Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts

Detalhes bibliográficos
Autor(a) principal: Pimentel,Muriel M.L.
Data de Publicação: 2017
Outros Autores: Santos,Fernanda A., Teixeira,Ana C.G., Izzo,Roberta G., Lima,Mikael A., Macedo,Michelly F., Bezerra,Marcelo B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Pesquisa Veterinária Brasileira (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425
Resumo: ABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.
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spelling Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenograftsXenotransplantationovaryheifersmiceABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.Colégio Brasileiro de Patologia Animal - CBPA2017-05-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425Pesquisa Veterinária Brasileira v.37 n.5 2017reponame:Pesquisa Veterinária Brasileira (Online)instname:Colégio Brasileiro de Patologia Animal (CBPA)instacron:EMBRAPA10.1590/s0100-736x2017000500001info:eu-repo/semantics/openAccessPimentel,Muriel M.L.Santos,Fernanda A.Teixeira,Ana C.G.Izzo,Roberta G.Lima,Mikael A.Macedo,Michelly F.Bezerra,Marcelo B.eng2017-08-29T00:00:00Zoai:scielo:S0100-736X2017000500425Revistahttp://www.pvb.com.br/https://old.scielo.br/oai/scielo-oai.phpcolegio@cbpa.org.br||pvb@pvb.com.br0100-736X1678-5150opendoar:2017-08-29T00:00Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)false
dc.title.none.fl_str_mv Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
title Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
spellingShingle Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
Pimentel,Muriel M.L.
Xenotransplantation
ovary
heifers
mice
title_short Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
title_full Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
title_fullStr Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
title_full_unstemmed Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
title_sort Biochemical, thermographic, and follicular responses of murine models of hormone-treated bovine ovarian renal capsule xenografts
author Pimentel,Muriel M.L.
author_facet Pimentel,Muriel M.L.
Santos,Fernanda A.
Teixeira,Ana C.G.
Izzo,Roberta G.
Lima,Mikael A.
Macedo,Michelly F.
Bezerra,Marcelo B.
author_role author
author2 Santos,Fernanda A.
Teixeira,Ana C.G.
Izzo,Roberta G.
Lima,Mikael A.
Macedo,Michelly F.
Bezerra,Marcelo B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pimentel,Muriel M.L.
Santos,Fernanda A.
Teixeira,Ana C.G.
Izzo,Roberta G.
Lima,Mikael A.
Macedo,Michelly F.
Bezerra,Marcelo B.
dc.subject.por.fl_str_mv Xenotransplantation
ovary
heifers
mice
topic Xenotransplantation
ovary
heifers
mice
description ABSTRACT: This study aimed to evaluate the characteristics of two different murine models of hormone-treated renal-encapsulated bovine ovarian tissue xenotransplantation. Two immunodeficient mouse models (BALB/c Nude and C57BL6 SCID) were xenografted with ovarian pieces from heifers and each group was subjected to two hormonal treatments of eCG or a combination of FSH+LH. Donor ovaries and recipients were evaluated by histology and infrared thermography at different times. At the time of xenograft collection, animals were evaluated for alterations in hepatorenal biochemistry. The statistical test used in the study was ANOVA, followed by Tukey’s test. Among the strains, 80% of C57BL6 SCID and 77% of BALB/c Nude mice showed development and vascularization of the transplanted tissue, which acquired cyclicity at 19 and 9 days post-transplant, respectively. Hemorrhagic follicles in xenografts induced with FSH+LH were found in the C57BL6 SCID strain. Infrared thermography was insufficient to distinguish the tissue donor recipient. In conclusion, the C57BL6 SCID strain appears to be the best host for ovarian xenografts, since the transplants in these mice were viable and showed robust follicular development. This work will aid future choices of immunodeficient strains for xenografting procedures.
publishDate 2017
dc.date.none.fl_str_mv 2017-05-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-736X2017000500425
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/s0100-736x2017000500001
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
publisher.none.fl_str_mv Colégio Brasileiro de Patologia Animal - CBPA
dc.source.none.fl_str_mv Pesquisa Veterinária Brasileira v.37 n.5 2017
reponame:Pesquisa Veterinária Brasileira (Online)
instname:Colégio Brasileiro de Patologia Animal (CBPA)
instacron:EMBRAPA
instname_str Colégio Brasileiro de Patologia Animal (CBPA)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Pesquisa Veterinária Brasileira (Online)
collection Pesquisa Veterinária Brasileira (Online)
repository.name.fl_str_mv Pesquisa Veterinária Brasileira (Online) - Colégio Brasileiro de Patologia Animal (CBPA)
repository.mail.fl_str_mv colegio@cbpa.org.br||pvb@pvb.com.br
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