Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).

Detalhes bibliográficos
Autor(a) principal: Gaio, Analiz de Oliveira
Data de Publicação: 2011
Outros Autores: Rodrigues, Rivea Cristina Custodio, Nascimento, Cássio do, Secundino, Nágila Francinete Costa, Lemos, Francisco Jose Alves, Pimenta, Paulo Filemon Paolucci, Monesi, Nadia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8692
Resumo: Universidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, Brazil
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spelling Gaio, Analiz de OliveiraRodrigues, Rivea Cristina CustodioNascimento, Cássio doSecundino, Nágila Francinete CostaLemos, Francisco Jose AlvesPimenta, Paulo Filemon PaolucciMonesi, Nadia2014-10-29T17:31:45Z2014-10-29T17:31:45Z2011GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011.1756-3305https://www.arca.fiocruz.br/handle/icict/869210.1186/1756-3305-4-237engBioMed CentralUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilFaculdade de Odontologia de Ribeirão Preto. Departamento de Materiais Dentários e Prótese. Ribeirão Preto, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilUniversidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Entomologia Médica. Belo Horizonte, MG, BrazilFaculdade de Ciências Farmacêuticas de Ribeirão Preto. Departamento de Análises Clínicas, Toxicológicas e Bromatológicas. Ribeirão Preto, SP, BrazilBackground: Bacteria associated with insects can have a substantial impact on the biology and life cycle of their host. The checkerboard DNA-DNA hybridization technique is a semi-quantitative technique that has been previously employed in odontology to detect and quantify a variety of bacterial species in dental samples. Here we tested the applicability of the checkerboard DNA-DNA hybridization technique to detect the presence of Aedes aegypti-associated bacterial species in larvae, pupae and adults of A. aegypti. Findings: Using the checkerboard DNA-DNA hybridization technique we could detect and estimate the number of four bacterial species in total DNA samples extracted from A. aegypti single whole individuals and midguts. A. aegypti associated bacterial species were also detected in the midgut of four other insect species, Lutzomyia longipalpis, Drosophila melanogaster, Bradysia hygida andApis mellifera. Conclusions: Our results demonstrate that the checkerboard DNA-DNA hybridization technique can be employed to study the microbiota composition of mosquitoes. The method has the sensitivity to detect bacteria in single individuals, as well as in a single organ, and therefore can be employed to evaluate the differences in bacterial counts amongst individuals in a given mosquito population. We suggest that the checkerboard DNA-DNA hybridization technique is a straightforward technique that can be widely used for the characterization of the microbiota in mosquito populations.CheckerboardDNA hybridizationAedes aegyptibacteriainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8692/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdfUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdfapplication/pdf1405994https://www.arca.fiocruz.br/bitstream/icict/8692/2/Use%20of%20the%20checkerboard%20DNA-DNA%20hybridization%20technique%20for%20bacteria%20detection%20in%20Aedes%20aegypti.pdf8dfe54019468f8ccdcbcc2544c8c5b14MD52TEXTUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdf.txtUse of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti.pdf.txtExtracted texttext/plain19602https://www.arca.fiocruz.br/bitstream/icict/8692/3/Use%20of%20the%20checkerboard%20DNA-DNA%20hybridization%20technique%20for%20bacteria%20detection%20in%20Aedes%20aegypti.pdf.txt63e4d7f176997af5fd63b371a4bb1b39MD53icict/86922022-01-28 14:03:50.581oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-01-28T17:03:50Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
title Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
spellingShingle Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
Gaio, Analiz de Oliveira
Checkerboard
DNA hybridization
Aedes aegypti
bacteria
title_short Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
title_full Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
title_fullStr Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
title_full_unstemmed Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
title_sort Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.).
author Gaio, Analiz de Oliveira
author_facet Gaio, Analiz de Oliveira
Rodrigues, Rivea Cristina Custodio
Nascimento, Cássio do
Secundino, Nágila Francinete Costa
Lemos, Francisco Jose Alves
Pimenta, Paulo Filemon Paolucci
Monesi, Nadia
author_role author
author2 Rodrigues, Rivea Cristina Custodio
Nascimento, Cássio do
Secundino, Nágila Francinete Costa
Lemos, Francisco Jose Alves
Pimenta, Paulo Filemon Paolucci
Monesi, Nadia
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gaio, Analiz de Oliveira
Rodrigues, Rivea Cristina Custodio
Nascimento, Cássio do
Secundino, Nágila Francinete Costa
Lemos, Francisco Jose Alves
Pimenta, Paulo Filemon Paolucci
Monesi, Nadia
dc.subject.en.pt_BR.fl_str_mv Checkerboard
DNA hybridization
Aedes aegypti
bacteria
topic Checkerboard
DNA hybridization
Aedes aegypti
bacteria
description Universidade Estadual do Norte Fluminense. Laboratório de Biotecnologia Campos dos Goytacazes, RJ, Brazil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-29T17:31:45Z
dc.date.available.fl_str_mv 2014-10-29T17:31:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8692
dc.identifier.issn.none.fl_str_mv 1756-3305
dc.identifier.doi.none.fl_str_mv 10.1186/1756-3305-4-237
identifier_str_mv GAIO, Analiz de Oliveira et al. Use of the checkerboard DNA-DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L.). Parasites & Vectors, 4: 237, 2011.
1756-3305
10.1186/1756-3305-4-237
url https://www.arca.fiocruz.br/handle/icict/8692
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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