HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.

Detalhes bibliográficos
Autor(a) principal: Silberstein, Erica
Data de Publicação: 2016
Outros Autores: Ulitzky, Laura, Lima, Livia Alves, Cehan, Nicoleta, Carvalho, Andréa Teixeira de, Roingeard, Philippe, Taylor, Deborah R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/18430
Resumo: Office of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of America
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spelling Silberstein, EricaUlitzky, LauraLima, Livia AlvesCehan, NicoletaCarvalho, Andréa Teixeira deRoingeard, PhilippeTaylor, Deborah R.2017-04-12T18:12:20Z2017-04-12T18:12:20Z2016SILBERSTEIN, Erica et al. HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis. PLoS One, v. 11, n. 6, e0155708, 2016.1932-6203https://www.arca.fiocruz.br/handle/icict/1843010.1371/journal.pone.0155708engPublic Library of ScienceApoptosisApoptosisGene expressionCell stainingDNA-binding proteinsFlow cytometryProteasesHepatitis C virusNecrosisHCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleOffice of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaOffice of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaFederal University of Mato Grosso do Sul. Campo Grande, MT, Brazil/ Office of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaOffice of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Grupo Integrado de Pesquisas em Biomarcadores. Belo Horizonte, MG, Brazil/Office of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaUniversite Francois Rabelais and CHRU de Tours. Tours, FranceOffice of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of AmericaChronic Hepatitis C Virus (HCV) infection is associated with progressive liver injury and subsequent development of fibrosis and cirrhosis. The death of hepatocytes results in the release of cytokines that induce inflammatory and fibrotic responses. The mechanism of liver damage is still under investigation but both apoptosis and immune-mediated processes may play roles. By observing the changes in gene expression patterns in HCV-infected cells, both markers and the causes of HCV-associated liver injury may be elucidated. HCV genotype 1b virus from persistently infected VeroE6 cells induced a strong cytopathic effect when used to infect Huh7.5 hepatoma cells. To determine if this cytopathic effect was a result of apoptosis, ultrastructural changes were observed by electron microscopy and markers of programmed cell death were surveyed. Screening of a human PCR array demonstrated a gene expression profile that contained upregulated markers of apoptosis, including tumor necrosis factor, caspases and caspase activators, Fas, Bcl2-interacting killer (BIK) and tumor suppressor protein, p53, as a result of HCV genotype 1b infection. The genes identified in this study should provide new insights into understanding viral pathogenesis in liver cells and may possibly help to identify novel antiviral and antifibrotic targets.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/18430/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALve_Silberstein_Erica_HCV-Mediated_CPqRR_2016 .pdfve_Silberstein_Erica_HCV-Mediated_CPqRR_2016 .pdfapplication/pdf7052667https://www.arca.fiocruz.br/bitstream/icict/18430/2/ve_Silberstein_Erica_HCV-Mediated_CPqRR_2016%20.pdfd507b556a2b76a53d87edb28918442d8MD52TEXTve_Silberstein_Erica_HCV-Mediated_CPqRR_2016 .pdf.txtve_Silberstein_Erica_HCV-Mediated_CPqRR_2016 .pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
title HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
spellingShingle HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
Silberstein, Erica
Apoptosis
Apoptosis
Gene expression
Cell staining
DNA-binding proteins
Flow cytometry
Proteases
Hepatitis C virus
Necrosis
title_short HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
title_full HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
title_fullStr HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
title_full_unstemmed HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
title_sort HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis.
author Silberstein, Erica
author_facet Silberstein, Erica
Ulitzky, Laura
Lima, Livia Alves
Cehan, Nicoleta
Carvalho, Andréa Teixeira de
Roingeard, Philippe
Taylor, Deborah R.
author_role author
author2 Ulitzky, Laura
Lima, Livia Alves
Cehan, Nicoleta
Carvalho, Andréa Teixeira de
Roingeard, Philippe
Taylor, Deborah R.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silberstein, Erica
Ulitzky, Laura
Lima, Livia Alves
Cehan, Nicoleta
Carvalho, Andréa Teixeira de
Roingeard, Philippe
Taylor, Deborah R.
dc.subject.other.pt_BR.fl_str_mv Apoptosis
topic Apoptosis
Apoptosis
Gene expression
Cell staining
DNA-binding proteins
Flow cytometry
Proteases
Hepatitis C virus
Necrosis
dc.subject.en.pt_BR.fl_str_mv Apoptosis
Gene expression
Cell staining
DNA-binding proteins
Flow cytometry
Proteases
Hepatitis C virus
Necrosis
description Office of Blood Research and Review. Division of Emerging Transfusion Transmitted Diseases. Laboratory of Emerging Pathogens. Silver Spring, MD, United States of America
publishDate 2016
dc.date.issued.fl_str_mv 2016
dc.date.accessioned.fl_str_mv 2017-04-12T18:12:20Z
dc.date.available.fl_str_mv 2017-04-12T18:12:20Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SILBERSTEIN, Erica et al. HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis. PLoS One, v. 11, n. 6, e0155708, 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/18430
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0155708
identifier_str_mv SILBERSTEIN, Erica et al. HCV-Mediated Apoptosis of Hepatocytes in Culture and Viral Pathogenesis. PLoS One, v. 11, n. 6, e0155708, 2016.
1932-6203
10.1371/journal.pone.0155708
url https://www.arca.fiocruz.br/handle/icict/18430
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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