Trypanosoma cruzi gene expression in response to gamma radiation

Detalhes bibliográficos
Autor(a) principal: Grynberg, Priscila
Data de Publicação: 2012
Outros Autores: Silva, Danielle Gomes Passos, Mourão, Marina de Moraes, Hirata Junior, Roberto, Macedo, Andrea Mara, Machado, Carlos Renato, Bartholomeu, Daniella Castanheira, Franco, Glória Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7593
Resumo: Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
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spelling Grynberg, PriscilaSilva, Danielle Gomes PassosMourão, Marina de MoraesHirata Junior, RobertoMacedo, Andrea MaraMachado, Carlos RenatoBartholomeu, Daniella CastanheiraFranco, Glória Regina2014-05-08T18:39:52Z2014-05-08T18:39:52Z2012GRYNBERG, Priscila et al. Trypanosoma cruzi gene expression in response to gamma radiation. Plos One. 2012, vol.7, pp. e29596.https://www.arca.fiocruz.br/handle/icict/759310.1371/journal.pone.0029596engPublic Library Of ScienceTrypanosoma cruzi gene expression in response to gamma radiationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo de Genômica e Biologia Computacional. Belo Horizonte, MG, Brazil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciência e Tecnologia em Doenças Tropicais Belo Horizonte, MG, BrazilUniversidade de São Paulo. Instituto de Matemática e Estatística. São Paulo, SP, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Parasitologia. Belo Horizonte, MG, BrazilUniversidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, BrazilTrypanosoma cruzi is an organism highly resistant to ionizing radiation. Following a dose of 500 Gy of gamma radiation, the fragmented genomic DNA is gradually reconstructed and the pattern of chromosomal bands is restored in less than 48 hours. Cell growth arrests after irradiation but, while DNA is completely fragmented, RNA maintains its integrity. In this work we compared the transcriptional profiles of irradiated and non-irradiated epimastigotes at different time points after irradiation using microarray. In total, 273 genes were differentially expressed; from these, 160 were up-regulated and 113 down-regulated. We found that genes with predicted functions are the most prevalent in the down-regulated gene category. Translation and protein metabolic processes, as well as generation of precursor of metabolites and energy pathways were affected. In contrast, the up-regulated category was mainly composed of obsolete sequences (which included some genes of the kinetoplast DNA), genes coding for hypothetical proteins, and Retrotransposon Hot Spot genes. Finally, the tyrosyl-DNA phosphodiesterase 1, a gene involved in double-strand DNA break repair process, was up-regulated. Our study demonstrated the peculiar response to ionizing radiation, raising questions about how this organism changes its gene expression to manage such a harmful stress.DNA repairGamma radiation Gene expressionGenomic databases Ionizing radiationMicroarrays RNA extractionTrypanosoma cruziinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALTrypanosoma cruzi gene expression in response to gamma radiation.pdfTrypanosoma cruzi gene expression in response to gamma radiation.pdfapplication/pdf1836923https://www.arca.fiocruz.br/bitstream/icict/7593/1/Trypanosoma%20cruzi%20gene%20expression%20in%20response%20to%20gamma%20radiation.pdfe3ffa4dc31ec7bfb1f0c0c0dd96d7573MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7593/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTTrypanosoma cruzi gene expression in response to gamma radiation.pdf.txtTrypanosoma cruzi gene expression in response to gamma radiation.pdf.txtExtracted texttext/plain68043https://www.arca.fiocruz.br/bitstream/icict/7593/5/Trypanosoma%20cruzi%20gene%20expression%20in%20response%20to%20gamma%20radiation.pdf.txt4e9e0e4bd693765d71db62ae00236623MD55THUMBNAILTrypanosoma cruzi gene expression in response to gamma radiation.pdf.jpgTrypanosoma cruzi gene expression in response to gamma radiation.pdf.jpgGenerated Thumbnailimage/jpeg2090https://www.arca.fiocruz.br/bitstream/icict/7593/4/Trypanosoma%20cruzi%20gene%20expression%20in%20response%20to%20gamma%20radiation.pdf.jpg20acc1bb85caca264bf6a590dbee4b8eMD54icict/75932018-04-06 08:43:53.861oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:43:53Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Trypanosoma cruzi gene expression in response to gamma radiation
title Trypanosoma cruzi gene expression in response to gamma radiation
spellingShingle Trypanosoma cruzi gene expression in response to gamma radiation
Grynberg, Priscila
DNA repair
Gamma radiation Gene expression
Genomic databases Ionizing radiation
Microarrays RNA extraction
Trypanosoma cruzi
title_short Trypanosoma cruzi gene expression in response to gamma radiation
title_full Trypanosoma cruzi gene expression in response to gamma radiation
title_fullStr Trypanosoma cruzi gene expression in response to gamma radiation
title_full_unstemmed Trypanosoma cruzi gene expression in response to gamma radiation
title_sort Trypanosoma cruzi gene expression in response to gamma radiation
author Grynberg, Priscila
author_facet Grynberg, Priscila
Silva, Danielle Gomes Passos
Mourão, Marina de Moraes
Hirata Junior, Roberto
Macedo, Andrea Mara
Machado, Carlos Renato
Bartholomeu, Daniella Castanheira
Franco, Glória Regina
author_role author
author2 Silva, Danielle Gomes Passos
Mourão, Marina de Moraes
Hirata Junior, Roberto
Macedo, Andrea Mara
Machado, Carlos Renato
Bartholomeu, Daniella Castanheira
Franco, Glória Regina
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Grynberg, Priscila
Silva, Danielle Gomes Passos
Mourão, Marina de Moraes
Hirata Junior, Roberto
Macedo, Andrea Mara
Machado, Carlos Renato
Bartholomeu, Daniella Castanheira
Franco, Glória Regina
dc.subject.en.pt_BR.fl_str_mv DNA repair
Gamma radiation Gene expression
Genomic databases Ionizing radiation
Microarrays RNA extraction
Trypanosoma cruzi
topic DNA repair
Gamma radiation Gene expression
Genomic databases Ionizing radiation
Microarrays RNA extraction
Trypanosoma cruzi
description Universidade Federal de Minas Gerais. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brazil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-05-08T18:39:52Z
dc.date.available.fl_str_mv 2014-05-08T18:39:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv GRYNBERG, Priscila et al. Trypanosoma cruzi gene expression in response to gamma radiation. Plos One. 2012, vol.7, pp. e29596.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7593
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0029596
identifier_str_mv GRYNBERG, Priscila et al. Trypanosoma cruzi gene expression in response to gamma radiation. Plos One. 2012, vol.7, pp. e29596.
10.1371/journal.pone.0029596
url https://www.arca.fiocruz.br/handle/icict/7593
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Public Library Of Science
publisher.none.fl_str_mv Public Library Of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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