Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).

Detalhes bibliográficos
Autor(a) principal: Marques, Sirlei Garcia
Data de Publicação: 2008
Outros Autores: Silva, Conceição de Maria Pedroso e, Resende, Maria Aparecida, Silva, Antonio Augusto Moura da, Caldas, Arlene de Jesus Mendes, Costa, Jackson Mauricio Lopes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/9803
Resumo: Federal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, Brasil
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spelling Marques, Sirlei GarciaSilva, Conceição de Maria Pedroso eResende, Maria AparecidaSilva, Antonio Augusto Moura daCaldas, Arlene de Jesus MendesCosta, Jackson Mauricio Lopes2015-03-23T13:16:47Z2015-03-23T13:16:47Z2008MARQUES, S. G. et al. Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin). Japanese Journal of Mycology, v. 49, n. 2, p.95-101, 2008.0916-4804https://www.arca.fiocruz.br/handle/icict/9803engJapanese Journal of Medical MycologyDetection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFederal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, BrasilFederal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, BrasilFederal University of Minas Gerais. Institute of Biological Sciences. Department of Microbiology. Belo Horizonte, MG, BrasilFederal University of Maranhão. Department of Public Health. São Luis, MA, BrasilFederal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilAn experimental study was conducted between January 2002 and April 2003 for the detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin) in skin tests. A total of 194 subjects were attended by spontaneous demand at the Infectious and Parasitic Diseases outpatient clinic of the Federal University of Maranhão-UFMA and at the Department of Microbiology, Federal University of Minas Gerais-UFMG and classified into three groups: patients with chromoblastomycosis caused by F. pedrosoi (n=20), healthy subjects (n=86) and patients with other diseases (n=88). For the skin test, 0.1 ml of the antigen was applied to the anterior side of the right forearm and 0.1 ml Smith medium was applied to the anterior side of the left forearm as control. The results were analyzed 48 h after inoculation of the antigen and an induration >/= 5 mm was considered to indicate a positive test. A cellular immune response to chromomycin was detected in 18 (90.0%) of the 20 patients with chromoblastomycosis caused by F. pedrosoi, and one of the patients with a negative test had reactional leprosy. Eighty-five (98.8%) of the 86 healthy subjects presented a negative reaction and only one reacted positively to the antigen. The skin test was negative in all 88 (100%) patients with other diseases, such as dermatophytosis, paracoccidioidomycosis, pulmonary aspergilloma, candidiasis, pityriasis versicolor, tuberculosis, leprosy, tegumentary leishmaniasis and syphilis, and one case of chromoblastomycosis caused by Rhinocladiella aquaspersa. Chromomycin was effective in detecting delayed hypersensitivity in patients with chromoblastomycosis caused by F. pedrosoi, with a sensitivity and specificity of 90.0% and 98.8%, respectively. These results suggest that this antigen can be used in the auxiliary diagnosis of the disease and also in epidemiological studies for determination of the prevalence of chromoblastomycosis infection in endemic areas.CromoblastomycosisFonsecaea pedrosoiChromomycinSkin testMetabolic antigenAntígenos de Fungos/imunologiaCromoblastomicose/diagnósticoCromomicinas/imunologiaHipersensibilidade TardiaPhialophora/imunologiaTestes Cutâneos/métodosAdultoIdosoAntígenos de Fungos/uso diagnósticoCromomicinas/uso diagnósticoFemininoHumanosMasculinoMeia-IdadeSensibilidade e Especificidadeinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82352https://www.arca.fiocruz.br/bitstream/icict/9803/1/license.txtafbdf7d7a9bcf771a1cc7edf5f618413MD51ORIGINALMarques SG Detection of delayed............pdfMarques SG Detection of delayed............pdfapplication/pdf77704https://www.arca.fiocruz.br/bitstream/icict/9803/2/Marques%20SG%20Detection%20of%20delayed............pdfc9b14a2e6f2c037cf0c24c410b106a78MD52TEXTMarques SG Detection of delayed............pdf.txtMarques SG Detection of delayed............pdf.txtExtracted texttext/plain27621https://www.arca.fiocruz.br/bitstream/icict/9803/3/Marques%20SG%20Detection%20of%20delayed............pdf.txt07cfe7b5db8c6fce2dbe47fdebaed284MD53icict/98032023-03-15 14:33:12.008oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:12Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
title Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
spellingShingle Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
Marques, Sirlei Garcia
Cromoblastomycosis
Fonsecaea pedrosoi
Chromomycin
Skin test
Metabolic antigen
Antígenos de Fungos/imunologia
Cromoblastomicose/diagnóstico
Cromomicinas/imunologia
Hipersensibilidade Tardia
Phialophora/imunologia
Testes Cutâneos/métodos
Adulto
Idoso
Antígenos de Fungos/uso diagnóstico
Cromomicinas/uso diagnóstico
Feminino
Humanos
Masculino
Meia-Idade
Sensibilidade e Especificidade
title_short Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
title_full Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
title_fullStr Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
title_full_unstemmed Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
title_sort Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin).
