Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis

Detalhes bibliográficos
Autor(a) principal: Carvalho,Gardenia Braz Figueiredo
Data de Publicação: 2011
Outros Autores: Silva-Pereira,Rosiane Aparecida da, Pacífico,Lucila Grossi Gonçalves, Fonseca,Cristina Toscano
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700009
Resumo: The development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.
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spelling Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasisSchistosoma mansonidiagnosisantigen candidatebioinformatics analysisThe development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.Instituto Oswaldo Cruz, Ministério da Saúde2011-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700009Memórias do Instituto Oswaldo Cruz v.106 n.7 2011reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762011000700009info:eu-repo/semantics/openAccessCarvalho,Gardenia Braz FigueiredoSilva-Pereira,Rosiane Aparecida daPacífico,Lucila Grossi GonçalvesFonseca,Cristina Toscanoeng2020-04-25T17:51:04Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:17:53.984Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
spellingShingle Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
Carvalho,Gardenia Braz Figueiredo
Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
title_short Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_full Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_fullStr Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_full_unstemmed Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
title_sort Identification of Schistosoma mansoni candidate antigens for diagnosis of schistosomiasis
author Carvalho,Gardenia Braz Figueiredo
author_facet Carvalho,Gardenia Braz Figueiredo
Silva-Pereira,Rosiane Aparecida da
Pacífico,Lucila Grossi Gonçalves
Fonseca,Cristina Toscano
author_role author
author2 Silva-Pereira,Rosiane Aparecida da
Pacífico,Lucila Grossi Gonçalves
Fonseca,Cristina Toscano
author2_role author
author
author
dc.contributor.author.fl_str_mv Carvalho,Gardenia Braz Figueiredo
Silva-Pereira,Rosiane Aparecida da
Pacífico,Lucila Grossi Gonçalves
Fonseca,Cristina Toscano
dc.subject.por.fl_str_mv Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
topic Schistosoma mansoni
diagnosis
antigen candidate
bioinformatics analysis
dc.description.none.fl_txt_mv The development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.
description The development of a more sensitive diagnostic test for schistosomiasis is needed to overcome the limitations of the use of stool examination in low endemic areas. Using parasite antigens in enzyme linked immunosorbent assay is a promising strategy, however a more rational selection of parasite antigens is necessary. In this study we performed in silico analysis of the Schistosoma mansoni genome, using SchistoDB database and bioinformatic tools for screening immunogenic antigens. Based on evidence of expression in all parasite life stage within the definitive host, extracellular or plasmatic membrane localization, low similarity to human and other helminthic proteins and presence of predicted B cell epitopes, six candidates were selected: a glycosylphosphatidylinositol-anchored 200 kDa protein, two putative cytochrome oxidase subunits, two expressed proteins and one hypothetical protein. The recognition in unidimensional and bidimensional Western blot of protein with similar molecular weight and isoelectric point to the selected antigens by sera from S. mansoni infected mice indicate a good correlation between these two approaches in selecting immunogenic proteins.
publishDate 2011
dc.date.none.fl_str_mv 2011-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700009
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762011000700009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762011000700009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.106 n.7 2011
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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