Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27094 |
Resumo: | Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. |
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Nogueira, Rita de Maria SeabraSilva, Arannadia BarbosaSato, Tayra PereiraSá, Joicy Cortez deSantos, Ana Clara Gomes dosAmorim Filho, Edvaldo FrancoVale, Tássia Lopes doGazeta, Gilberto Salles2018-06-26T11:53:12Z2018-06-26T11:53:12Z2017NOGUEIRA, Rita de Maria Seabra; et al. Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. Pesquisa Veterinária Brasileira, v.37, n.12, p.1416-1422, 2017.0100-736Xhttps://www.arca.fiocruz.br/handle/icict/2709410.1590/S0100-736X20170012000101678-5150engColégio Brasileiro de Patologia AnimalCavalosAnaplasma phagocytophilumCarrapatosBrasilBabesia caballiTheileria equiDoença transmitida por vetorVector-borne diseaseTheileria equiBabesia caballiAnaplasma phagocytophilumhorsesticksBrazilMolecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Universidade Federal de Juiz de Fora. Programa de Pós-Graduação em Comportamento e Biologia Animal. Juiz de Fora, MG, Brasil.Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil.Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil.Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil.Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Referência Nacional em Vetores das Riquetsioses. Rio de Janeiro, RJ. Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Programa de Pós-Graduação em Biodiversidade e Saúde. Rio de Janeiro, RJ, BrasilEquine piroplasmosis is a tick-borne disease caused by the intraeytrhocytic protozoans Babesia caballi and Theileria equi. It has been reported as a main equine parasitic disease. In addition, Anaplasma phagocytophilum, the causative agent of granulocytic ehrlichiosis, causes a seasonal disease in horses. Both diseases, can be detrimental to animal health. In this sense, blood samples and ticks were collected from 97 horses raised in the microregion of Baixada Maranhense, Maranhão State, Brazil. Serum samples were subjected to Indirect Fluorescence Antibody Test (IFAT) and blood samples and ticks to Polymerase Chain Reaction (PCR) to evaluate the infection by Theileria equi, Babesia caballi and Anaplasma phagocytophilum. The overall seroprevalence was 38.14%, 18.55% and 11.34% for T. equi, B. caballi and A. phagocytophilum, respectively. The results of PCR from blood samples showed 13.40% and 3.09% positive samples to T. equi and B. caballi, respectively. A total of 170 tick specimens were collected and identified as Dermacentor nitens, Amblyomma cajennense sensu lato and Rhipicephalus (Boophilus) microplus. It was detected 2.35% (4/170) and 0.59% (1/170) positive tick samples by PCR for T. equi and B. caballi, respectively. All samples were negative to A. phagocytophilum. No statically difference (p>0.05) was observed when gender, age, use of ectoparasiticide and tick presence were analyzed. A BLASTn analysis of the sequenced samples indicated 97 to 100% similarity with T. equi 18S rRNA gene sequences in GenBank and 98 to 100% with B. caballi. Genetic analysis classified the obtained sequences as T. equi and B. caballi cluster, respectively. It can be concluded that these pathogens occur and are circulating in the studied area.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27094/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALritamaria_nogueira_etal_IOC_2017.pdfritamaria_nogueira_etal_IOC_2017.pdfapplication/pdf1058551https://www.arca.fiocruz.br/bitstream/icict/27094/2/ritamaria_nogueira_etal_IOC_2017.pdfa41daf0661a4b0e7f42d597850a716caMD52TEXTritamaria_nogueira_etal_IOC_2017.pdf.txtritamaria_nogueira_etal_IOC_2017.pdf.txtExtracted texttext/plain35920https://www.arca.fiocruz.br/bitstream/icict/27094/3/ritamaria_nogueira_etal_IOC_2017.pdf.txt018077eca6ea92a1a0f27580c7adee53MD53icict/270942020-07-17 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| dc.title.pt_BR.fl_str_mv |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| title |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| spellingShingle |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil Nogueira, Rita de Maria Seabra Cavalos Anaplasma phagocytophilum Carrapatos Brasil Babesia caballi Theileria equi Doença transmitida por vetor Vector-borne disease Theileria equi Babesia caballi Anaplasma phagocytophilum horses ticks Brazil |
| title_short |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| title_full |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| title_fullStr |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| title_full_unstemmed |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| title_sort |
Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil |
| author |
Nogueira, Rita de Maria Seabra |
| author_facet |
Nogueira, Rita de Maria Seabra Silva, Arannadia Barbosa Sato, Tayra Pereira Sá, Joicy Cortez de Santos, Ana Clara Gomes dos Amorim Filho, Edvaldo Franco Vale, Tássia Lopes do Gazeta, Gilberto Salles |
| author_role |
author |
| author2 |
Silva, Arannadia Barbosa Sato, Tayra Pereira Sá, Joicy Cortez de Santos, Ana Clara Gomes dos Amorim Filho, Edvaldo Franco Vale, Tássia Lopes do Gazeta, Gilberto Salles |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nogueira, Rita de Maria Seabra Silva, Arannadia Barbosa Sato, Tayra Pereira Sá, Joicy Cortez de Santos, Ana Clara Gomes dos Amorim Filho, Edvaldo Franco Vale, Tássia Lopes do Gazeta, Gilberto Salles |
| dc.subject.other.pt_BR.fl_str_mv |
Cavalos Anaplasma phagocytophilum Carrapatos Brasil Babesia caballi Theileria equi Doença transmitida por vetor |
| topic |
Cavalos Anaplasma phagocytophilum Carrapatos Brasil Babesia caballi Theileria equi Doença transmitida por vetor Vector-borne disease Theileria equi Babesia caballi Anaplasma phagocytophilum horses ticks Brazil |
| dc.subject.en.pt_BR.fl_str_mv |
Vector-borne disease Theileria equi Babesia caballi Anaplasma phagocytophilum horses ticks Brazil |
| description |
Universidade Estadual do Maranhão. Departamento de Patologia. São Luis, MA, Brasil. |
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2017 |
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2017 |
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2018-06-26T11:53:12Z |
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2018-06-26T11:53:12Z |
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NOGUEIRA, Rita de Maria Seabra; et al. Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. Pesquisa Veterinária Brasileira, v.37, n.12, p.1416-1422, 2017. |
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https://www.arca.fiocruz.br/handle/icict/27094 |
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0100-736X |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0100-736X2017001200010 |
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1678-5150 |
| identifier_str_mv |
NOGUEIRA, Rita de Maria Seabra; et al. Molecular and serological detection of Theileria equi, Babesia caballi and Anaplasma phagocytophilum in horses and ticks in Maranhão, Brazil. Pesquisa Veterinária Brasileira, v.37, n.12, p.1416-1422, 2017. 0100-736X 10.1590/S0100-736X2017001200010 1678-5150 |
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Colégio Brasileiro de Patologia Animal |
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Colégio Brasileiro de Patologia Animal |
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