author Marques, Sirlei Garcia
author_facet Marques, Sirlei Garcia
Silva, Conceição de Maria Pedroso e
Resende, Maria Aparecida
Silva, Antonio Augusto Moura da
Caldas, Arlene de Jesus Mendes
Costa, Jackson Mauricio Lopes
author_role author
author2 Silva, Conceição de Maria Pedroso e
Resende, Maria Aparecida
Silva, Antonio Augusto Moura da
Caldas, Arlene de Jesus Mendes
Costa, Jackson Mauricio Lopes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marques, Sirlei Garcia
Silva, Conceição de Maria Pedroso e
Resende, Maria Aparecida
Silva, Antonio Augusto Moura da
Caldas, Arlene de Jesus Mendes
Costa, Jackson Mauricio Lopes
dc.subject.en.pt_BR.fl_str_mv Cromoblastomycosis
Fonsecaea pedrosoi
Chromomycin
Skin test
Metabolic antigen
topic Cromoblastomycosis
Fonsecaea pedrosoi
Chromomycin
Skin test
Metabolic antigen
Antígenos de Fungos/imunologia
Cromoblastomicose/diagnóstico
Cromomicinas/imunologia
Hipersensibilidade Tardia
Phialophora/imunologia
Testes Cutâneos/métodos
Adulto
Idoso
Antígenos de Fungos/uso diagnóstico
Cromomicinas/uso diagnóstico
Feminino
Humanos
Masculino
Meia-Idade
Sensibilidade e Especificidade
dc.subject.decs.pt_BR.fl_str_mv Antígenos de Fungos/imunologia
Cromoblastomicose/diagnóstico
Cromomicinas/imunologia
Hipersensibilidade Tardia
Phialophora/imunologia
Testes Cutâneos/métodos
Adulto
Idoso
Antígenos de Fungos/uso diagnóstico
Cromomicinas/uso diagnóstico
Feminino
Humanos
Masculino
Meia-Idade
Sensibilidade e Especificidade
description Federal University of Maranhão. Nucleus of Tropical Pathology and Social Medicine. Department of Pathology. São Luis, MA, Brasil
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2015-03-23T13:16:47Z
dc.date.available.fl_str_mv 2015-03-23T13:16:47Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MARQUES, S. G. et al. Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin). Japanese Journal of Mycology, v. 49, n. 2, p.95-101, 2008.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/9803
dc.identifier.issn.none.fl_str_mv 0916-4804
identifier_str_mv MARQUES, S. G. et al. Detection of delayed hypersensitivity to Fonsecaea pedrosoi metabolic antigen (chromomycin). Japanese Journal of Mycology, v. 49, n. 2, p.95-101, 2008.
0916-4804
url https://www.arca.fiocruz.br/handle/icict/9803
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Japanese Journal of Medical Mycology
publisher.none.fl_str_mv Japanese Journal of Medical Mycology
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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