760160062
1678-8060
url https://www.arca.fiocruz.br/handle/icict/19371
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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