Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization

Detalhes bibliográficos
Autor(a) principal: Chahad-Ehlers, Samira
Data de Publicação: 2013
Outros Autores: Gentile, Carla, Lima, José Bento Pereira, Peixoto, Alexandre Afrânio, Bruno, Rafaela Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/11566
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.
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spelling Chahad-Ehlers, SamiraGentile, CarlaLima, José Bento PereiraPeixoto, Alexandre AfrânioBruno, Rafaela Vieira2015-08-19T13:49:33Z2015-08-19T13:49:33Z2013CHAHAD-EHLERS, Samira; et al. Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization. Plos One, v.8, n.1, 6p, jan. 2013.https://www.arca.fiocruz.br/handle/icict/1156610.1371/journal.pone.0052559engPlos OneAnalysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridizationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Fisiologia e Controle de Artrópodes Vetores. Rio de Janeiro, RJ, Brasil. Rio de Janeiro, RJ, Brasil / Laboratório de Entomologia. Instituto de Biologia do Exército. Rio de Janeiro, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, CNPq, Rio de Janeiro, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, CNPq, Rio de Janeiro, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil / Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular, CNPq, Rio de Janeiro, Brasil.Even though the blood-sucking mosquito Aedes aegypti is one of the most important disease vectors, relatively little is known about the molecular mechanisms underlying processes involved in the temporal pattern of its activity and host seeking behavior. In this study, we analyzed the expression of the cycle (cyc) gene, one of the core components of the circadian clock, in Ae. aegypti brains by in situ hybridization at two different time points in light-dark conditions and compared the results with those obtained using a quantitative PCR assay (qPCR). Within the brain, differential labeling was detected according to distinct areas empirically pre-defined. Six out of seven of these areas showed significantly higher staining at ZT3 (three hours after light-on) compared to ZT11 (one before light-off), which is consistent with the qPCR data. Predominant staining was observed in three of those areas which correspond to positions of the optical and antennal lobes, as well as the region where the neurons controlling activity rhythms are presumably localized.Aedes aegyptiAedes aegypti BrainsIn Situ HybridizationAnalysis of cycle Gene ExpressionAedesHibridização In SituEncéfaloinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11566/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALsamira_ehlers_etal_IOC_2013.pdfapplication/pdf287581https://www.arca.fiocruz.br/bitstream/icict/11566/2/samira_ehlers_etal_IOC_2013.pdf8e5f11fa5eb89fad2b4b95b764ca65faMD52TEXTsamira_ehlers_etal_IOC_2013.pdf.txtsamira_ehlers_etal_IOC_2013.pdf.txtExtracted texttext/plain36538https://www.arca.fiocruz.br/bitstream/icict/11566/3/samira_ehlers_etal_IOC_2013.pdf.txtce3f5ea092f8805f58bf151df0d40656MD53icict/115662022-06-24 13:02:47.668oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:02:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
title Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
spellingShingle Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
Chahad-Ehlers, Samira
Aedes aegypti
Aedes aegypti Brains
In Situ Hybridization
Analysis of cycle Gene Expression
Aedes
Hibridização In Situ
Encéfalo
title_short Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
title_full Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
title_fullStr Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
title_full_unstemmed Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
title_sort Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization
author Chahad-Ehlers, Samira
author_facet Chahad-Ehlers, Samira
Gentile, Carla
Lima, José Bento Pereira
Peixoto, Alexandre Afrânio
Bruno, Rafaela Vieira
author_role author
author2 Gentile, Carla
Lima, José Bento Pereira
Peixoto, Alexandre Afrânio
Bruno, Rafaela Vieira
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Chahad-Ehlers, Samira
Gentile, Carla
Lima, José Bento Pereira
Peixoto, Alexandre Afrânio
Bruno, Rafaela Vieira
dc.subject.en.pt_BR.fl_str_mv Aedes aegypti
Aedes aegypti Brains
In Situ Hybridization
Analysis of cycle Gene Expression
topic Aedes aegypti
Aedes aegypti Brains
In Situ Hybridization
Analysis of cycle Gene Expression
Aedes
Hibridização In Situ
Encéfalo
dc.subject.decs.pt_BR.fl_str_mv Aedes
Hibridização In Situ
Encéfalo
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Insetos. Rio de Janeiro, RJ, Brasil.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2015-08-19T13:49:33Z
dc.date.available.fl_str_mv 2015-08-19T13:49:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CHAHAD-EHLERS, Samira; et al. Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization. Plos One, v.8, n.1, 6p, jan. 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/11566
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0052559
identifier_str_mv CHAHAD-EHLERS, Samira; et al. Analysis of cycle Gene Expression in Aedes aegypti Brains by In Situ Hybridization. Plos One, v.8, n.1, 6p, jan. 2013.
10.1371/journal.pone.0052559
url https://www.arca.fiocruz.br/handle/icict/11566
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Plos One
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dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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