Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/8692 |
Resumo: | Universidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, Brazil |
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Gaio, Analiz de OliveiraRodrigues, Rivea Cristina CustodioNascimento, Cássio doSecundino, Nágila Francinete CostaLemos, Francisco Jose AlvesPimenta, Paulo Filemon PaolucciMonesi, Nadia2014-10-29T17:31:45Z2014-10-29T17:31:45Z2011GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011.1756-3305https://www.arca.fiocruz.br/handle/icict/869210.1186/1756-3305-4-237engBioMed CentralUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilFaculdade de Odontologia de Ribeirão Preto. Departamento de Materiais Dentários e Prótese. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilFaculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, BrazilBackground: Bacteria associated with insects can have a substantial impact on the biology and life cycle of their host. The checkerboard DNA-DNA hybridization technique is a semi-quantitative technique that has been previously employed in odontology to detect and quantify a variety of bacterial species in dental samples. Here we tested the applicability of the checkerboard DNA-DNA hybridization technique to detect the presence of Aedes aegypti-associated bacterial species in larvae, pupae and adults of A. aegypti. Findings: Using the checkerboard DNA-DNA hybridization technique we could detect and estimate the number of four bacterial species in total DNA samples extracted from A. aegypti single whole individuals and midguts. A. aegypti associated bacterial species were also detected in the midgut of four other insect species, Lutzomyia longipalpis, Drosophila melanogaster, Bradysia hygida andApis mellifera. Conclusions: Our results demonstrate that the checkerboard DNA-DNA hybridization technique can be employed to study the microbiota composition of mosquitoes. The method has the sensitivity to detect bacteria in single individuals, as well as in a single organ, and therefore can be employed to evaluate the differences in bacterial counts amongst individuals in a given mosquito population. We suggest that the checkerboard DNA-DNA hybridization technique is a straightforward technique that can be widely used for the characterization of the microbiota in mosquito populations.CheckerboardDNA hybridizationAedes aegyptibacteriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8692/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdfUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdfapplication/pdf1405994https://www.arca.fiocruz.br/bitstream/icict/8692/2/Use%20of%20the%20checkerboard%20DNA-DNA%20hybridization%20technique%20for%20bacteria%20detection%20in%20Aedes%20aegypti.pdf8dfe54019468f8ccdcbcc2544c8c5b14MD52TEXTUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdf.txtUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdf.txtExtracted texttext/plain19602https://www.arca.fiocruz.br/bitstream/icict/8692/3/Use%20of%20the%20checkerboard%20DNA-DNA%20hybridization%20technique%20for%20bacteria%20detection%20in%20Aedes%20aegypti.pdf.txt63e4d7f176997af5fd63b371a4bb1b39MD53icict/86922022-01-28 14:03:50.581oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-01-28T17:03:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
title |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
spellingShingle |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Gaio, Analiz de Oliveira Checkerboard DNA hybridization Aedes aegypti bacteria |
title_short |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
title_full |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
title_fullStr |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
title_full_unstemmed |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
title_sort |
Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). |
author |
Gaio, Analiz de Oliveira |
author_facet |
Gaio, Analiz de Oliveira Rodrigues, Rivea Cristina Custodio Nascimento, Cássio do Secundino, Nágila Francinete Costa Lemos, Francisco Jose Alves Pimenta, Paulo Filemon Paolucci Monesi, Nadia |
author_role |
author |
author2 |
Rodrigues, Rivea Cristina Custodio Nascimento, Cássio do Secundino, Nágila Francinete Costa Lemos, Francisco Jose Alves Pimenta, Paulo Filemon Paolucci Monesi, Nadia |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Gaio, Analiz de Oliveira Rodrigues, Rivea Cristina Custodio Nascimento, Cássio do Secundino, Nágila Francinete Costa Lemos, Francisco Jose Alves Pimenta, Paulo Filemon Paolucci Monesi, Nadia |
dc.subject.en.pt_BR.fl_str_mv |
Checkerboard DNA hybridization Aedes aegypti bacteria |
topic |
Checkerboard DNA hybridization Aedes aegypti bacteria |
description |
Universidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, Brazil |
publishDate |
2011 |
dc.date.issued.fl_str_mv |
2011 |
dc.date.accessioned.fl_str_mv |
2014-10-29T17:31:45Z |
dc.date.available.fl_str_mv |
2014-10-29T17:31:45Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/8692 |
dc.identifier.issn.none.fl_str_mv |
1756-3305 |
dc.identifier.doi.none.fl_str_mv |
10.1186/1756-3305-4-237 |
identifier_str_mv |
GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011. 1756-3305 10.1186/1756-3305-4-237 |
url |
https://www.arca.fiocruz.br/handle/icict/8692 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
dc.publisher.none.fl_str_mv |
BioMed Central |
publisher.none.fl_str_mv |
BioMed Central |
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