Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin

Detalhes bibliográficos
Autor(a) principal: Bailey, James B.
Data de Publicação: 1986
Outros Autores: Michelson, Edward H., Paraense, W. Lobato
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42795
Resumo: É descrito um método simples e rápido para distinguir as espécies crípticas Biomphalaria tenagophila e B. occidentalis por eletroforese em gel de agarose. A prova é feita com hemolinfa do molusco, permitindo a cor vermelha da fração hemoglobina visualizar os padrões de migração sem necessidade de recorrer a colorações específicas. Além disso, as amostras de hemolinfa podem ser obtidas sem sacrificar o molusco, que poderá ser usado para outros estudos ou para criação.Bio
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spelling Bailey, James B.Michelson, Edward H.Paraense, W. Lobato2020-08-17T14:50:18Z2020-08-17T14:50:18Z1986BAILEY, James B. et al. Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 81, n. 3, p. 319-322, jul./set. 1986.0074-0276https://www.arca.fiocruz.br/handle/icict/4279510.1590/S0074-027619860003000081678-8060É descrito um método simples e rápido para distinguir as espécies crípticas Biomphalaria tenagophila e B. occidentalis por eletroforese em gel de agarose. A prova é feita com hemolinfa do molusco, permitindo a cor vermelha da fração hemoglobina visualizar os padrões de migração sem necessidade de recorrer a colorações específicas. Além disso, as amostras de hemolinfa podem ser obtidas sem sacrificar o molusco, que poderá ser usado para outros estudos ou para criação.BioA simple and rapid method for differentialing the sibling species Biomphalaria tenagophila and Biomphalaria occidentalis by agarose gel electrophoresis (AGE) is described. Snail hemolymph is used as the test sample and the red colaration of the hemoglobin fraction permits visualization of the migration patterns without resorting to specific stains. Moreover, hemolymph samples may be obtained without killing the snail, thus permitting its use for other studies for breeding.University of the Health Sciences. Betheda, USA.University of the Health Sciences. Uniformed Services. Department of Preventive Medicine and Biometrics. Betheda, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Malacologia. Rio de Janeiro, RJ, Brasil.engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Biomphalaria OccidentalisBiomphalaria TenagophilaEspécie irmãHemoglobinaEletroforeseTaxonomia bioquímicaBiomphalaria OccidentalisBiomphalaria TenagophilaSibiling speciesHemoglobinElectrophoresisBiochemical taxonomyDifferentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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dc.title.pt_BR.fl_str_mv Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
title Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
spellingShingle Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
Bailey, James B.
Biomphalaria Occidentalis
Biomphalaria Tenagophila
Espécie irmã
Hemoglobina
Eletroforese
Taxonomia bioquímica
Biomphalaria Occidentalis
Biomphalaria Tenagophila
Sibiling species
Hemoglobin
Electrophoresis
Biochemical taxonomy
title_short Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
title_full Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
title_fullStr Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
title_full_unstemmed Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
title_sort Differentation of the sibiling species Biomphalaria Occidentalis and Biomphalaria Tenagophila by the electrophoretic patterns of their hemoglobin
author Bailey, James B.
author_facet Bailey, James B.
Michelson, Edward H.
Paraense, W. Lobato
author_role author
author2 Michelson, Edward H.
Paraense, W. Lobato
author2_role author
author
dc.contributor.author.fl_str_mv Bailey, James B.
Michelson, Edward H.
Paraense, W. Lobato
dc.subject.other.pt_BR.fl_str_mv Biomphalaria Occidentalis
Biomphalaria Tenagophila
Espécie irmã
Hemoglobina
Eletroforese
Taxonomia bioquímica
topic Biomphalaria Occidentalis
Biomphalaria Tenagophila
Espécie irmã
Hemoglobina
Eletroforese
Taxonomia bioquímica
Biomphalaria Occidentalis
Biomphalaria Tenagophila
Sibiling species
Hemoglobin
Electrophoresis
Biochemical taxonomy
dc.subject.en.pt_BR.fl_str_mv Biomphalaria Occidentalis
Biomphalaria Tenagophila
Sibiling species
Hemoglobin
Electrophoresis
Biochemical taxonomy
description É descrito um método simples e rápido para distinguir as espécies crípticas Biomphalaria tenagophila e B. occidentalis por eletroforese em gel de agarose. A prova é feita com hemolinfa do molusco, permitindo a cor vermelha da fração hemoglobina visualizar os padrões de migração sem necessidade de recorrer a colorações específicas. Além disso, as amostras de hemolinfa podem ser obtidas sem sacrificar o molusco, que poderá ser usado para outros estudos ou para criação.Bio
publishDate 1986
dc.date.issued.fl_str_mv 1986
dc.date.accessioned.fl_str_mv 2020-08-17T14:50:18Z
dc.date.available.fl_str_mv 2020-08-17T14:50:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BAILEY, James B. et al. Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 81, n. 3, p. 319-322, jul./set. 1986.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42795
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761986000300008
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BAILEY, James B. et al. Differentiation of the sibling species Biomphalaria occidentalis and Biomphalaria tenagophila by the electrophoretic patterns of their hemoglobin. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 81, n. 3, p. 319-322, jul./set. 1986.
0074-0276
10.1590/S0074-02761986000300008
1678-8060
url https://www.arca.fiocruz.br/handle/icict/42795
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
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