Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery

Detalhes bibliográficos
Autor(a) principal: Bandeira, Elga
Data de Publicação: 2016
Outros Autores: Pacheco, Miquéias Lopes, Chiaramoni, Nadia, Ferreira, Débora, Fernandez-Ruocco, Maria J., Prieto, Maria J., Maron-Gutierrez, Tatiana, Perrotta, Ramiro M., Faria-Neto, Hugo C. de Castro, Rocco, Patricia R. M., Alonso, Silvia Del Valle, Morales, Marcelo M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16409
Resumo: Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Instituto de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.
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spelling Bandeira, ElgaPacheco, Miquéias LopesChiaramoni, NadiaFerreira, DéboraFernandez-Ruocco, Maria J.Prieto, Maria J.Maron-Gutierrez, TatianaPerrotta, Ramiro M.Faria-Neto, Hugo C. de CastroRocco, Patricia R. M.Alonso, Silvia Del ValleMorales, Marcelo M.2016-12-01T16:55:36Z2016-12-01T16:55:36Z2016BANDEIRA, Elga; et al. Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery. v.7, Article 151, 9p, Apr. 2016.1664-042X)https://www.arca.fiocruz.br/handle/icict/1640910.3389/fphys.2016.00151engFrontiers MediaPulmãoEntrega de genesParâmetros mecânicosNanopartículasInflamaçãolunggene deliverymechanic parametersnanoparticlesinflammationAssociation with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Deliveryinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Instituto de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Instituto de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.National University of Quilmes. Department of Science and Technology. Laboratory of Biomembranes. Buenos Aires, Argentina.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, BrasilNational University of Quilmes. Department of Science and Technology. Laboratory of Biomembranes. Buenos Aires, Argentina.National University of Quilmes. Department of Science and Technology. Laboratory of Biomembranes. Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.National University of Quilmes. Department of Science and Technology. Laboratory of Biomembranes. Buenos Aires, Argentina.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ. Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Instituto de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.National University of Quilmes. Department of Science and Technology. Laboratory of Biomembranes. Buenos Aires, Argentina.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil.Development of improved drug and gene delivery systems directly into the lungs is highly desirable given the important burden of respiratory diseases. We aimed to evaluate the safety and efficacy of liposomes composed of photopolymerized lipids [1,2-bis-(tricosa-10,12-diynoyl)-sn-glycero-3-phosphocholine] associated with amino acids as vectors for gene delivery into the lungs of healthy animals. Lipopolymer vesicles, in particular, are more stable than other types of liposomes. In this study, lipopolymers were associated with l-arginine, l-tryptophan, or l-cysteine. We hypothesized that the addition of these amino acids would enhance the efficacy of gene delivery to the lungs by the lipopolymers. l-Arginine showed the highest association efficiency due to its positive charge and better surface interactions. None of the formulations caused inflammation or altered lung mechanics, suggesting that these lipopolymers can be safely administered as aerosols. All formulations were able to induce eGFP mRNA expression in lung tissue, but the addition of amino acids reduced delivery efficacy when compared with the simple lipopolymer particle. These results indicate that this system could be further explored for gene or drug delivery targeting lung diseases.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/16409/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALtatiana_gutierrez_etal_IOC_2016.pdftatiana_gutierrez_etal_IOC_2016.pdfapplication/pdf1138826https://www.arca.fiocruz.br/bitstream/icict/16409/2/tatiana_gutierrez_etal_IOC_2016.pdf4c651d3263d78128d45be218b05abab1MD52TEXTtatiana_gutierrez_etal_IOC_2016.pdf.txttatiana_gutierrez_etal_IOC_2016.pdf.txtExtracted texttext/plain47910https://www.arca.fiocruz.br/bitstream/icict/16409/3/tatiana_gutierrez_etal_IOC_2016.pdf.txt76b34974041995845313a232dd420f7aMD53icict/164092018-08-15 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dc.title.pt_BR.fl_str_mv Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
title Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
spellingShingle Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
Bandeira, Elga
Pulmão
Entrega de genes
Parâmetros mecânicos
Nanopartículas
Inflamação
lung
gene delivery
mechanic parameters
nanoparticles
inflammation
title_short Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
title_full Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
title_fullStr Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
title_full_unstemmed Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
title_sort Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery
author Bandeira, Elga
author_facet Bandeira, Elga
Pacheco, Miquéias Lopes
Chiaramoni, Nadia
Ferreira, Débora
Fernandez-Ruocco, Maria J.
Prieto, Maria J.
Maron-Gutierrez, Tatiana
Perrotta, Ramiro M.
Faria-Neto, Hugo C. de Castro
Rocco, Patricia R. M.
Alonso, Silvia Del Valle
Morales, Marcelo M.
author_role author
author2 Pacheco, Miquéias Lopes
Chiaramoni, Nadia
Ferreira, Débora
Fernandez-Ruocco, Maria J.
Prieto, Maria J.
Maron-Gutierrez, Tatiana
Perrotta, Ramiro M.
Faria-Neto, Hugo C. de Castro
Rocco, Patricia R. M.
Alonso, Silvia Del Valle
Morales, Marcelo M.
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bandeira, Elga
Pacheco, Miquéias Lopes
Chiaramoni, Nadia
Ferreira, Débora
Fernandez-Ruocco, Maria J.
Prieto, Maria J.
Maron-Gutierrez, Tatiana
Perrotta, Ramiro M.
Faria-Neto, Hugo C. de Castro
Rocco, Patricia R. M.
Alonso, Silvia Del Valle
Morales, Marcelo M.
dc.subject.other.pt_BR.fl_str_mv Pulmão
Entrega de genes
Parâmetros mecânicos
Nanopartículas
Inflamação
topic Pulmão
Entrega de genes
Parâmetros mecânicos
Nanopartículas
Inflamação
lung
gene delivery
mechanic parameters
nanoparticles
inflammation
dc.subject.en.pt_BR.fl_str_mv lung
gene delivery
mechanic parameters
nanoparticles
inflammation
description Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Fisiologia Celular e Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Instituto de Investigação Pulmonar. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-12-01T16:55:36Z
dc.date.available.fl_str_mv 2016-12-01T16:55:36Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BANDEIRA, Elga; et al. Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery. v.7, Article 151, 9p, Apr. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16409
dc.identifier.issn.pt_BR.fl_str_mv 1664-042X)
dc.identifier.doi.none.fl_str_mv 10.3389/fphys.2016.00151
identifier_str_mv BANDEIRA, Elga; et al. Association with Amino Acids Does Not Enhance Efficacy of Polymerized Liposomes As a System for Lung Gene Delivery. v.7, Article 151, 9p, Apr. 2016.
1664-042X)
10.3389/fphys.2016.00151
url https://www.arca.fiocruz.br/handle/icict/16409
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16409/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/16409/2/tatiana_gutierrez_etal_IOC_2016.pdf
https://www.arca.fiocruz.br/bitstream/icict/16409/3/tatiana_gutierrez_etal_IOC_2016.pdf.txt
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