Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic

Detalhes bibliográficos
Autor(a) principal: Pagani, Talita Duarte
Data de Publicação: 2019
Outros Autores: Guimarães, Ana Carolina R., Waghabi, Mariana C., Corrêa, Paloma Rezende, Kalume, Dário Eluan, Berrêdo-Pinho, Marcia, Degrave, Wim Maurits, Lima, Leila Mendonça
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/35288
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
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spelling Pagani, Talita DuarteGuimarães, Ana Carolina R.Waghabi, Mariana C.Corrêa, Paloma RezendeKalume, Dário EluanBerrêdo-Pinho, MarciaDegrave, Wim MauritsLima, Leila Mendonça2019-09-03T12:32:35Z2019-09-03T12:32:35Z2019PAGANI, Talita Duarte et al. Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic. Frontiers in Immunology, v. 10, Article 716, 17p, Apr. 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/3528810.3389/fimmu.2019.00716engFrontiers MediaProteínas associadas à superfícieEletroforese bidimensionalMycobacterium bovisMycobacterium bovis BCG MoreauBi-dimensional electrophoresis,MALDI-TOF-TOFSurface- associated proteinsMoonlighting proteinsExploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomicinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório Interdisciplinar de Pesquisas Médicas. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Bioquímica Médica Leopoldo de Meis. Unidade de Espectometria de Massas e Proteômica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Microbiologia Celular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.Surface-associated proteins from Mycobacterium bovis BCG Moreau RDJ are important components of the live Brazilian vaccine against tuberculosis. They are important targets during initial BCG vaccine stimulation and modulation of the host's immune response, especially in the bacterial-host interaction. These proteins might also be involved in cellular communication, chemical response to the environment, pathogenesis processes through mobility, colonization, and adherence to the host cell, therefore performing multiple functions. In this study, the proteomic profile of the surface-associated proteins from M. bovis BCG Moreau was compared to the BCG Pasteur reference strain. The methodology used was 2DE gel electrophoresis combined with mass spectrometry techniques (MALDI-TOF/TOF), leading to the identification of 115 proteins. Of these, 24 proteins showed differential expression between the two BCG strains. Furthermore, 27 proteins previously described as displaying moonlighting function were identified, 8 of these proteins showed variation in abundance comparing BCG Moreau to Pasteur and 2 of them presented two different domain hits. Moonlighting proteins are multifunctional proteins in which two or more biological functions are fulfilled by a single polypeptide chain. Therefore, the identification of such proteins with moonlighting predicted functions can contribute to a better understanding of the molecular mechanisms unleashed by live BCG Moreau RDJ vaccine components.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35288/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALTalitaPagani_LeilaMLima_etal_IOC_2019.pdfTalitaPagani_LeilaMLima_etal_IOC_2019.pdfapplication/pdf2490553https://www.arca.fiocruz.br/bitstream/icict/35288/2/TalitaPagani_LeilaMLima_etal_IOC_2019.pdfbec0e4e17098191a9db8299c1d950da1MD52TEXTTalitaPagani_LeilaMLima_etal_IOC_2019.pdf.txtTalitaPagani_LeilaMLima_etal_IOC_2019.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
title Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
spellingShingle Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
Pagani, Talita Duarte
Proteínas associadas à superfície
Eletroforese bidimensional
Mycobacterium bovis
Mycobacterium bovis BCG Moreau
Bi-dimensional electrophoresis,
MALDI-TOF-TOF
Surface- associated proteins
Moonlighting proteins
title_short Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
title_full Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
title_fullStr Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
title_full_unstemmed Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
title_sort Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic
author Pagani, Talita Duarte
author_facet Pagani, Talita Duarte
Guimarães, Ana Carolina R.
Waghabi, Mariana C.
Corrêa, Paloma Rezende
Kalume, Dário Eluan
Berrêdo-Pinho, Marcia
Degrave, Wim Maurits
Lima, Leila Mendonça
author_role author
author2 Guimarães, Ana Carolina R.
Waghabi, Mariana C.
Corrêa, Paloma Rezende
Kalume, Dário Eluan
Berrêdo-Pinho, Marcia
Degrave, Wim Maurits
Lima, Leila Mendonça
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pagani, Talita Duarte
Guimarães, Ana Carolina R.
Waghabi, Mariana C.
Corrêa, Paloma Rezende
Kalume, Dário Eluan
Berrêdo-Pinho, Marcia
Degrave, Wim Maurits
Lima, Leila Mendonça
dc.subject.other.pt_BR.fl_str_mv Proteínas associadas à superfície
Eletroforese bidimensional
Mycobacterium bovis
topic Proteínas associadas à superfície
Eletroforese bidimensional
Mycobacterium bovis
Mycobacterium bovis BCG Moreau
Bi-dimensional electrophoresis,
MALDI-TOF-TOF
Surface- associated proteins
Moonlighting proteins
dc.subject.en.pt_BR.fl_str_mv Mycobacterium bovis BCG Moreau
Bi-dimensional electrophoresis,
MALDI-TOF-TOF
Surface- associated proteins
Moonlighting proteins
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genômica Funcional e Bioinformática. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-09-03T12:32:35Z
dc.date.available.fl_str_mv 2019-09-03T12:32:35Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PAGANI, Talita Duarte et al. Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic. Frontiers in Immunology, v. 10, Article 716, 17p, Apr. 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/35288
dc.identifier.issn.pt_BR.fl_str_mv 1664-3224
dc.identifier.doi.none.fl_str_mv 10.3389/fimmu.2019.00716
identifier_str_mv PAGANI, Talita Duarte et al. Exploring the Potential Role of Moonlighting Function of the Surface-Associated Proteins From Mycobacterium bovis BCG Moreau and Pasteur by Comparative Proteomic. Frontiers in Immunology, v. 10, Article 716, 17p, Apr. 2019.
1664-3224
10.3389/fimmu.2019.00716
url https://www.arca.fiocruz.br/handle/icict/35288
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/35288/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/35288/2/TalitaPagani_LeilaMLima_etal_IOC_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/35288/3/TalitaPagani_LeilaMLima_etal_IOC_2019.pdf.txt
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