Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Pesquisa Veterinária Brasileira (Online) |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425 |
Resumo: | ABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures. |
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Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenograftsXenotransplantationovaryheifersmiceABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.Colégio Brasileiro de Patologia Animal - CBPA2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425Pesquisa Veterinária Brasileira v.37 n.5 2017reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/s0100-736x2017000500001info:eu-repo/semantics/openAccessPimentel,Muriel M.L.Santos,Fernanda A.Teixeira,Ana C.G.Izzo,Roberta G.Lima,Mikael A.Macedo,Michelly F.Bezerra,Marcelo B.eng2017-08-29T00:00:00Zoai:scielo:S0100-736X2017000500425Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2017-08-29T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false |
dc.title.none.fl_str_mv |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
title |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
spellingShingle |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts Pimentel,Muriel M.L. Xenotransplantation ovary heifers mice |
title_short |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
title_full |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
title_fullStr |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
title_full_unstemmed |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
title_sort |
Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts |
author |
Pimentel,Muriel M.L. |
author_facet |
Pimentel,Muriel M.L. Santos,Fernanda A. Teixeira,Ana C.G. Izzo,Roberta G. Lima,Mikael A. Macedo,Michelly F. Bezerra,Marcelo B. |
author_role |
author |
author2 |
Santos,Fernanda A. Teixeira,Ana C.G. Izzo,Roberta G. Lima,Mikael A. Macedo,Michelly F. Bezerra,Marcelo B. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Pimentel,Muriel M.L. Santos,Fernanda A. Teixeira,Ana C.G. Izzo,Roberta G. Lima,Mikael A. Macedo,Michelly F. Bezerra,Marcelo B. |
dc.subject.por.fl_str_mv |
Xenotransplantation ovary heifers mice |
topic |
Xenotransplantation ovary heifers mice |
description |
ABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-05-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/s0100-736x2017000500001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
publisher.none.fl_str_mv |
Colégio Brasileiro de Patologia Animal - CBPA |
dc.source.none.fl_str_mv |
Pesquisa Veterinária Brasileira v.37 n.5 2017 reponame:Pesquisa Veterinária Brasileira (Online) instname:Colégio Brasileiro de Patologia Animal (CBPA) instacron:EMBRAPA |
instname_str |
Colégio Brasileiro de Patologia Animal (CBPA) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Pesquisa Veterinária Brasileira (Online) |
collection |
Pesquisa Veterinária Brasileira (Online) |
repository.name.fl_str_mv |
Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA) |
repository.mail.fl_str_mv |
colegio@cbpa.org.br||pvb@pvb.com.br |
_version_ |
1754122237017849856 |