An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/36742 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
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Reis, Sônia Regina Nogueira IgnácioSampaio, André Luiz FrancoHenriques, Maria das Graças MullerGandini, MarianaAzeredo, Elzinandes LealKubelka, Claire Fernandes2019-10-29T14:02:20Z2019-10-29T14:02:20Z2007REIS, Sônia Regina Nogueira Ignácio et al. An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 8, p. 983-990, Dec. 2007.0074-0276https://www.arca.fiocruz.br/handle/icict/3674210.1590/s0074-027620070008000141678-8060engFundação Oswaldo Cruz. Instituto Oswaldo CruzDengueCitocinasMonócitosTerapêuticaDexametasonaDengueDexamethasoneMonocytesCytokinesTherapeuticsAn in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Departamento de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Farmanguinhos. Departamento de Farmacologia Aplicada. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.An important cytokine role in dengue fever pathogenesis has been described. These molecules can be associated with haemorrhagic manifestations, coagulation disorders, hypotension and shock, all symptoms implicated in vascular permeability and disease worsening conditions. Several immunological diseases have been treated by cytokine modulation and dexamethasone is utilized clinically to treat pathologies with inflammatory and autoimmune etiologies. We established an in vitro model with human monocytes infected by dengue virus-2 for evaluating immunomodulatory and antiviral activities of potential pharmaceutical products. Flow cytometry analysis demonstrated significant dengue antigen detection in target cells two days after infection. TNF-alpha, IFN-alpha, IL-6 and IL-10 are produced by in vitro infected monocytes and are significantly detected in cell culture supernatants by multiplex microbead immunoassay. Dexamethasone action was tested for the first time for its modulation in dengue infection, presenting optimistic results in both decreasing cell infection rates and inhibiting TNF-alpha, IFN-alpha and IL-10 production. This model is proposed for novel drug trials yet to be applied for dengue fever.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/36742/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdfSoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdfapplication/pdf437848https://www.arca.fiocruz.br/bitstream/icict/36742/2/SoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdfca87862913a9d9890f95ad406d58b0e8MD52TEXTSoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdf.txtSoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdf.txtExtracted texttext/plain38073https://www.arca.fiocruz.br/bitstream/icict/36742/3/SoniaReginaReis_ClaireKubelka_etal_IOC_2007.pdf.txtdf327a64b5847c665b17c5d111ba5834MD53icict/367422019-11-26 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| dc.title.pt_BR.fl_str_mv |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| title |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| spellingShingle |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation Reis, Sônia Regina Nogueira Ignácio Dengue Citocinas Monócitos Terapêutica Dexametasona Dengue Dexamethasone Monocytes Cytokines Therapeutics |
| title_short |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| title_full |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| title_fullStr |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| title_full_unstemmed |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| title_sort |
An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation |
| author |
Reis, Sônia Regina Nogueira Ignácio |
| author_facet |
Reis, Sônia Regina Nogueira Ignácio Sampaio, André Luiz Franco Henriques, Maria das Graças Muller Gandini, Mariana Azeredo, Elzinandes Leal Kubelka, Claire Fernandes |
| author_role |
author |
| author2 |
Sampaio, André Luiz Franco Henriques, Maria das Graças Muller Gandini, Mariana Azeredo, Elzinandes Leal Kubelka, Claire Fernandes |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Reis, Sônia Regina Nogueira Ignácio Sampaio, André Luiz Franco Henriques, Maria das Graças Muller Gandini, Mariana Azeredo, Elzinandes Leal Kubelka, Claire Fernandes |
| dc.subject.other.pt_BR.fl_str_mv |
Dengue Citocinas Monócitos Terapêutica Dexametasona |
| topic |
Dengue Citocinas Monócitos Terapêutica Dexametasona Dengue Dexamethasone Monocytes Cytokines Therapeutics |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Dexamethasone Monocytes Cytokines Therapeutics |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2007 |
| dc.date.issued.fl_str_mv |
2007 |
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2019-10-29T14:02:20Z |
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2019-10-29T14:02:20Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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REIS, Sônia Regina Nogueira Ignácio et al. An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 8, p. 983-990, Dec. 2007. |
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https://www.arca.fiocruz.br/handle/icict/36742 |
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0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/s0074-02762007000800014 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
REIS, Sônia Regina Nogueira Ignácio et al. An in vitro model for dengue virus infection that exhibits human monocyte infection, multiple cytokine production and dexamethasone immunomodulation. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 102, n. 8, p. 983-990, Dec. 2007. 0074-0276 10.1590/s0074-02762007000800014 1678-8060 |
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eng |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz |
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