Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2016 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/23745 |
Resumo: | Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. |
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Nogueira, Caio Cesar RichterPaixão, Izabel Christina Nunes de PalmerSantos, Claudio Cesar CirneStephens, Paulo Roberto SoaresVillaça, Roberto CamposPereira, Helena de SouzaTeixeira, Valéria Laneuville2017-12-21T16:04:33Z2017-12-21T16:04:33Z2016NOGUEIRA, Caio Cesar Richter; et al. Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera. Journal of Medicinal Plants Research, v.10, n.35, p.621-625, Sept. 2016.1996-0875https://www.arca.fiocruz.br/handle/icict/2374510.5897/JMPR2016.6208engAcademic JournalsHIV-1Acanthophora spiciferaAlga Marinha vermelhaAtividade antiviralMarine natural productsred seaweedAcanthophora spiciferaHIV-1Antiviral activityAnti-HIV-1 RTAnti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spiciferainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ. Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil.Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil.First generation drugs such as zidovudine have been extensively used in clinical practice, resulting in the development of HIV resistance to these nucleoside analogs. Several studies have demonstrated the effective anti-HIV activity of natural products derived from seaweeds, suggesting promising sources of substances for the development of novel antiviral drugs. In this paper, the antiviral effect of extracts from the red seaweed Acanthophora spicifera on HIV-1 replication was evaluated in vitro. Peripheral blood mononuclear cells obtained using the Ficoll-Hypaque gradient were used for cytotoxicity and antiviral activity testing. The dichloromethane extracts, ethyl acetate, acetone, and methanol were found to have CC50 values of 31±7.4, 45±11, 38±3.5, and 179±25 μg/mL, respectively. With the control, the extract prepared in ethyl acetate inhibited approximately 60% of the viral load, which is the best result among the extracts. This same extract showed an IC50 value of 33.17±4.84 μg/mL for the reverse transcriptase. The EtOAc extract from A. spicifera showed to be an efficient HIV antiviral due to its phenolic compounds, as evaluated by nuclear magnetic resonance.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/23745/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALclaudiocesar_santos_etal_IOC_2016.pdfclaudiocesar_santos_etal_IOC_2016.pdfapplication/pdf510025https://www.arca.fiocruz.br/bitstream/icict/23745/2/claudiocesar_santos_etal_IOC_2016.pdfad479082bf4c7e7c22f0398b90231411MD52TEXTclaudiocesar_santos_etal_IOC_2016.pdf.txtclaudiocesar_santos_etal_IOC_2016.pdf.txtExtracted texttext/plain23136https://www.arca.fiocruz.br/bitstream/icict/23745/3/claudiocesar_santos_etal_IOC_2016.pdf.txt19a34ad75d1084d21bbb0c2f4a572ff8MD53icict/237452023-09-05 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| dc.title.pt_BR.fl_str_mv |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| title |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| spellingShingle |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera Nogueira, Caio Cesar Richter HIV-1 Acanthophora spicifera Alga Marinha vermelha Atividade antiviral Marine natural products red seaweed Acanthophora spicifera HIV-1 Antiviral activity Anti-HIV-1 RT |
| title_short |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| title_full |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| title_fullStr |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| title_full_unstemmed |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| title_sort |
Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera |
| author |
Nogueira, Caio Cesar Richter |
| author_facet |
Nogueira, Caio Cesar Richter Paixão, Izabel Christina Nunes de Palmer Santos, Claudio Cesar Cirne Stephens, Paulo Roberto Soares Villaça, Roberto Campos Pereira, Helena de Souza Teixeira, Valéria Laneuville |
| author_role |
author |
| author2 |
Paixão, Izabel Christina Nunes de Palmer Santos, Claudio Cesar Cirne Stephens, Paulo Roberto Soares Villaça, Roberto Campos Pereira, Helena de Souza Teixeira, Valéria Laneuville |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Caio Cesar Richter Paixão, Izabel Christina Nunes de Palmer Santos, Claudio Cesar Cirne Stephens, Paulo Roberto Soares Villaça, Roberto Campos Pereira, Helena de Souza Teixeira, Valéria Laneuville |
| dc.subject.other.pt_BR.fl_str_mv |
HIV-1 Acanthophora spicifera Alga Marinha vermelha Atividade antiviral |
| topic |
HIV-1 Acanthophora spicifera Alga Marinha vermelha Atividade antiviral Marine natural products red seaweed Acanthophora spicifera HIV-1 Antiviral activity Anti-HIV-1 RT |
| dc.subject.en.pt_BR.fl_str_mv |
Marine natural products red seaweed Acanthophora spicifera HIV-1 Antiviral activity Anti-HIV-1 RT |
| description |
Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Marinha. Niterói, RJ, Brasil / Universidade Federal Fluminense. Instituto de Biologia. Departamento de Biologia Celular e Molecular. Niterói, RJ, Brasil. |
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2016 |
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2016 |
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2017-12-21T16:04:33Z |
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2017-12-21T16:04:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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NOGUEIRA, Caio Cesar Richter; et al. Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera. Journal of Medicinal Plants Research, v.10, n.35, p.621-625, Sept. 2016. |
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https://www.arca.fiocruz.br/handle/icict/23745 |
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1996-0875 |
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10.5897/JMPR2016.6208 |
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NOGUEIRA, Caio Cesar Richter; et al. Anti-HIV-1 activity in human primary cells and Anti-HIV- 1 RT inhibitory activity of extracts from the red seaweed Acanthophora spicifera. Journal of Medicinal Plants Research, v.10, n.35, p.621-625, Sept. 2016. 1996-0875 10.5897/JMPR2016.6208 |
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