Combined methods to evaluate human cells in muscle xenografts

Detalhes bibliográficos
Autor(a) principal: Bensalah, Mona
Data de Publicação: 2019
Outros Autores: Klein, Pierre, Riederer, Ingo, Chaouch, Soraya, Muraine, Laura, Savino, Wilson, Butler-Browne, Gillian Sandra, Trollet, Capucine, Mouly, Vincent, Bigot, Anne
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/33839
Resumo: Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.
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spelling Bensalah, MonaKlein, PierreRiederer, IngoChaouch, SorayaMuraine, LauraSavino, WilsonButler-Browne, Gillian SandraTrollet, CapucineMouly, VincentBigot, Anne2019-07-04T14:58:22Z2019-07-04T14:58:22Z2019BENSALAH, Mona et al. Combined methods to evaluate human cells in muscle xenografts. Plos One, v. 14, n. 5, p. 1-12, May 2019.1932-6203https://www.arca.fiocruz.br/handle/icict/3383910.1371/journal.pone.0211522engPublic Library of ScienceMétodos combinadosXenógrafos de músculosCélulas humanasXenotransplanteCombined methodsMuscle xenograftsHuman cellsXenotransplantationCombined methods to evaluate human cells in muscle xenograftsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleSorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional em Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional em Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.Xenotransplantation of human cells into immunodeficient mouse models is a very powerful tool and an essential step for the pre-clinical evaluation of therapeutic cell- and gene- based strategies. Here we describe an optimized protocol combining immunofluorescence and real-time quantitative PCR to both quantify and visualize the fate and localization of human myogenic cells after injection in regenerating muscles of immunodeficient mice. Whereas real-time quantitative PCR-based method provides an accurate quantification of human cells, it does not document their specific localization. The addition of an immunofluorescence approach using human-specific antibodies recognizing engrafted human cells gives information on the localization of the human cells within the host muscle fibres, in the stem cell niche or in the interstitial space. These two combined approaches offer an accurate evaluation of human engraftment including cell number and localization and should provide a gold standard to compare results obtained either using different types of human stem cells or comparing healthy and pathological muscle stem cells between different research laboratories worldwide.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/33839/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALWilsonSavino_etal_IOC_2019.pdfWilsonSavino_etal_IOC_2019.pdfapplication/pdf1640556https://www.arca.fiocruz.br/bitstream/icict/33839/2/WilsonSavino_etal_IOC_2019.pdf9f7bb28451c370473f8d076dc5898d16MD52TEXTWilsonSavino_etal_IOC_2019.pdf.txtWilsonSavino_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Combined methods to evaluate human cells in muscle xenografts
title Combined methods to evaluate human cells in muscle xenografts
spellingShingle Combined methods to evaluate human cells in muscle xenografts
Bensalah, Mona
Métodos combinados
Xenógrafos de músculos
Células humanas
Xenotransplante
Combined methods
Muscle xenografts
Human cells
Xenotransplantation
title_short Combined methods to evaluate human cells in muscle xenografts
title_full Combined methods to evaluate human cells in muscle xenografts
title_fullStr Combined methods to evaluate human cells in muscle xenografts
title_full_unstemmed Combined methods to evaluate human cells in muscle xenografts
title_sort Combined methods to evaluate human cells in muscle xenografts
author Bensalah, Mona
author_facet Bensalah, Mona
Klein, Pierre
Riederer, Ingo
Chaouch, Soraya
Muraine, Laura
Savino, Wilson
Butler-Browne, Gillian Sandra
Trollet, Capucine
Mouly, Vincent
Bigot, Anne
author_role author
author2 Klein, Pierre
Riederer, Ingo
Chaouch, Soraya
Muraine, Laura
Savino, Wilson
Butler-Browne, Gillian Sandra
Trollet, Capucine
Mouly, Vincent
Bigot, Anne
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bensalah, Mona
Klein, Pierre
Riederer, Ingo
Chaouch, Soraya
Muraine, Laura
Savino, Wilson
Butler-Browne, Gillian Sandra
Trollet, Capucine
Mouly, Vincent
Bigot, Anne
dc.subject.other.pt_BR.fl_str_mv Métodos combinados
Xenógrafos de músculos
Células humanas
Xenotransplante
topic Métodos combinados
Xenógrafos de músculos
Células humanas
Xenotransplante
Combined methods
Muscle xenografts
Human cells
Xenotransplantation
dc.subject.en.pt_BR.fl_str_mv Combined methods
Muscle xenografts
Human cells
Xenotransplantation
description Sorbonne Université. Myology Research Center. UM76 and INSERM U974. Institut de Myologie. Paris, France.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-07-04T14:58:22Z
dc.date.available.fl_str_mv 2019-07-04T14:58:22Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BENSALAH, Mona et al. Combined methods to evaluate human cells in muscle xenografts. Plos One, v. 14, n. 5, p. 1-12, May 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/33839
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0211522
identifier_str_mv BENSALAH, Mona et al. Combined methods to evaluate human cells in muscle xenografts. Plos One, v. 14, n. 5, p. 1-12, May 2019.
1932-6203
10.1371/journal.pone.0211522
url https://www.arca.fiocruz.br/handle/icict/33839
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/33839/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/33839/2/WilsonSavino_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/33839/3/WilsonSavino_etal_IOC_2019.pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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