Quick electrochemical immunoassay for hantavirus detection based on biochar platform
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41652 |
Resumo: | Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. |
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Martins, GustavoGogola, Jeferson L.Caetano, Fabio R.Kalinke, CristianeJorge, Taíssa R.Santos, Claudia Nunes Duarte dosBergamini, Márcio F.Marcolino-Junior, Luiz H.2020-06-15T17:51:03Z2020-06-15T17:51:03Z2019MARTINS, Gustavo et al. Quick electrochemical immunoassay for hantavirus detection based on biochar platform. Talanta, n. 204, p. 163-171, 2019.0039-9140https://www.arca.fiocruz.br/handle/icict/4165210.1016/j.talanta.2019.05.101porElsevierHCPSSíndrome Cardiopulmonar do HantavírusImunossensor EletroquímicoTeste RápidoBiocharHantavirus Cardiopulmonary SyndromeElectrochemical ImmunosensorQuick TestBiomassBiomasaHantavirusBiomassaQuick electrochemical immunoassay for hantavirus detection based on biochar platforminfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil.This work describes the first method using biochar (BC) as carbonaceous platform for immunoassay application. BC is a highly functionalized material obtained through biomass pyrolysis under controlled conditions. Due to the highly functionalized surface, covalent binding between BC and biomolecules can be performed by EDC/NHS conjugation. The application of the modified electrode was done with Hantavirus, that are etiologic agents mainly transmitted by wild rodents. Among its pathologies Hantavirus Cardiopulmonary Syndrome (HCPS) arises at Americas, caused by Hantavirus Araucária and reaches 40% lethality. The diagnostic is based on the presence of specific hantavirus nucleoprotein (Np), under viremic condition or IgG2b antibodies (Ab), during first symptoms. The results presented a device sensitivity of 5.28 μA dec-1 and a LOD of 0.14 ng mL-1 to the Np detection, ranging from 5.0 ng mL-1 to 1.0 μg mL-1, the Ab detection works as qualitative type sensor above 200 ng mL-1. Both sensors were evaluated its selectivity and serum samples; selectivity against Gumboro disease, VP2 protein, and antibody IgG2a against Yellow fever disease (YF), respectively. So, the devices here proposed are promising tool suitable for both rodent and human hantavirus clinical surveys.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/41652/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALICC - martins201.pdfICC - martins201.pdfapplication/pdf1199405https://www.arca.fiocruz.br/bitstream/icict/41652/2/ICC%20-%20martins201.pdfaa6e38162166575f0d4bd03f0fd53915MD52TEXTICC - martins201.pdf.txtICC - martins201.pdf.txtExtracted texttext/plain43227https://www.arca.fiocruz.br/bitstream/icict/41652/3/ICC%20-%20martins201.pdf.txtc85dba867ba04fb9e824d5805b6964a7MD53icict/416522022-04-28 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| dc.title.pt_BR.fl_str_mv |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| title |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| spellingShingle |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform Martins, Gustavo HCPS Síndrome Cardiopulmonar do Hantavírus Imunossensor Eletroquímico Teste Rápido Biochar Hantavirus Cardiopulmonary Syndrome Electrochemical Immunosensor Quick Test Biomass Biomasa Hantavirus Biomassa |
| title_short |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| title_full |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| title_fullStr |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| title_full_unstemmed |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| title_sort |
Quick electrochemical immunoassay for hantavirus detection based on biochar platform |
| author |
Martins, Gustavo |
| author_facet |
Martins, Gustavo Gogola, Jeferson L. Caetano, Fabio R. Kalinke, Cristiane Jorge, Taíssa R. Santos, Claudia Nunes Duarte dos Bergamini, Márcio F. Marcolino-Junior, Luiz H. |
| author_role |
author |
| author2 |
Gogola, Jeferson L. Caetano, Fabio R. Kalinke, Cristiane Jorge, Taíssa R. Santos, Claudia Nunes Duarte dos Bergamini, Márcio F. Marcolino-Junior, Luiz H. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Martins, Gustavo Gogola, Jeferson L. Caetano, Fabio R. Kalinke, Cristiane Jorge, Taíssa R. Santos, Claudia Nunes Duarte dos Bergamini, Márcio F. Marcolino-Junior, Luiz H. |
| dc.subject.other.pt_BR.fl_str_mv |
HCPS Síndrome Cardiopulmonar do Hantavírus Imunossensor Eletroquímico Teste Rápido Biochar |
| topic |
HCPS Síndrome Cardiopulmonar do Hantavírus Imunossensor Eletroquímico Teste Rápido Biochar Hantavirus Cardiopulmonary Syndrome Electrochemical Immunosensor Quick Test Biomass Biomasa Hantavirus Biomassa |
| dc.subject.en.pt_BR.fl_str_mv |
Hantavirus Cardiopulmonary Syndrome Electrochemical Immunosensor Quick Test Biomass |
| dc.subject.es.pt_BR.fl_str_mv |
Biomasa |
| dc.subject.decs.pt_BR.fl_str_mv |
Hantavirus Biomassa |
| description |
Universidade Federal do Paraná. Departamento de Química. Laboratório de Sensores Eletroquímicos. Curitiba, PR, Brasil. |
| publishDate |
2019 |
| dc.date.issued.fl_str_mv |
2019 |
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2020-06-15T17:51:03Z |
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2020-06-15T17:51:03Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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| dc.identifier.citation.fl_str_mv |
MARTINS, Gustavo et al. Quick electrochemical immunoassay for hantavirus detection based on biochar platform. Talanta, n. 204, p. 163-171, 2019. |
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https://www.arca.fiocruz.br/handle/icict/41652 |
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0039-9140 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.talanta.2019.05.101 |
| identifier_str_mv |
MARTINS, Gustavo et al. Quick electrochemical immunoassay for hantavirus detection based on biochar platform. Talanta, n. 204, p. 163-171, 2019. 0039-9140 10.1016/j.talanta.2019.05.101 |
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Elsevier |
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Elsevier |
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