Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/36809 |
Resumo: | Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. |
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Pacheco, Rodrigo GoulartEsposito, Christiano CostaMüller, Lucas C. M.Castelo-Branco, Morgana T. L.Quintella, Leonardo PereiraChagas, Vera Lucia A.Souza, Heitor Siffert P. deSchanaider, Alberto2019-11-01T14:29:43Z2019-11-01T14:29:43Z2012PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012.1007-9327https://www.arca.fiocruz.br/handle/icict/3680910.3748/wjg.v18.i32.42782219-2840Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Laboratory of Cellular Immunology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Pathology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Pathology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Hospital Universitario Clementino Fraga Filho. Department of Internal Medicine. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.AIM: To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion colitis. METHODS: Wistar specific pathogen-free rats were submitted to a Hartmann’s end colostomy and treated with enemas containing glutamine, butyrate, or saline. Enemas were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical procedure. Follow-up colonoscopy was performed every 4 wk for 12 wk. The effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β, tumor necrosis factor-alpha, and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent assay. RESULTS: Colonoscopies of the diverted segment showed mucosa with hyperemia, increased number of vessels, bleeding and mucus discharge. Treatment with either glutamine or butyrate induced significant reductions in both colonoscopic (P < 0.02) and histological scores (P < 0.01) and restored the densities of collagen fibers in tissue (P = 0.015; P = 0.001), the number of goblet cells (P = 0.021; P = 0.029), and the rate of apoptosis within the epithelium (P = 0.043; P = 0.011) to normal values. The high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or glutamine. CONCLUSION: The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.engBaishideng Publishing GroupUse of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDiversion colitisButyrateGlutamineShortchain fatty acidsCytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36809/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Pacheco_Rodrigo_etal_INI_2012.pdfve_Pacheco_Rodrigo_etal_INI_2012.pdfapplication/pdf1293329https://www.arca.fiocruz.br/bitstream/icict/36809/2/ve_Pacheco_Rodrigo_etal_INI_2012.pdfce1721ea030772e327c899bdf406eb7bMD52TEXTve_Pacheco_Rodrigo_etal_INI_2012.pdf.txtve_Pacheco_Rodrigo_etal_INI_2012.pdf.txtExtracted texttext/plain46684https://www.arca.fiocruz.br/bitstream/icict/36809/3/ve_Pacheco_Rodrigo_etal_INI_2012.pdf.txt91f2d90f875e4e81a3eb71030f5ecc27MD53icict/368092019-11-02 02:00:47.568oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-11-02T05:00:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
dc.title.pt_BR.fl_str_mv |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
title |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
spellingShingle |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis Pacheco, Rodrigo Goulart Diversion colitis Butyrate Glutamine Shortchain fatty acids Cytokines |
title_short |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
title_full |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
title_fullStr |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
title_full_unstemmed |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
title_sort |
Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis |
author |
Pacheco, Rodrigo Goulart |
author_facet |
Pacheco, Rodrigo Goulart Esposito, Christiano Costa Müller, Lucas C. M. Castelo-Branco, Morgana T. L. Quintella, Leonardo Pereira Chagas, Vera Lucia A. Souza, Heitor Siffert P. de Schanaider, Alberto |
author_role |
author |
author2 |
Esposito, Christiano Costa Müller, Lucas C. M. Castelo-Branco, Morgana T. L. Quintella, Leonardo Pereira Chagas, Vera Lucia A. Souza, Heitor Siffert P. de Schanaider, Alberto |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Pacheco, Rodrigo Goulart Esposito, Christiano Costa Müller, Lucas C. M. Castelo-Branco, Morgana T. L. Quintella, Leonardo Pereira Chagas, Vera Lucia A. Souza, Heitor Siffert P. de Schanaider, Alberto |
dc.subject.en.pt_BR.fl_str_mv |
Diversion colitis Butyrate Glutamine Shortchain fatty acids Cytokines |
topic |
Diversion colitis Butyrate Glutamine Shortchain fatty acids Cytokines |
description |
Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento. |
publishDate |
2012 |
dc.date.issued.fl_str_mv |
2012 |
dc.date.accessioned.fl_str_mv |
2019-11-01T14:29:43Z |
dc.date.available.fl_str_mv |
2019-11-01T14:29:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/36809 |
dc.identifier.issn.pt_BR.fl_str_mv |
1007-9327 |
dc.identifier.doi.none.fl_str_mv |
10.3748/wjg.v18.i32.4278 |
dc.identifier.eissn.none.fl_str_mv |
2219-2840 |
identifier_str_mv |
PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012. 1007-9327 10.3748/wjg.v18.i32.4278 2219-2840 |
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https://www.arca.fiocruz.br/handle/icict/36809 |
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eng |
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Baishideng Publishing Group |
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Baishideng Publishing Group |
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