Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil

Detalhes bibliográficos
Autor(a) principal: Almeida-Silva, Fernando
Data de Publicação: 2022
Outros Autores: Rabello, Vanessa Brito de Souza, Scramignon-Costa, Bruno de Souza, Zancopé-Oliveira, Rosely Maria, Macedo, Priscila Marques de, Almeida-Paes, Rodrigo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55662
Resumo: Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
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spelling Almeida-Silva, FernandoRabello, Vanessa Brito de SouzaScramignon-Costa, Bruno de SouzaZancopé-Oliveira, Rosely MariaMacedo, Priscila Marques deAlmeida-Paes, Rodrigo2022-11-16T19:12:57Z2022-11-16T19:12:57Z2022ALMEIDA-SILVA, Fernando et al. Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil. Journal of fungi, v. 8, n. 6, p. 1-11, 2022.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5566210.3390/jof8060604engMDPIBeyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em Dermatologia Infeciosa. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.In Brazil, sporotrichosis has transitioned from a rural to urban disease, driven by a shift in the initiation of infection from the accidental inoculation of organic matter to the traumatic implantation of the fungus by cats. Since the emergence of zoonotic sporotrichosis caused by Sporothrix brasiliensis, investigations have largely ignored the environmental habitat of the pathogen due to its association with domestic cats. Therefore, we investigated 18 environmental samples collected from rural areas of two cities where zoonotic sporotrichosis is endemic, but where domestic cats are scarce. We utilized traditional culture methods, and samples were also examined with two molecular methods used for the clinical diagnosis of sporotrichosis: a nested-PCR targeting the ITS region and a species-specific PCR targeting the calmodulin gene. No Sporothrix colonies were identified by traditional culture methods. However, the nested-PCR and the species-specific PCR for S. brasiliensis were positive for 18 and 5 samples, respectively. Sequencing revealed that positive results with the nested-PCR were due to non-specific amplification of other Ophiostomatales DNA, rather than Sporothrix spp. Three of the five amplicons from the species-specific PCR were suitable for sequencing and confirmed the presence of S. brasiliensis DNA. Hence, we confirmed that S. brasiliensis, as with other Sporothrix species, has an environmental habitat. Our findings underscore the challenges of nested-PCR for Sporothrix environmental studies and highlight that sequencing must follow PCR protocols to definitively identify Sporothrix spp. in environmental samples.PCRSporothrixMolecular biologySoilinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55662/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALBeyond_Fernando_Silva_etal_INI_2022.pdfBeyond_Fernando_Silva_etal_INI_2022.pdfapplication/pdf4628500https://www.arca.fiocruz.br/bitstream/icict/55662/2/Beyond_Fernando_Silva_etal_INI_2022.pdf63cf35b06a159e6dbee0db9ec565745aMD52icict/556622022-11-16 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dc.title.en_US.fl_str_mv Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
title Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
spellingShingle Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
Almeida-Silva, Fernando
PCR
Sporothrix
Molecular biology
Soil
title_short Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
title_full Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
title_fullStr Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
title_full_unstemmed Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
title_sort Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil
author Almeida-Silva, Fernando
author_facet Almeida-Silva, Fernando
Rabello, Vanessa Brito de Souza
Scramignon-Costa, Bruno de Souza
Zancopé-Oliveira, Rosely Maria
Macedo, Priscila Marques de
Almeida-Paes, Rodrigo
author_role author
author2 Rabello, Vanessa Brito de Souza
Scramignon-Costa, Bruno de Souza
Zancopé-Oliveira, Rosely Maria
Macedo, Priscila Marques de
Almeida-Paes, Rodrigo
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida-Silva, Fernando
Rabello, Vanessa Brito de Souza
Scramignon-Costa, Bruno de Souza
Zancopé-Oliveira, Rosely Maria
Macedo, Priscila Marques de
Almeida-Paes, Rodrigo
dc.subject.en.en_US.fl_str_mv PCR
Sporothrix
Molecular biology
Soil
topic PCR
Sporothrix
Molecular biology
Soil
description Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Micologia. Rio de Janeiro, RJ, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-11-16T19:12:57Z
dc.date.available.fl_str_mv 2022-11-16T19:12:57Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ALMEIDA-SILVA, Fernando et al. Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil. Journal of fungi, v. 8, n. 6, p. 1-11, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55662
dc.identifier.issn.en_US.fl_str_mv 2309-608X
dc.identifier.doi.none.fl_str_mv 10.3390/jof8060604
identifier_str_mv ALMEIDA-SILVA, Fernando et al. Beyond Domestic Cats: Environmental Detection of Sporothrix brasiliensis DNA in a Hyperendemic Area of Sporotrichosis in Rio de Janeiro State, Brazil. Journal of fungi, v. 8, n. 6, p. 1-11, 2022.
2309-608X
10.3390/jof8060604
url https://www.arca.fiocruz.br/handle/icict/55662
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55662/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/55662/2/Beyond_Fernando_Silva_etal_INI_2022.pdf
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