Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies

Detalhes bibliográficos
Autor(a) principal: Amado, Luciane A.
Data de Publicação: 2006
Outros Autores: Villar, Livia M., Paula, Vanessa S. de, Almeida, Adilson J. de, Gaspar, Ana Maria C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/38720
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.
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spelling Amado, Luciane A.Villar, Livia M.Paula, Vanessa S. deAlmeida, Adilson J. deGaspar, Ana Maria C.2019-12-19T16:49:50Z2019-12-19T16:49:50Z2006AMADO, Luciana A. et al. Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 2, p. 149-165, Mar. 2006.0074-0206https://www.arca.fiocruz.br/handle/icict/3872010.1590/s0074-027620060002000061678-8060engFluido oralHepatites viraisDiagnósticoEpidemiologiaOral fluidViral hepatitisDiagnostic and epidemiologyDetection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studiesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.In this report, we examine the adaptability of commercially available serological kits to detect antibodies markers for viral hepatitis in oral fluid samples. We also assessed the prevalence of hepatitis A, B, and C virus-specific antibodies, and related risk factors for these infectious diseases through sensitivity of the tests in saliva samples to evaluate if oralfluid can be an alternative tool to substitute serum in diagnosis of acute viral hepatitis and in epidemiological studies. One hundred and ten paired serum and saliva specimens from suspect patients of having acute hepatitis were collected to detect antibodies to hepatitis A (total and IgM), hepatitis B (anti-HBs, total anti-HBc and IgM anti-HBc), and hepatitis C (anti-HCV) using commercially available enzyme-linked immunossorbent assay (EIA). In relation to serum samples, oral fluid assay sensitivity and specificity were as follows: 87 and 100% for total anti-HAV, 79 and 100% for anti-HAVIgM, 6 and 95% for anti-HBs, 13 and 100%for total anti-HBc, 100 and 100% for anti-HBc IgM, and 75 and 100% for anti-HCV The consistency observed between antibodies tests in saliva and expected risk factors for hepatitis A and C suggests that the saliva method could replace serum in epidemiological studies for hepatitis A and C.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
title Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
spellingShingle Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
Amado, Luciane A.
Fluido oral
Hepatites virais
Diagnóstico
Epidemiologia
Oral fluid
Viral hepatitis
Diagnostic and epidemiology
title_short Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
title_full Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
title_fullStr Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
title_full_unstemmed Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
title_sort Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies
author Amado, Luciane A.
author_facet Amado, Luciane A.
Villar, Livia M.
Paula, Vanessa S. de
Almeida, Adilson J. de
Gaspar, Ana Maria C.
author_role author
author2 Villar, Livia M.
Paula, Vanessa S. de
Almeida, Adilson J. de
Gaspar, Ana Maria C.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Amado, Luciane A.
Villar, Livia M.
Paula, Vanessa S. de
Almeida, Adilson J. de
Gaspar, Ana Maria C.
dc.subject.other.pt_BR.fl_str_mv Fluido oral
Hepatites virais
Diagnóstico
Epidemiologia
topic Fluido oral
Hepatites virais
Diagnóstico
Epidemiologia
Oral fluid
Viral hepatitis
Diagnostic and epidemiology
dc.subject.en.pt_BR.fl_str_mv Oral fluid
Viral hepatitis
Diagnostic and epidemiology
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Laboratório de Desenvolvimento Tecnológico. Rio de Janeiro, RJ, Brasil.
publishDate 2006
dc.date.issued.fl_str_mv 2006
dc.date.accessioned.fl_str_mv 2019-12-19T16:49:50Z
dc.date.available.fl_str_mv 2019-12-19T16:49:50Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv AMADO, Luciana A. et al. Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 2, p. 149-165, Mar. 2006.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/38720
dc.identifier.issn.pt_BR.fl_str_mv 0074-0206
dc.identifier.doi.none.fl_str_mv 10.1590/s0074-02762006000200006
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv AMADO, Luciana A. et al. Detection of hepatitis A, B, and C virus-specific antibodies using oral fluid for epidemiological studies. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 101, n. 2, p. 149-165, Mar. 2006.
0074-0206
10.1590/s0074-02762006000200006
1678-8060
url https://www.arca.fiocruz.br/handle/icict/38720
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/38720/2/VanessaSPaula_AnaMGaspar_etal_IOC_2006.pdf
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