Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Detalhes bibliográficos
Autor(a) principal: Carvalho, Gardenia Braz Figueiredo
Data de Publicação: 2011
Outros Autores: Pereira, Rosiane Aparecida da Silva, Pacífico, Lucila Grossi Gonçalves, Fonseca, Cristina Toscano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8271
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.
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spelling Carvalho, Gardenia Braz FigueiredoPereira, Rosiane Aparecida da SilvaPacífico, Lucila Grossi GonçalvesFonseca, Cristina Toscano2014-08-26T17:45:22Z2014-08-26T17:45:22Z2011CARVALHO, Gardenia Braz Figueiredo et al. Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis. Memorias do Instituto Oswaldo Cruz, 106(7): 837-843, 20110074-0276https://www.arca.fiocruz.br/handle/icict/827110.1590/S0074-02762011000700009engFundação Oswaldo CruzIdentification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Instituto Nacional de Ciências e Tecnologia em Doenças Tropicais Belo Horizonte, MG, BrasilThe development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.Schistosoma mansonidiagnosisantigen candidatebioinformatics analysisinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8271/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINAL. Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis.pdf. 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dc.title.pt_BR.fl_str_mv Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
spellingShingle Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
Carvalho, Gardenia Braz Figueiredo
Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
title_short Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_full Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_fullStr Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_full_unstemmed Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_sort Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
author Carvalho, Gardenia Braz Figueiredo
author_facet Carvalho, Gardenia Braz Figueiredo
Pereira, Rosiane Aparecida da Silva
Pacífico, Lucila Grossi Gonçalves
Fonseca, Cristina Toscano
author_role author
author2 Pereira, Rosiane Aparecida da Silva
Pacífico, Lucila Grossi Gonçalves
Fonseca, Cristina Toscano
author2_role author
author
author
dc.contributor.author.fl_str_mv Carvalho, Gardenia Braz Figueiredo
Pereira, Rosiane Aparecida da Silva
Pacífico, Lucila Grossi Gonçalves
Fonseca, Cristina Toscano
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
topic Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-08-26T17:45:22Z
dc.date.available.fl_str_mv 2014-08-26T17:45:22Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CARVALHO, Gardenia Braz Figueiredo et al. Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis. Memorias do Instituto Oswaldo Cruz, 106(7): 837-843, 2011
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8271
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762011000700009
identifier_str_mv CARVALHO, Gardenia Braz Figueiredo et al. Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis. Memorias do Instituto Oswaldo Cruz, 106(7): 837-843, 2011
0074-0276
10.1590/S0074-02762011000700009
url https://www.arca.fiocruz.br/handle/icict/8271
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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