Dengue-2 infection and the induction of apoptosis in human primary monocytes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2009 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/28985 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
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Torrentes-Carvalho, AmandaAzeredo, Elzinandes L.Reis, Sonia R. I.Miranda, Alessandro S.Gandini, MarianaBarbosa, Luciana S.Kubelka, Claire F.2018-09-22T16:10:10Z2018-09-22T16:10:10Z2009TORRENTES-CARVALHO, Amanda; et al. Dengue-2 infection and the induction of apoptosis in human primary monocytes. Mem Inst Oswaldo Cruz, v.104, n.8, p.1091-1099, Dec. 2009 .0074-0276https://www.arca.fiocruz.br/handle/icict/289851678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.DengueApoptosemonócitoshumanosDengueApoptosismonocyteshumanDengue-2 infection and the induction of apoptosis in human primary monocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Monocytes/macrophages are important targets for dengue virus (DENV) replication; they induce inflammatory mediators and are sources of viral dissemination in the initial phase of the disease. Apoptosis is an active process of cellular destruction genetically regulated, in which a complex enzymatic pathway is activated and may be trigged by many viral infections. Since the mechanisms of apoptotic induction in DENV-infected target cells are not yet defined, we investigated the virus-cell interaction using a model of primary human monocyte infection with DENV-2 with the aim of identifying apoptotic markers. Cultures analyzed by flow cytometry and confocal microscopy yielded DENV antigen positive cells with rates that peaked at the second day post infection (p.i.), decayed afterwards and produced the apoptosis-related cytokines TNF-α and IL-10. Phosphatidylserine, an early marker for apoptosis, was increased at the cell surface and the Fas death receptor was upregulated at the second day p.i. at significantly higher rates in DENV infected cell cultures than controls. However, no detectable changes were observed in the expression of the anti-apoptotic protein Bcl-2 in infected cultures. Our data support virus modulation of extrinsic apoptotic factors in the in vitro model of human monocyte DENV-2 infection. DENV may be interfering in activation and death mechanisms by inducing apoptosis in target cells.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/28985/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALamanda_torres_etal_IOC_2009.pdfamanda_torres_etal_IOC_2009.pdfapplication/pdf554000https://www.arca.fiocruz.br/bitstream/icict/28985/2/amanda_torres_etal_IOC_2009.pdf2af8143ce27df129ad03be2dc7e3827bMD52TEXTamanda_torres_etal_IOC_2009.pdf.txtamanda_torres_etal_IOC_2009.pdf.txtExtracted texttext/plain47777https://www.arca.fiocruz.br/bitstream/icict/28985/3/amanda_torres_etal_IOC_2009.pdf.txt1cbfb8f98b59c2e0a719f28236cb564bMD53icict/289852018-09-23 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| dc.title.pt_BR.fl_str_mv |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| title |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| spellingShingle |
Dengue-2 infection and the induction of apoptosis in human primary monocytes Torrentes-Carvalho, Amanda Dengue Apoptose monócitos humanos Dengue Apoptosis monocytes human |
| title_short |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| title_full |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| title_fullStr |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| title_full_unstemmed |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| title_sort |
Dengue-2 infection and the induction of apoptosis in human primary monocytes |
| author |
Torrentes-Carvalho, Amanda |
| author_facet |
Torrentes-Carvalho, Amanda Azeredo, Elzinandes L. Reis, Sonia R. I. Miranda, Alessandro S. Gandini, Mariana Barbosa, Luciana S. Kubelka, Claire F. |
| author_role |
author |
| author2 |
Azeredo, Elzinandes L. Reis, Sonia R. I. Miranda, Alessandro S. Gandini, Mariana Barbosa, Luciana S. Kubelka, Claire F. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Torrentes-Carvalho, Amanda Azeredo, Elzinandes L. Reis, Sonia R. I. Miranda, Alessandro S. Gandini, Mariana Barbosa, Luciana S. Kubelka, Claire F. |
| dc.subject.other.pt_BR.fl_str_mv |
Dengue Apoptose monócitos humanos |
| topic |
Dengue Apoptose monócitos humanos Dengue Apoptosis monocytes human |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Apoptosis monocytes human |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2009 |
| dc.date.issued.fl_str_mv |
2009 |
| dc.date.accessioned.fl_str_mv |
2018-09-22T16:10:10Z |
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2018-09-22T16:10:10Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
TORRENTES-CARVALHO, Amanda; et al. Dengue-2 infection and the induction of apoptosis in human primary monocytes. Mem Inst Oswaldo Cruz, v.104, n.8, p.1091-1099, Dec. 2009 . |
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https://www.arca.fiocruz.br/handle/icict/28985 |
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0074-0276 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
TORRENTES-CARVALHO, Amanda; et al. Dengue-2 infection and the induction of apoptosis in human primary monocytes. Mem Inst Oswaldo Cruz, v.104, n.8, p.1091-1099, Dec. 2009 . 0074-0276 1678-8060 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
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