Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis

Detalhes bibliográficos
Autor(a) principal: Pacheco, Rodrigo Goulart
Data de Publicação: 2012
Outros Autores: Esposito, Christiano Costa, Müller, Lucas C. M., Castelo-Branco, Morgana T. L., Quintella, Leonardo Pereira, Chagas, Vera Lucia A., Souza, Heitor Siffert P. de, Schanaider, Alberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/36809
Resumo: Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
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spelling Pacheco, Rodrigo GoulartEsposito, Christiano CostaMüller, Lucas C. M.Castelo-Branco, Morgana T. L.Quintella, Leonardo PereiraChagas, Vera Lucia A.Souza, Heitor Siffert P. deSchanaider, Alberto2019-11-01T14:29:43Z2019-11-01T14:29:43Z2012PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012.1007-9327https://www.arca.fiocruz.br/handle/icict/3680910.3748/wjg.v18.i32.42782219-2840Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Institute of Biomedical Sciences. Department of Histology and Embryology. Laboratory of Cellular Immunology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Pathology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Pathology. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Hospital Universitario Clementino Fraga Filho. Department of Internal Medicine. Rio de Janeiro, RJ, Brasil.Federal University of Rio de Janeiro. Medical School. Department of Surgery. Experimental Surgery Center Surgical. Sciences Postgraduate Program. Rio de Janeiro, RJ, Brasil.AIM: To investigate whether butyrate or glutamine enemas could diminish inflammation in experimental diversion colitis. METHODS: Wistar specific pathogen-free rats were submitted to a Hartmann’s end colostomy and treated with enemas containing glutamine, butyrate, or saline. Enemas were administered twice a week in the excluded segment of the colon from 4 to 12 wk after the surgical procedure. Follow-up colonoscopy was performed every 4 wk for 12 wk. The effect of treatment was evaluated using video-endoscopic and histologic scores and measuring interleukin-1β, tumor necrosis factor-alpha, and transforming growth factor beta production in organ cultures by enzyme linked immunosorbent assay. RESULTS: Colonoscopies of the diverted segment showed mucosa with hyperemia, increased number of vessels, bleeding and mucus discharge. Treatment with either glutamine or butyrate induced significant reductions in both colonoscopic (P < 0.02) and histological scores (P < 0.01) and restored the densities of collagen fibers in tissue (P = 0.015; P = 0.001), the number of goblet cells (P = 0.021; P = 0.029), and the rate of apoptosis within the epithelium (P = 0.043; P = 0.011) to normal values. The high levels of cytokines in colon explants from rats with diversion colitis significantly decreased to normal values after treatment with butyrate or glutamine. CONCLUSION: The improvement of experimental diversion colitis following glutamine or butyrate enemas highlights the importance of specific luminal nutrients in the homeostasis of the colonic mucosa and supports their utilization for the treatment of human diversion colitis.engBaishideng Publishing GroupUse of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleDiversion colitisButyrateGlutamineShortchain fatty acidsCytokinesinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/36809/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Pacheco_Rodrigo_etal_INI_2012.pdfve_Pacheco_Rodrigo_etal_INI_2012.pdfapplication/pdf1293329https://www.arca.fiocruz.br/bitstream/icict/36809/2/ve_Pacheco_Rodrigo_etal_INI_2012.pdfce1721ea030772e327c899bdf406eb7bMD52TEXTve_Pacheco_Rodrigo_etal_INI_2012.pdf.txtve_Pacheco_Rodrigo_etal_INI_2012.pdf.txtExtracted texttext/plain46684https://www.arca.fiocruz.br/bitstream/icict/36809/3/ve_Pacheco_Rodrigo_etal_INI_2012.pdf.txt91f2d90f875e4e81a3eb71030f5ecc27MD53icict/368092019-11-02 02:00:47.568oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-11-02T05:00:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
title Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
spellingShingle Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
Pacheco, Rodrigo Goulart
Diversion colitis
Butyrate
Glutamine
Shortchain fatty acids
Cytokines
title_short Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
title_full Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
title_fullStr Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
title_full_unstemmed Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
title_sort Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis
author Pacheco, Rodrigo Goulart
author_facet Pacheco, Rodrigo Goulart
Esposito, Christiano Costa
Müller, Lucas C. M.
Castelo-Branco, Morgana T. L.
Quintella, Leonardo Pereira
Chagas, Vera Lucia A.
Souza, Heitor Siffert P. de
Schanaider, Alberto
author_role author
author2 Esposito, Christiano Costa
Müller, Lucas C. M.
Castelo-Branco, Morgana T. L.
Quintella, Leonardo Pereira
Chagas, Vera Lucia A.
Souza, Heitor Siffert P. de
Schanaider, Alberto
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pacheco, Rodrigo Goulart
Esposito, Christiano Costa
Müller, Lucas C. M.
Castelo-Branco, Morgana T. L.
Quintella, Leonardo Pereira
Chagas, Vera Lucia A.
Souza, Heitor Siffert P. de
Schanaider, Alberto
dc.subject.en.pt_BR.fl_str_mv Diversion colitis
Butyrate
Glutamine
Shortchain fatty acids
Cytokines
topic Diversion colitis
Butyrate
Glutamine
Shortchain fatty acids
Cytokines
description Leonardo Pereira Quintella. Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta a informação no documento.
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2019-11-01T14:29:43Z
dc.date.available.fl_str_mv 2019-11-01T14:29:43Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/36809
dc.identifier.issn.pt_BR.fl_str_mv 1007-9327
dc.identifier.doi.none.fl_str_mv 10.3748/wjg.v18.i32.4278
dc.identifier.eissn.none.fl_str_mv 2219-2840
identifier_str_mv PACHECO, Rodrigo Goulart et al. Use of butyrate or glutamine in enema solution reduces inflammation and fibrosis in experimental diversion colitis. World Journal of Gastroenterology, v. 18, n. 32, p. 4278-4287, Aug. 2012.
1007-9327
10.3748/wjg.v18.i32.4278
2219-2840
url https://www.arca.fiocruz.br/handle/icict/36809
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Baishideng Publishing Group
publisher.none.fl_str_mv Baishideng Publishing Group
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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